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1.

001-es BibID:BIBFORM103405
035-os BibID:(WoS)000858038100001 (Scopus)85138606448
Első szerző:Beke-Varga Alexandra Edit (molekuláris biológus)
Cím:Suppressing the PI3K/AKT Pathway by miR-30d-5p Mimic Sensitizes Ovarian Cancer Cells to Cell Death Induced by High-Dose Estrogen / Varga Alexandra, Márton Éva, Markovics Arnold, Penyige András, Balogh István, Nagy Bálint, Szilágyi Melinda
Dátum:2022
ISSN:2227-9059
Megjegyzések:MicroRNAs are short non-coding RNA molecules that are involved in tumor development and are considered to be promising candidates in cancer therapy. Here, we studied the role of miR-30s in the pathophysiology of ovarian cancer. According to our results miR-30a-5p, miR-30d-5p, and miR-30e-5p were overexpressed in the estrogen receptor alpha (ER alpha)-expressing PEO1 cell line compared to A2780 that lacks this receptor. Furthermore, the expression of miR-30a-5p, miR-30d-5p, and miR-30e-5p were induced in response to high-dose estrogen treatment in PEO1 where intensive cell death was observed according to the induction of apoptosis and autophagy. Lacking or blocking ER alpha function reduced tolerance to high-dose estrogen that suggests the importance of ER alpha-mediated estrogen response in the maintenance of proliferation. MiR-30d-5p mimic reduced cell proliferation in both A2780 and PEO1. Furthermore, it decreased the tolerance of PEO1 cells to high-dose estrogen by blocking the ER alpha-mediated estrogen response. This was accompanied by decreased SOX4 expression that is thought to be involved in the regulation of the PI3K/AKT pathway. Blocking this pathway by AZD8835 led to the same results. MiR-30d-5p or AZD8835 sensitized PEO1 cells to tamoxifen. We suggest that miR-30d-5p might be a promising candidate in the therapy of ovarian cancer.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
miR-30
ovarian cancer
estrogen receptor
high-dose estrogen
cell proliferation
cell death
sox4
PI3K/AKT
cancer therapy
tamoxifen
Megjelenés:Biomedicines. - 10 : 9 (2022), p. 1-19. -
További szerzők:Márton Éva (1992-) (biológus) Markovics Arnold (1988-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:EFOP-3.6.3-VEKOP-16-2017-00009
EFOP
138021
OTKA
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2.

001-es BibID:BIBFORM129151
035-os BibID:(Scopus)105001011774 (WoS)001452091200001
Első szerző:Géczi Dóra (biotechnológus)
Cím:Identification of Deregulated miRNAs and mRNAs Involved in Tumorigenesis and Detection of Glioblastoma Patients Applying Next-Generation RNA Sequencing / Dóra Géczi, Álmos Klekner, István Balogh, András Penyige, Melinda Szilágyi, József Virga, Andrea Bakó, Bálint Nagy, Bernadett Torner, Zsuzsanna Birkó
Dátum:2025
ISSN:1424-8247
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Pharmaceuticals. - 18 : 3 (2025), p. 1-29. -
További szerzők:Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Virga József (1989-) Bakó Andrea (1979-) (orvos) Nagy Bálint (1956-) (molekuláris genetikus) Torner Bernadett (1997-) (molekuláris biológus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002 of the "National Brain Research Program NAP 2.0
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Intézményi repozitóriumban (DEA) tárolt változat
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3.

001-es BibID:BIBFORM127700
035-os BibID:(WoS)001429655400001
Első szerző:Márton Éva (biológus)
Cím:Non-Coding RNAs in Cancer : structure, Function, and Clinical Application / Éva Márton, Alexandra Varga, Dóra Domoszlai, Gergely Buglyó, Anita Balázs, András Penyige, István Balogh, Bálint Nagy, Melinda Szilágyi
Dátum:2025
ISSN:2072-6694
Megjegyzések:We are on the brink of a paradigm shift in both theoretical and clinical oncology. Genomic and transcriptomic profiling, alongside personalized approaches that account for individual patient variability, are increasingly shaping discourse. Discussions on the future of personalized cancer medicine are mainly dominated by the potential of non-coding RNAs (ncRNAs), which play a prominent role in cancer progression and metastasis formation by regulating the expression of oncogenic or tumor suppressor proteins at transcriptional and post-transcriptional levels; furthermore, their cell-free counterparts might be involved in intercellular communication. Non-coding RNAs are considered to be promising biomarker candidates for early diagnosis of cancer as well as potential therapeutic agents. This review aims to provide clarity amidst the vast body of literature by focusing on diverse species of ncRNAs, exploring the structure, origin, function, and potential clinical applications of miRNAs, siRNAs, lncRNAs, circRNAs, snRNAs, snoRNAs, eRNAs, paRNAs, YRNAs, vtRNAs, and piRNAs. We discuss molecular methods used for their detection or functional studies both in vitro and in vivo. We also address the challenges that must be overcome to enter a new era of cancer diagnosis and therapy that will reshape the future of oncology.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
cancer
cancer diagnostics
cancer therapy
RNA
RNA detection
miRNA
lncRNA
circRNA
snRNA
snoRNA
Megjelenés:Cancers. - 17 : 4 (2025), p. 1-41. -
További szerzők:Beke-Varga Alexandra Edit (1994-) (molekuláris biológus) Domoszlai Dóra (1999-) (molekuláris biológus) Buglyó Gergely (1980-) (genetikus) Balázs Anita (1984-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:OTKA-138021
OTKA
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Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM108635
035-os BibID:(Scopus)85148964475
Első szerző:Márton Éva (biológus)
Cím:Comparative Analysis of Transcriptomic Changes including mRNA and microRNA Expression Induced by the Xenoestrogens Zearalenone and Bisphenol A in Human Ovarian Cells / Éva Márton, Alexandra Varga, András Penyige, Zsuzsanna Birkó, István Balogh, Bálint Nagy, Melinda Szilágyi
Dátum:2023
ISSN:2072-6651
Megjegyzések:Xenoestrogens are natural or synthetic compounds that mimic the effect of endogenous estrogens and might cause cancer. We aimed to compare the global transcriptomic response to zearalenone (ZEA; mycotoxin) and bisphenol A (BPA; plastic additive) with the effect of physiological estradiol (E2) in the PEO1 human ovarian cell line by mRNA and microRNA sequencing. Estrogen exposure induced remarkable transcriptomic changes: 308, 288 and 63 genes were upregulated (log2FC > 1); 292, 260 and 45 genes were downregulated (log2FC < ?1) in response to E2 (10 nM), ZEA (10 nM) and BPA (100 nM), respectively. Furthermore, the expression of 13, 11 and 10 miRNAs changed significantly (log2FC > 1, or log2FC < ?1) after exposure to E2, ZEA and BPA, respectively. Functional enrichment analysis of the significantly differentially expressed genes and miRNAs revealed several pathways related to the regulation of cell proliferation and migration. The effect of E2 and ZEA was highly comparable: 407 genes were coregulated by these molecules. We could identify 83 genes that were regulated by all three treatments that might have a significant role in the estrogen response of ovarian cells. Furthermore, the downregulation of several miRNAs (miR-501- 5p, let-7a-2-3p, miR-26a-2-3p, miR-197-5p and miR-582-3p) was confirmed by qPCR, which might support the proliferative effect of estrogens in ovarian cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
xenoösztrogén
zearalenon
mikotoxin
biszfenol A
petefészekrák
transzkriptomika
mikroRNS
RNS szekvenálás
Megjelenés:Toxins. - 15 : 2 (2023), p. 1-22. -
További szerzők:Beke-Varga Alexandra Edit (1994-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:FK138021
OTKA
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Intézményi repozitóriumban (DEA) tárolt változat
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5.

001-es BibID:BIBFORM136487
035-os BibID:(scopus)105032156153 (wos)001707066300001
Első szerző:Torner Bernadett (molekuláris biológus)
Cím:The role of miRNAs in the development of brain metastases originating from lung adenocarcinoma / Torner Bernadett, Klekner Álmos, Balogh István, Penyige András, Géczi Dóra, Gáspár Tekla, Geszti Gréta, Birkó Zsuzsanna
Dátum:2026
ISSN:1664-8021
Megjegyzések:Introduction: Brain metastases (BMs) represent most malignant lesions of the central nervous system. Lung cancer-particularly lung adenocarcinoma (LUAD, ?25%)-is the most common source of BMs. MicroRNAs (miRNAs) play a crucial role in regulating gene expression, thereby contributing to tumor progression and metastatic spread. Identifying these regulatory molecules may enable a deeper understanding of the mechanisms driving LUAD brain metastasis (LUAD-BM) development and reveal therapeutic targets to prevent or limit disease progression. Methods: Next-generation RNA sequencing (RNA-seq) was performed on six LUAD-BM and six non-tumorous human brain tissue samples to assess miRNA expression profiles. Additionally, RNA-seq data from 20 primary LUAD and 15 normal lung tissue samples were obtained from The Cancer Genome Atlas (TCGA) database. MiRNAs showing the most pronounced alterations in LUAD-BM samples were selected for validation by real time quantitative polymerase chain reaction (RT-qPCR). Results: Analysis of RNA-seq data identified 229 differentially expressed (DE) miRNAs between LUAD-BM and control samples. Functional annotation analysis indicated that these DE miRNAs are key regulators of tumorigenesis and metastasis. Using the Mann-Whitney U test, ten miRNAs were confirmed to differ significantly between LUAD-BM and normal brain tissue. Receiver operating characteristic (ROC) curve analysis demonstrated their diagnostic potential. Among the ten validated miRNAs, miR-200c-3p, miR-146b-5p, and miR-3934-5p showed distinct expression patterns between primary LUAD and LUAD-BM, while miR-10a-5p, miR-210-3p, and miR-130b-3p exhibited stepwise dysregulation along the normal lung-LUAD-LUAD-BM axis, suggesting their involvement in metastatic progression. Conclusion: We identified ten miRNAs that showed preliminary ability to differentiate LUAD-BM from normal brain tissue. These findings indicate possible diagnostic and therapeutic implications. Among these, six miRNAs showed significant expression changes along the normal control-primary LUAD-LUAD-BM axis, highlighting their potential as biomarkers and therapeutic targets in BM development.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
biomarker panel
brain tissue
invasion
lung adenocarcinoma brain metastasis
miRNAs
next-generation sequencing
Megjelenés:Frontiers in Genetics. - 17 (2026), p. 1-17. -
További szerzők:Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Géczi Dóra (1989-) (biotechnológus) Gáspár Tekla Geszti Gréta Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
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Intézményi repozitóriumban (DEA) tárolt változat
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6.

001-es BibID:BIBFORM128116
035-os BibID:(scopus)85218910548 (wos)001429631100001
Első szerző:Torner Bernadett (molekuláris biológus)
Cím:Construction of a miRNA Panel for Differentiating Lung Adenocarcinoma Brain Metastases and Glioblastoma / Torner Bernadett, Géczi Dóra, Klekner Álmos, Balogh István, Penyige András, Birkó Zsuzsanna
Dátum:2025
ISSN:2072-6694
Megjegyzések:Abstract: Background/Objectives: Brain metastases (BM) are the most common type of intracra-nial malignant tumor and are associated with high mortality. More than 50% of BM cases origi-nate from lung cancer, and lung adenocarcinoma (LUAD) is most commonly associated with the development of BM (25%). The differential diagnosis of solitary BM and glioblastoma (GBM) ? one of the most aggressive and fatal malignant brain tumors ? remains a considerable challenge. Given the major role of microRNAs (miRNAs) in regulating gene expression, their clinical po-tential as biomarkers for tumor diagnosis and prognosis offers significant promise. Methods: Next Generation RNA Sequencing (RNA-seq) was used to assess the miRNA expression profiles of 6 LUAD-BM, 6 GBM, and 6 control (non-tumoral brain tissue samples) human brain tissue samples. miRNAs exhibiting the most significant differential expression in LUAD-BM patients in comparison to both control subjects and GBM patients were selected for validation through RT-qPCR. Results: The analysis of RNA-seq data revealed the presence of 229 differentially ex-pressed miRNAs in the comparison between LUAD-BM and control samples and 46 in the com-parison between LU-AD-BM and GBM samples. Eight miRNAs were selected for further analysis, four of which were upregulated and four downregulated, based on the significant differences in their expression levels observed between the LUAD-BM samples and the other two groups, as confirmed with the Mann-Whitney U test. A functional enrichment analysis was also conducted based on a miRNA-centered target analysis performed using the miRNet tool. To assess the di-agnostic potential of these differentially expressed miRNAs, we performed a receiver operating characteristic (ROC) curve analysis. Conclusions: A panel of eight miRNAs was identified in human brain tissue samples, exhibiting high accuracy in distinguishing LUAD-BM from both GBM and control samples.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
lung adenocarcinoma brain metastasis, glioblastoma
miRNAs
Next-Generation Sequencing
brain tissue
biomarker panel
Megjelenés:Cancers. - 17 : 4 (2025), p. 1-23. -
További szerzők:Géczi Dóra (1989-) (biotechnológus) Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002
Egyéb
Bridging Fund of the Faculty of Medicine, University of Debrecen (IB)
Egyéb
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Intézményi repozitóriumban (DEA) tárolt változat
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