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001-es BibID:	BIBFORM122718
035-os BibID:	(Scopus)85193926706 (WOS)001229202800001
Első szerző:	Dócs Klaudia (orvos)
Cím:	Reactive spinal glia convert 2-AG to prostaglandins to drive aberrant astroglial calcium signaling / Klaudia Dócs, Anita Balázs, Ildikó Papp, Peter Szücs, Zoltán Hegyi
Dátum:	2024
ISSN:	1662-5102
Megjegyzések:	The endogenous cannabinoid 2-arachidonoylglycerol (2-AG) influences neurotransmission in the central nervous system mainly by activating type 1 cannabinoid receptor (CB1). Following its release, 2-AG is broken down by hydrolases to yield arachidonic acid, which may subsequently be metabolized by cyclooxygenase-2 (COX-2). COX-2 converts arachidonic acid and also 2-AG into prostanoids, well-known inflammatory and pro-nociceptive mediators. Here, using immunohistochemical and biochemical methods and pharmacological manipulations, we found that reactive spinal astrocytes and microglia increase the expression of COX-2 and the production of prostaglandin E2 when exposed to 2-AG. Both 2-AG and PGE2 evoke calcium transients in spinal astrocytes, but PGE2 showed 30% more efficacy and 55 times more potency than 2-AG. Unstimulated spinal dorsal horn astrocytes responded to 2-AG with calcium transients mainly through the activation of CB1. 2-AG induced exaggerated calcium transients in reactive astrocytes, but this increase in the frequency and area under the curve of calcium signals was only partially dependent on CB1. Instead, aberrant calcium transients were almost completely abolished by COX-2 inhibition. Our results suggest that both reactive spinal astrocytes and microglia perform an endocannabinoid-prostanoid switch to produce PGE2 at the expense of 2-AG. PGE2 in turn is responsible for the induction of aberrant astroglial calcium signals which, together with PGE2 production may play role in the development and maintenance of spinal neuroinflammation-associated disturbances such as central sensitization.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk 2-AG astrocyte calcium signaling cannabinoid CB1 COX-2 prostaglandin reactive astrocyte
Megjelenés:	Frontiers in Cellular Neuroscience. - 18 (2024), p. 1-17. -
További szerzők:	Balázs Anita (1984-) (molekuláris biológus) Papp Ildikó (1976-) (biológus) Szűcs Péter (1974-) (kutatóorvos) Hegyi Zoltán (1983-) (molekuláris biológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM109585
035-os BibID:	(Scopus)85150718609 (WoS)000951373400001
Első szerző:	Ducza László (molekuláris biológus)
Cím:	Neuronal P2X4 receptor may contribute to peripheral inflammatory pain in rat spinal dorsal horn / Ducza László, Gajtkó Andrea, Hegedűs Krisztina, Bakk Erzsébet, Kis Gréta, Gaál Botond, Takács Roland, Szücs Péter, Matesz Klára, Holló Krisztina
Dátum:	2023
ISSN:	1662-5099
Megjegyzések:	Objective: Intense inflammation may result in pain, which manifests as spinal central sensitization. There is growing evidence that purinergic signaling plays a pivotal role in the orchestration of pain processing. Over the last decade the ionotropic P2X purino receptor 4 (P2X4) got into spotlight in neuropathic disorders, however its precise spinal expression was scantily characterized during inflammatory pain. Thus, we intended to analyze the receptor distribution within spinal dorsal horn and lumbar dorsal root ganglia (DRG) of rats suffering in inflammatory pain induced by complete Freund adjuvant (CFA). Methods: CFA-induced peripheral inflammation was validated by mechanical and thermal behavioral tests. In order to ensure about the putative alteration of spinal P2X4 receptor gene expression qPCR reactions were designed, followed by immunoperoxidase and Western blot experiments to assess changes at a protein level. Colocalization of P2X4 with neuronal and glial markers was investigated by double immunofluorescent labelings, which were subsequently analyzed with IMARIS software. Transmission electronmicroscopy was applied to study the ultrastructural localization of the receptor. Concurrently, in lumbar DRG cells similar methodology has been carried out to complete our observations. Results: The figures of mechanical and thermal behavioral tests proved the establishment of CFA-induced inflammatory pain. We observed significant enhancement of P2X4 transcript level within the spinal dorsal horn 3?days upon CFA administration. Elevation of P2X4 immunoreactivity within Rexed lamina I-II of the spinal gray matter was synchronous with mRNA expression, and confirmed by protein blotting. According to IMARIS analysis the robust protein increase was mainly detected on primary afferent axonterminals and GFAP-labelled astrocyte membrane compartments, but not on postsynaptic dendrites was also validated ultrastructurally within the spinal dorsal horn. Furthermore, lumbar DRG analysis demonstrated that peptidergic and non-peptidergic nociceptive subsets of ganglia cells were also abundantly positive for P2X4 receptor in CFA model. Conclusion: Here we provide novel evidence about involvement of neuronal and glial P2X4 receptor in the establishment of inflammatory pain.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk inflammatory pain spinal dorsal horn P2X4 receptor central sensitization primary afferents glial cells dorsal root ganglia
Megjelenés:	Frontiers in Molecular Neuroscience. - 16 (2023), p. 1-16. -
További szerzők:	Gajtkó Andrea (1989-) (molekuláris biológus) Hegedűs Krisztina Bakk Erzsébet Kis Gréta (1979-) (molekuláris biológus) Gaál Botond Ágoston (1982-) (anatómus, neurobiológus) Takács Roland Ádám (1985-) (molekuláris biológus, biokémikus) Szűcs Péter (1974-) (kutatóorvos) Matesz Klára (1949-) (anatómus, neurobiológus) Holló Krisztina (1967-) (vegyész)
Pályázati támogatás:	KTIA_NAP_13-2-2014-0005 Egyéb 2017-1.2.1-NKP-2017-00002 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM100951
035-os BibID:	(Scopus)85127948706 (wos)000779086500001
Első szerző:	Papp Tamás (orvos)
Cím:	Ultrasound Used for Diagnostic Imaging Facilitates Dendritic Branching of Developing Neurons in the Mouse Cortex / Tamas Papp, Zsuzsanna Ferenczi, Bernadette Szilagyi, Matyas Petro, Angelika Varga, Eva Kókai, Ervin Berenyi, Gabor Olah, Gabor Halmos, Peter Szucs, Zoltan Meszar
Dátum:	2022
ISSN:	1662-453X
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Frontiers in Neuroscience. - 16 (2022), p. 1-13. -
További szerzők:	Winkler-Ferenczi Zsuzsanna Szilágyi Bernadett (1993-) Petró Mátyás (1985-) (radiológus) Varga Angelika (1977-) (biológus) Kókai Éva (1989-) (molekuláris biológus) Berényi Ervin (1964-) (radiológus) Oláh Gábor (1987-) (gyógyszerész) Halmos Gábor (1962-) (gyógyszerész, receptorfarmakológus, experimentális onkológus) Szűcs Péter (1974-) (kutatóorvos) Mészár Zoltán Mihály (1977-) (agrármérnök, biotechnológus)
Pályázati támogatás:	TKP2021-EGA-20 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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