Találati lista | Tudóstér

001-es BibID:	BIBFORM127812
035-os BibID:	(Scopus)85208330047 (WoS)001381334300001
Első szerző:	Geyer Nikolett
Cím:	Ca2+ signaling of pancreatic acinar cells in malignant hyperthermia susceptibility / Nikolett Geyer, Gyula Diszházi, Zsuzsanna É. Magyar, Beatrix Dienes, Réka Csáki, Péter Enyedi, Tamara Madácsy, József Maléth, János Almássy
Dátum:	2024
ISSN:	1424-3903
Megjegyzések:	Background: Malignant hyperthermia susceptibility (MHS) and acute pancreatitis (AP) share a common cellular pathomechanism that is Ca2+-overload of the muscle fiber and the pancreatic acinar cell (PAC). In the muscle, gain-of-function mutations of the ryanodine receptor (RyR1) make the Ca2+-release mechanism hypersensitive to certain ligands, including Ca2+, volatile anaesthetics and succinylcholine, creating a medical emergency when the patient is exposed to these drugs. As RyR1 was shown to contribute to Ca2+-overload in PAC, we presumed that pancreata of MHS individuals are more prone to AP. Accordingly, a recent case study reported coincidence of MHS with recurrent AP, indicating a pathological link between the two diseases. Methods: We tested if MHS poses a risk for AP in mice carrying the Y522S MHS mutation. Fluorescent Ca2+ imaging was performed in PACs. Conventional histopathological analysis and plazma amylase measurement was performed using a cerulein-induced pancreatitis mouse model. Results: The intracellular Ca2+-signals of PACs from MHS mice were slightly bigger then in wild type when stimulated with 0.2 and 2 ?M carbachol (cch) or with 1 and 5 mM bile acid (taurocholic acid). Store-operated-Ca2+-entry was also higher in PACs from MHS mice. Nevertheless, histopathological analysis and plasma amylase levels did not indicate more severe AP in MHS. Conclusions: These results suggest that the Y522S RyR1 mutation alter the Ca2+-homeostasis in PACs, but not as much as to cause or aggravate AP.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Acute pancreatitis Calcium signaling Malignant hyperthermia Pancreatic acinar cells Ryanodine receptor
Megjelenés:	Pancreatology. - 24 : 8 (2024), p. 1257-1264. -
További szerzők:	Diszházi Gyula (1992-) (gyógyszerész) Magyar Zsuzsanna Édua (1993-) (molekuláris biológus) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Csáki Réka Enyedi Péter Madácsy Tamara Maléth József Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM076264
035-os BibID:	(WoS)000435065400002 (Scopus)85048475484 (PubMed)29589836
Első szerző:	Vincze János (biofizikus)
Cím:	Laser induced calcium oscillations in fluorescent calcium imaging / János Vincze, Nikolett Geyer, Gyula Diszházi, László Csernoch, Tamás Bíró, István Jóna, Beatrix Dienes, János Almássy
Dátum:	2018
Megjegyzések:	Phototoxicity is the most common problem investigators may encounter when performing live cell imaging. It develops due to excess laser exposure of cells loaded with fluorophores and can lead to often overlooked but significant artifacts, such as massive increase of intracellular Ca2+ concentration, which would make data interpretation problematic. Because information about laserand dye-related changes in cytoplasmic calcium concentration is very limited, we aimed to describe this phenomenon to help investigators using laser scanning confocal microscopy in a non-invasive way. Therefore, in the present study we evaluated fluorescent fluctuations, which evolved in Fluo3/4/8 loaded mouse pancreatic acinar cells during very low intensity laser excitation. We demonstrate that after standard loading procedure (2 ?M Fluo-3/4/8-AM, 30 min @ room temperature), applying 488 nm laser at as low as ca. 10 ?W incident laser power (0.18 ?W/?m2) at 1 Hz caused repetitive, 2-3 fold elevations of the resting intracellular fluorescence. The first latency and the pattern of the fluorescence fluctuations were laser power dependent and were related to Ca2+-release from intracellular stores, as they were abolished by BAPTA-AM treatment in Ca2+- free medium, but were not diminished by the ROS scavenger DMPO. Worryingly enough, the qualitative and quantitative features of the Ca2+-waves were practically indistinguishable from the responses evoked by secretagogue stimulation. Since using similar imaging conditions, a number of other cell types were reported to display spontaneous Ca2+ oscillations, we propose strategies to distinguish the real signals from artefacts.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	General physiology and biophysics. - 37 : 3 (2018), p. 253-261. -
További szerzők:	Geyer Nikolett Diszházi Gyula (1992-) (gyógyszerész) Csernoch László (1961-) (élettanász) Bíró Tamás (1968-) (élettanász) Jóna István (1948-) (élettanász, fizikus) Dienes Beatrix (1972-) (élettanász, molekuláris biológus) Almássy János (1981-) (élettanász, biológus, angol-magyar szakfordító)
Pályázati támogatás:	PD 112199 OTKA TÁMOP-4.2.4.A/2-11/1-2012-0001 TÁMOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: