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1.

001-es BibID:BIBFORM131775
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Propagation of [D1,2]-type spliceosomal twin introns (stwintrons) in Hypoxylaceae and Xylariaceae fungi / Erzsébet Fekete, Norbert Ág, Viktória Ág-Rácz, Alexandra Márton, Erzsébet Sándor, Claudio Scazzocchio, Michel Flipphi, Levente Karaffa
Dátum:2025
ISSN:2165-0497
Megjegyzések:[D1,2] stwintrons consist of nested U2 introns, where an internal intron splits the 5·-donor of an external intron between its first and second nucleotide. Almost all stwintrons described to date are of the [D1,2] type, suggesting unique means for their duplication. Sequence-similar [D1,2] stwintrons are typically integrated at new intron positions, specific for one species. Two hundred eighty-eight sequence-similar [D1,2] stwintrons were identified in the genomes of 14 Xylariales. Occasional missplicing was apparent, where almost the entire stwintron was excised as one canonical intron. Near-identical sister stwintrons were identified in Xylaria sp. MSU SB201401 and Xylaria longipes that share near-terminal inverted repeat elements of 10-nt length named 5·-NTIRE-10 and 3·-NTIRE-10, which are complementary as RNA. Complementary NTIRE-10 partners were also present in three Hypoxylaceae species. These NTIRE-10s can form a near-terminal double-stranded RNA stem structure that brings in close proximity the terminal G's of the [D1,2] stwintron and of its alternative misspliced intron. The exact folding of the interior stwintron RNA appears irrelevant. Ten of the stwintrons with complementary NTIRE-10s are present in Xylaria sp. MSU SB201401, implying that [D1,2] stwintron duplication occurs frequently in this species.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
spliceosomal introns
splicing
[D1,2] stwintron propagation
Xylariales
near-identical stwintrons
near-terminal double-stranded RNA stem structure
D1,2] missplicing using distal splice sites
Megjelenés:Microbiology Spectrum. - 13 : 9 (2025), p. 1-15. -
További szerzők:Ág Norbert (1987-) (okleveles biomérnök) Ág-Rácz Viktória (1991-) (mikrobiológus) Márton Alexandra (1994-) (mikrobiológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Scazzocchio, Claudio Flipphi, Michel (1964-) Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
Pályázati támogatás:K 146406
NKFIH
K 138489
NKFIH
Internet cím:DOI
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2.

001-es BibID:BIBFORM125016
035-os BibID:(Scopus)85208772584 (WoS)001330977100001
Első szerző:Fekete Erzsébet (biotechnológus, egyetemi tanár)
Cím:Transcriptomics identify the triggering of citrate export as the key event caused by manganese deficiency in Aspergillus niger / Erzsébet Fekete, Vivien Bíró, Alexandra Márton, István Bakondi-Kovács, Erzsébet Sándor, Béla Kovács, Nicholas Geoffrion, Adrian Tsang, Christian P. Kubicek, Levente Karaffa1
Dátum:2024
ISSN:2165-0497
Megjegyzések:For over a century, the filamentous Ascomycete fungus Aspergillus niger has played a pivotal role in the industrial production of citric acid. A critical fermentation parameter that sustains high-yield citric acid accumulation is the suboptimal concentration of manganese(II) ions in the culture broth at the early stages of the process. However, the requirement for this deficiency has not been investigated on a functional genomics level. In this study, we compared the transcriptome of the citric acid hyper-producer A. niger NRRL2270 strain grown under citric acid-producing conditions in 6-L scale bioreactors at Mn2+ ion-deficient (5 ppb) and Mn2+ ion-sufficient (100 ppb) conditions at three early time points of cultivation. Of the 11,846 genes in the genome, 963 genes (8.1% of the total) were identified as significantly differentially expressed under these conditions. Disproportionately high number of differentially regulated genes encode predicted extracellular and membrane proteins. The most abundant gene group that was upregulated in Mn2+ ion deficiency condition encodes enzymes acting on polysaccharides. In contrast, six clusters of genes encoding secondary metabolites showed downregulation under manganese deficiency. Mn2+ deficiency also triggers upregulation of the cexA gene, which encodes the citrate exporter. We provide functional evidence that the upregulation of cexA is caused by the intracellular accumulation of citrate or acetyl-CoA and is a major factor in triggering citrate overflow. IMPORTANCE Citric acid is produced on industrial scale by batch fermentation of the filamentous fungus Aspergillus niger. High-yield citric acid production requires a low (<5 ppb) manganese(II) ion concentration in the culture broth. However, the requirement for this deficiency has not been investigated on a functional genomics level. Here, we compared the transcriptome of a citric acid hyper-producer A. niger strain grown under citric acid-producing conditions in 6-L scale bioreactors at Mn2+ ion-deficient (5 ppb) and Mn2+ ion-sufficient (100 ppb) conditions at three early time points of cultivation. We observed that Mn2+ deficiency triggers an upregulation of the citrate exporter gene cexA and provides functional evidence that this event is responsible for citrate overflow. In addition to the industrial relevance, this is the first study that examined the role of Mn2+ ion deficiency in a heterotrophic eukaryotic cell on a genome-wide scale.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus niger
citric acid
manganese ions
citrate exporter
cexA
transcriptomics
gene expression
Megjelenés:Microbiology Spectrum. - 12 : 11 (2024), p. 1-18. -
További szerzők:Bíró Vivien (1994-)c(mikrobiológus) Márton Alexandra (1994-) (mikrobiológus) Bakondi-Kovács István (1963-) (mikrobiológus) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Kovács Béla (1963-) (mezőgazdasági kémia, angol szakfordító) Geoffrion, Nicholas Tsang, Adrian Kubicek, Christian P. Karaffa Levente (1971-) (biotechnológus, egyetemi tanár)
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DOI
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3.

001-es BibID:BIBFORM134280
035-os BibID:(WoS)001625454400001
Első szerző:Harmath Andrea (gyógyszer-biotechnológus)
Cím:Phylogenomic diversity of clinical Saccharomyces cerevisiae and the prevalence of probiotic-derived isolates in a tertiary care center in Hungary / Andrea Harmath, Bálint Németh, István Pócsi, László Majoros, Valter Pfliegler, Renátó Kovács
Dátum:2026
ISSN:2165-0497
Megjegyzések:Saccharomyces cerevisiae is traditionally considered a non-pathogenic microbe; however, recent evidence has established its role as an opportunistic fungal pathogen. The probiotic subtype, var. "boulardii," is widely administered for the prevention and treatment of gastrointestinal disorders. Here, we evaluated 46 clinical S. cerevisiae isolates obtained from a tertiary care center in Hungary. Conventional species identification was complemented by multiplex PCR fingerprinting and phylogenomic assignment within a global genomic framework. In addition to these approaches, antifungal susceptibility testing, agar invasiveness, and killer activity assays were performed to provide a comprehensive characterization. Of the isolates, 22 (48%) clustered within the probiotic subclade, predominantly originating from respiratory tract samples, while 15 (33%) corresponded to clades associated with commercial baker's yeasts. Probiotic-derived isolates were significantly associated with pediatric patients (<18 years; OR = 15.92, P = 0.004) and with prior probiotic administration (S. "boulardii": OR = 11.0, P = 0.003). Caspofungin and anidulafungin retained consistent in vitro activity, whereas amphotericin B and micafungin exhibited broader MIC distributions. Sporadic isolates from wine and ale yeast clades highlighted the heterogeneous origins of clinical S. cerevisiae. Regardless of their phylogenomic relationships, isolates generally lacked invasiveness or complex colony morphologies that are often regarded as potential virulence traits. This study demonstrates that clinical S. cerevisiae isolates frequently derive from probiotic or industrial lineages, with distinct patient associations. Integrating phylogenomics, antifungal profiling, and clinical parameters, all obtained in a single location, provides critical insights into the epidemiology, pathogenic potential, and therapeutic management of this potentially emerging fungal pathogen.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
probiotic
Saccharomyces boulardii
susceptibility
phylogenomics
yeast
invasivity
Megjelenés:Microbiology Spectrum. - 14 : 1 (2026), p. 1-16. -
További szerzők:Németh Bálint (1999-) (mikrobiológia) Pócsi István (1961-) (vegyész) Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Pfliegler Valter Péter (1986-) (molekuláris biológus) Kovács Renátó László (1987-) (molekuláris biológus)
Internet cím:Szerző által megadott URL
DOI
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4.

001-es BibID:BIBFORM118634
035-os BibID:(WOS)001180011600002 (Scopus)85189336394
Első szerző:Jakab Ágnes (biológus)
Cím:Comparative transcriptional analysis of Candida auris biofilms following farnesol and tyrosol treatment / Ágnes Jakab, Fruzsina Kovács, Noémi Balla, Csaba Nagy-Köteles, Ágota Ragyák, Fruzsina Nagy, Andrew Borman, László Majoros, Renátó Kovács
Dátum:2024
ISSN:2165-0497
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Microbiology Spectrum. - 12 : 4 (2024), p. 1-12. -
További szerzők:Kovács Fruzsina (1995-) (molekuláris biológus, biotechnológus) Balla Noémi (1998-) (mikrobiológus) Nagy-Köteles Csaba Dienes Ágota Zsófia (1997-) (vegyészmérnök) Nagy Fruzsina (1991-) (molekuláris biológus) Borman, Andrew M. Majoros László (1966-) (szakorvos, klinikai mikrobiológus) Kovács Renátó László (1987-) (molekuláris biológus)
Pályázati támogatás:FK138462
OTKA
BO/00127/21/8
MTA
ÚNKP-22-5-DE-417
Egyéb
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
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5.

001-es BibID:BIBFORM114495
035-os BibID:(WoS)001107303900013 (Scopus)85175710886
Első szerző:Vig Ildikó (biológus, molekuláris biológus)
Cím:Functional characterization of genes encoding cadmium pumping P1B-type ATPases in Aspergillus fumigatus and Aspergillus nidulans / Ildikó Vig, Zsigmond Benkő, Barnabás Cs. Gila, Zoltán Palczert, Ágnes Jakab, Fruzsina Nagy, Márton Miskei, Mi-Kyung Lee, Jae-Hyuk Yu, István Pócsi, Tamás Emri
Dátum:2023
ISSN:2165-0497
Megjegyzések:Several P-1B-type ATPases are important Cd2+/Cu2+ pumps in Aspergillus species, and they are tightly associated with the heavy metal stress tolerance of these ascomycetous fungi. To better understand the roles of the two P-1B-type ATPases, Aspergillus nidulans CrpA Cd2+/Cu2+ pump (orthologue of the Candida albicans Crp1 Cd2+/Cu2+ pump) and Aspergillus fumigatus PcaA Cd2+ pump (orthologue of the Saccharomyces cerevisiae Pca1 Cd2+ pump), we have generated individual mutants and characterized their heavy metal susceptibilities. The deletion of CrpA in A. nidulans has led to the increased sensitivity of the fungus to stresses induced by Zn2+, Fe2+, or the combination of oxidative-stress-inducing menadione sodium bisulfite and Fe3+. Heterologous expression of A. fumigatus PcaA in the S. cerevisiae pca1 deletion mutant has resulted in enhanced tolerance of the yeast to stresses elicited by Cd(2+)or Zn2+ but not by Fe2+/Fe3+ or Cu2+. Mammalian host immune defense can attack microbes by secreting Zn2+ or Cu2+, and the oxidative stress induced by host immune systems can also disturb metal (Cu2+, Fe2+, and Zn2+) homeostasis in microbes. In summary, PcaA and CrpA can protect fungal cells from these complex stresses that contribute to the virulence of the pathogenic Aspergillus species. Moreover, due to their presence on the fungal cell surface, these P-1B-type ATPases may serve as a novel drug target in the future.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Aspergillus fumigatus
Aspergillus nidulans
PcaA cadmium pump
CrpA copper pump
heavy metal homeostasis
P1B-type ATPase
virulence
zinc toxicity
Megjelenés:Microbiology Spectrum. - 11 : 5 (2023), p. 1-14 . -
További szerzők:Benkő Zsigmond (1961-) (molekuláris biológus, genetikus) Gila Csaba Barnabás (1994-) (biotechnológus, biomérnök) Palczert Zoltán Jakab Ágnes (1987-) (biológus) Nagy Fruzsina Miskei Márton (1978-) (molekuláris biológus, genetikus) Lee Mi-Kyung Yu Jae-Hyuk Pócsi István (1961-) (vegyész) Emri Tamás (1969-) (biológus)
Pályázati támogatás:K131767
Egyéb
NN125671
Egyéb
EFOP-3.6.1-16-2016-00022
EFOP
TKP2021-EGA-20
Egyéb
ÚNKP-21-3
Egyéb
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DOI
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