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001-es BibID:	BIBFORM106794
035-os BibID:	(scopus)85141755492 (wos)000881482500001
Első szerző:	Farsang Róbert
Cím:	Ultrahigh-Sensitivity Capillary Electrophoresis Analysis of Trace Amounts of Nitrate and Nitrite in Environmental Water Samples / Farsang Robert, Kovacs Zsofia, Jarvas Gabor, Guttman Andras
Dátum:	2022
ISSN:	2297-8739
Megjegyzések:	The role of nitrite (NO2?) and nitrate (NO3?) is essential in the global nitrogen cycle. Monitoring their concentration in environmental and industrial aqueous samples, surface water, soil, food and agricultural products are of high importance. Especially, the effect of anthropogenic emission, i.e., intensified agriculture is essential due to the overuse of nitrogen, phosphorus and potassium fertilizers. The most widely utilized methods for nitrate and nitrite determination are colorimetry, potentiometry, UV absorption and liquid chromatography. Among them, UV spectroscopy is the most frequently used technique due to the fact of its versatility and simplicity. However, there are industrial and academic needs to develop new methods to overcome some drawbacks of the currently used techniques such as an inadequate limit of detection and potential interferences with organic compounds in the sample. In this paper, we report on the development of a new analytical method based on capillary electrophoresis separation with high-sensitivity UV detection, capable of measuring trace concentrations of nitrite and nitrate well below the current limits of UV spectroscopy methods. During the development process special attention was paid to practical aspects, i.e., the method was tested to quantify nitrate and nitrite in various surface water samples.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Separations. - 9 : 11 (2022), p. 1-7. -
További szerzők:	Kovács Zsófia Járvás Gábor (1982-) (vegyészmérnök) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	RRF 2.3.1-21-2022-00008 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM135985
035-os BibID:	(scopus)105006755129 (wos)001496870300001
Első szerző:	Szerényi Dóra
Cím:	Development of a High-Performance Immunoaffinity Separation Technique for Rare Cell Capture / Szerenyi Dora, Stolk Paul, Tovari Jozsef, Takacs Laszlo, Guttman Andras
Dátum:	2025
ISSN:	2297-8739
Megjegyzések:	Circulating tumor cell enrichment and enumeration are advancing early detection of cancer, monitoring of therapy response, and even next-generation therapies. Efficiently capturing rare cells from complex biological fluids is essential in both diagnostic and therapeutic applications. EpCAM-positive tumor cells are specifically captured by utilizing covalently immobilized anti-EpCAM monoclonal antibodies onto the surface of chemically modified glass microbeads. To maximize the capture efficiency, bead geometry, immobilization conditions, flow rate, and anticoagulant dosage were systematically optimized. An in vitro flow-capture system was designed and used to evaluate the capture efficiency of the proposed technology by utilizing HTC116 colon cancer cell-spiked model media. The effect of substrate surface pretreatment was characterized by goniometry, while the capture performance was monitored by flow cytometry and fluorescent microscopy. The specific capture ability of the bioactive microbead substrate reached over 130,000 cells in the laboratory-scale cartridge (V(cartridge) = 2.6 cm3; m(bead) = 4 g). This capture efficiency suggests a promising rare-cell capture utilization of the proposed technology and may be used for research, diagnostic, and therapeutic purposes. In this paper, we reported on the development and feasibility test of a high-performance bioactive glass-microbead cell capture substrate. Due to the relevance and novelty of the reported results, with further development, the versatile platform technology presented could be readily implemented to capture tumor cells from complex biological samples and represent an additional complementary tool to existing cancer diagnostics and therapies.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk CTC immunoaffinity microbeads cell capture diagnostics personalized medicine
Megjelenés:	Separations. - 12 : 5 (2025), p. 1-18. -
További szerzők:	Stolk, Paul Tóvári József Takács László (1955-) (genombiológus, immunológus) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	PTP2025 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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