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001-es BibID:	BIBFORM119632
035-os BibID:	(Scopus)85186383343
Első szerző:	De Smedt, Julie
Cím:	Clinical and genetic determinants of vitamin D receptor expression in cutaneous melanoma patients / Julie De Smedt, Claudia Aura, Sofie Van Kelst, Laudine Janssen, Vivien Marasigan, Veerle Boecxstaens, Marguerite Stas, Kris Bogaerts, Ann Belmans, Isabelle Cleynen, Dirk Vanderschueren, Katleen Vandenberghe, Oliver Bechter, Arjen Nikkels, Tinne Strobbe, Gabriella Emri, Dieter Lambrechts, Marjan Garmyn
Dátum:	2024
ISSN:	0960-8931
Megjegyzések:	Decrease of vitamin D receptor (VDR) expression is observed in melanocytic naevi and melanoma compared to normal skin. Little is known about factors influencing VDR expression in cutaneous melanoma (CM). We investigated the correlation of VDR expression in CM with 25-hydroxy vitamin D (25OHD) levels, demographic/clinical parameters, genetic variants of VDR and pathology of the primary tumor. Demographic/clinical parameters were recorded in 407 prospectively recruited CM patients of a multi-center controlled study (ViDMe trial). We determined VDR expression both in the nucleus and in the cytoplasm by semi-quantitative assessment in CM tissue using histochemistry in 279 patients, expressed in percentages and histoscore (H-score). Genomic DNA from 332 patients was extracted to genotype thirteen VDR single nucleotide polymorphisms (SNPs) using TaqMan. VDR expression in CM tissue from 279 patients was correlated with clinical/ demographic parameters and 25OHD levels (univariable and multivariable analysis), VDR SNPs (univariable analysis) and pathology parameters of primary CM tissue (univariable analysis). Cytoplasmic VDR expression was increased in patients who stated to have a high sun exposure during their life compared to patients with low sun exposure (pH-score,univariable: 0.001, pH-score,multivariable: 0.004). The A allele of the genetic VDR polymorphism Fok1 was associated with a higher expression of the VDR in the cytoplasm (pcytoplasmic, univariable: 0.001 and pH-score, univariable: 0.02). In the primary tumor, presence of mitosis (pnucleus,%, univariable: 0.002) and perineural invasion (pnucleus,%,univariable: 0.03) were significantly associated with low nuclear VDR expression.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk cutaneous melanoma vitamin D receptor vitamin D receptor polymorphisms
Megjelenés:	Melanoma Research. - 34 : 2 (2024), p. 125-133. -
További szerzők:	Aura, Claudia Van Kelst, Sofie Janssen, Laudine Marasigan, Vivien Boecxstaens, Veerle Stas, Marguerite Bogaerts, Kris Belmans, Ann Cleynen, Isabelle Vanderschueren, Dirk Vandenberghe, Katleen Bechter, Oliver Nikkels, Arjen Strobbe, Tinne Emri Gabriella (1972-) (bőrgyógyász, allergológus, onkológus) Lambrechts, Dieter (1976-) (genetikus) Garmyn, Marjan
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001-es BibID:	BIBFORM049631
Első szerző:	Lázár Viktória (molekuláris biológus)
Cím:	Marked genetic differences between BRAF and NRAS mutated primary melanomas as revealed by array comparative genomic hybridization / Viktória Lázár, Szilvia Ecsedi, Laura Vízkeleti, Zsuzsa Rákosy, Gábor Boross, Balázs Szappanos, Ágnes Bégány, Gabriella Emri, Róza Ádány, Margit Balázs
Dátum:	2012
ISSN:	0960-8931
Megjegyzések:	Somatic mutations of BRAF and NRAS oncogenes are thought to be among the first steps in melanoma initiation, but these mutations alone are insufficient to cause tumor progression. Our group studied the distinct genomic imbalances of primary melanomas harboring different BRAF or NRAS genotypes. We also aimed to highlight regions of change commonly seen together in different melanoma subgroups. Array comparative genomic hybridization was performed to assess copy number changes in 47 primary melanomas. BRAF and NRAS were screened for mutations by melting curve analysis. Reverse transcription PCR and fluorescence in-situ hybridization were performed to confirm the array comparative genomic hybridization results. Pairwise comparisons revealed distinct genomic profiles between melanomas harboring different mutations. Primary melanomas with the BRAF mutation exhibited more frequent losses on 10q23-q26 and gains on chromosome 7 and 1q23-q25 compared with melanomas with the NRAS mutation. Loss on the 11q23-q25 sequence was found mainly in conjunction with the NRAS mutation. Primary melanomas without the BRAF or the NRAS mutation showed frequent alterations in chromosomes 17 and 4. Correlation analysis revealed chromosomal alterations that coexist more often in these tumor subgroups. To find classifiers for BRAF mutation, random forest analysis was used. Fifteen candidates emerged with 87% prediction accuracy. Signaling interactions between the EGF/MAPK-JAK pathways were observed to be extensively altered in melanomas with the BRAF mutation. We found marked differences in the genetic pattern of the BRAF and NRAS mutated melanoma subgroups that might suggest that these mutations contribute to malignant melanoma in conjunction with distinct cooperating oncogenic events.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban array comparative genomic hybridization BRAF mutation Egészség- és Környezettudomány NRAS mutation primary melanoma signaling pathway Egészség- és Környezettudomány
Megjelenés:	Melanoma Research. - 22 : 3 (2012), p. 202-214. -
További szerzők:	Ecsedi Szilvia (1982-) (molekuláris biológus, genetikus) Vízkeleti Laura (1984-) (molekuláris biológus, genetikus) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Boross Gábor Szappanos Balázs Bégány Ágnes (1954-2011) (bőrgyógyász, kozmetológus, klinikai onkológus) Emri Gabriella (1972-) (bőrgyógyász, allergológus, onkológus) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Daganatprogresszió genetikai markerei
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001-es BibID:	BIBFORM039768
035-os BibID:	PMID:12140379
Első szerző:	Szincsák Nóra
Cím:	Cimetidine and a tamoxifen derivate reduce tumour formation in SCID mice xenotransplanted with a human melanoma cell line / N. Szincsák, H. Hegyesi, J. Hunyadi, G. Martin, E. Lázár-Molnár, P. Kovács, E. Rivera, A. Falus, I. Juhász
Dátum:	2002
ISSN:	0960-8931
Megjegyzések:	Histamine is produced by many cells expressing histidine decarboxylase (HDC), the enzyme responsible for the synthesis of histamine. Since melanoma cells and tissue contain relatively large amounts of histamine, the functional significance of histamine was examined using specific antihistamines in vitro and in vivo in the human melanoma cell line HT168 and severe combined immunodeficiency (SCID) mice. It was shown that the H2 receptor antagonist cimetidine when combined with N, N-diethyl-2-[4-(phenylmethyl)phenoxy]-ethanamine-HCl (DPPE), a tamoxifen derivate, inhibits the proliferation of HT168 cells. Furthermore, it is suggested that there is a factor(s) that interferes with the exponential growth of HT168 cells xenografted to immunodeficient mice, and cimetidine and DPPE together significantly influence this factor(s). This combination of antihistamines also increases the survival of human melanoma-grafted mice. These changes are accompanied by enhanced infiltration of interferon-gamma- producing mouse macrophages into the tumour tissue. These findings suggest that two different mechanisms are probably acting concordantly: direct inhibition of tumour cell proliferation by the H2 receptor antagonists, and activation of the local immune response characterized by interferon-gamma production. These findings may help to elucidate the possibility of a rationally designed antihistamine strategy in melanoma therapy.
Tárgyszavak:	Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Melanoma Research. - 12 : 3 (2002), p. 231-240. -
További szerzők:	Hegyesi Hargita Martin, G. Lázár-Molnár Eszter Kovács Péter (1939-) (farmakológus) Rivera, E. Falus András Juhász István (1956-) (bőrgyógyász, bőrsebész, kozmetológus, klinikai onkológus) Hunyadi János (1943-) (bőrgyógyász, kozmetológus, allergológus)
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