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001-es BibID:	BIBFORM129151
035-os BibID:	(Scopus)105001011774 (WoS)001452091200001
Első szerző:	Géczi Dóra (biotechnológus)
Cím:	Identification of Deregulated miRNAs and mRNAs Involved in Tumorigenesis and Detection of Glioblastoma Patients Applying Next-Generation RNA Sequencing / Dóra Géczi, Álmos Klekner, István Balogh, András Penyige, Melinda Szilágyi, József Virga, Andrea Bakó, Bálint Nagy, Bernadett Torner, Zsuzsanna Birkó
Dátum:	2025
ISSN:	1424-8247
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Pharmaceuticals. - 18 : 3 (2025), p. 1-29. -
További szerzők:	Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Virga József (1989-) Bakó Andrea (1979-) (orvos) Nagy Bálint (1956-) (molekuláris genetikus) Torner Bernadett (1997-) (molekuláris biológus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:	2017-1.2.1-NKP-2017-00002 of the "National Brain Research Program NAP 2.0 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM094352
035-os BibID:	(scopus)85105433955 (wos)000662004700001
Első szerző:	Géczi Dóra (biotechnológus)
Cím:	Analysis of Circulating miRNA Profile in Plasma Samples of Glioblastoma Patients / Dóra Géczi, Bálint Nagy, Melinda Szilágyi, András Penyige, Álmos Klekner, Adrienn Jenei, József Virga, Zsuzsanna Birkó
Dátum:	2021
ISSN:	1661-6596 1422-0067
Megjegyzések:	Background: Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis. Treatment options are limited, clinicians lack efficient prognostic and predictive markers. Circulating miRNAs?besides being important regulators of cancer development?may have potential as diagnostic biomarkers of GBM. (2) Methods: In this study, profiling of 798 human miRNAs was performed on blood plasma samples from 6 healthy individuals and 6 patients with GBM, using a NanoString nCounter Analysis System. To validate our results, five miRNAs (hsa-miR-433-3p, hsa-miR-362-3p, hsa-miR-195-5p, hsa-miR-133a-3p, and hsa-miR-29a-3p) were randomly chosen for RT-qPCR detection. (3) Results: In all, 53 miRNAs were significantly differentially expressed in plasma samples of GBM patients when data were filtered for FC 1 and FDR 0.1. Target genes of the top 39 differentially expressed miRNAs were identified, and we carried out functional annotation and pathway enrichment analysis of target genes via GO and KEGG-based tools. General and cortex-specific protein?protein interaction networks were constructed from the target genes of top miRNAs to assess their functional connections. (4) Conclusions: We demonstrated that plasma microRNA profiles are promising diagnostic and prognostic molecular biomarkers that may find an actual application in the clinical practice of GBM, although more studies are needed to validate our results.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk glioblastoma circulating miRNA blood plasma network analysis biomarker NanoString
Megjelenés:	International Journal Of Molecular Sciences. - 22 : 10 (2021), p. 1-20. -
További szerzők:	Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Penyige András (1954-) (molekuláris genetikus) Klekner Álmos (1970-) (idegsebész) Jenei Adrienn (1978-) (biológus, kémikus) Virga József (1989-) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:	2017-1.2.1-NKP-2017-00002 NKP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM136487
035-os BibID:	(scopus)105032156153 (wos)001707066300001
Első szerző:	Torner Bernadett (molekuláris biológus)
Cím:	The role of miRNAs in the development of brain metastases originating from lung adenocarcinoma / Torner Bernadett, Klekner Álmos, Balogh István, Penyige András, Géczi Dóra, Gáspár Tekla, Geszti Gréta, Birkó Zsuzsanna
Dátum:	2026
ISSN:	1664-8021
Megjegyzések:	Introduction: Brain metastases (BMs) represent most malignant lesions of the central nervous system. Lung cancer-particularly lung adenocarcinoma (LUAD, ?25%)-is the most common source of BMs. MicroRNAs (miRNAs) play a crucial role in regulating gene expression, thereby contributing to tumor progression and metastatic spread. Identifying these regulatory molecules may enable a deeper understanding of the mechanisms driving LUAD brain metastasis (LUAD-BM) development and reveal therapeutic targets to prevent or limit disease progression. Methods: Next-generation RNA sequencing (RNA-seq) was performed on six LUAD-BM and six non-tumorous human brain tissue samples to assess miRNA expression profiles. Additionally, RNA-seq data from 20 primary LUAD and 15 normal lung tissue samples were obtained from The Cancer Genome Atlas (TCGA) database. MiRNAs showing the most pronounced alterations in LUAD-BM samples were selected for validation by real time quantitative polymerase chain reaction (RT-qPCR). Results: Analysis of RNA-seq data identified 229 differentially expressed (DE) miRNAs between LUAD-BM and control samples. Functional annotation analysis indicated that these DE miRNAs are key regulators of tumorigenesis and metastasis. Using the Mann-Whitney U test, ten miRNAs were confirmed to differ significantly between LUAD-BM and normal brain tissue. Receiver operating characteristic (ROC) curve analysis demonstrated their diagnostic potential. Among the ten validated miRNAs, miR-200c-3p, miR-146b-5p, and miR-3934-5p showed distinct expression patterns between primary LUAD and LUAD-BM, while miR-10a-5p, miR-210-3p, and miR-130b-3p exhibited stepwise dysregulation along the normal lung-LUAD-LUAD-BM axis, suggesting their involvement in metastatic progression. Conclusion: We identified ten miRNAs that showed preliminary ability to differentiate LUAD-BM from normal brain tissue. These findings indicate possible diagnostic and therapeutic implications. Among these, six miRNAs showed significant expression changes along the normal control-primary LUAD-LUAD-BM axis, highlighting their potential as biomarkers and therapeutic targets in BM development.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk biomarker panel brain tissue invasion lung adenocarcinoma brain metastasis miRNAs next-generation sequencing
Megjelenés:	Frontiers in Genetics. - 17 (2026), p. 1-17. -
További szerzők:	Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Géczi Dóra (1989-) (biotechnológus) Gáspár Tekla Geszti Gréta Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM128116
035-os BibID:	(scopus)85218910548 (wos)001429631100001
Első szerző:	Torner Bernadett (molekuláris biológus)
Cím:	Construction of a miRNA Panel for Differentiating Lung Adenocarcinoma Brain Metastases and Glioblastoma / Torner Bernadett, Géczi Dóra, Klekner Álmos, Balogh István, Penyige András, Birkó Zsuzsanna
Dátum:	2025
ISSN:	2072-6694
Megjegyzések:	Abstract: Background/Objectives: Brain metastases (BM) are the most common type of intracra-nial malignant tumor and are associated with high mortality. More than 50% of BM cases origi-nate from lung cancer, and lung adenocarcinoma (LUAD) is most commonly associated with the development of BM (25%). The differential diagnosis of solitary BM and glioblastoma (GBM) ? one of the most aggressive and fatal malignant brain tumors ? remains a considerable challenge. Given the major role of microRNAs (miRNAs) in regulating gene expression, their clinical po-tential as biomarkers for tumor diagnosis and prognosis offers significant promise. Methods: Next Generation RNA Sequencing (RNA-seq) was used to assess the miRNA expression profiles of 6 LUAD-BM, 6 GBM, and 6 control (non-tumoral brain tissue samples) human brain tissue samples. miRNAs exhibiting the most significant differential expression in LUAD-BM patients in comparison to both control subjects and GBM patients were selected for validation through RT-qPCR. Results: The analysis of RNA-seq data revealed the presence of 229 differentially ex-pressed miRNAs in the comparison between LUAD-BM and control samples and 46 in the com-parison between LU-AD-BM and GBM samples. Eight miRNAs were selected for further analysis, four of which were upregulated and four downregulated, based on the significant differences in their expression levels observed between the LUAD-BM samples and the other two groups, as confirmed with the Mann-Whitney U test. A functional enrichment analysis was also conducted based on a miRNA-centered target analysis performed using the miRNet tool. To assess the di-agnostic potential of these differentially expressed miRNAs, we performed a receiver operating characteristic (ROC) curve analysis. Conclusions: A panel of eight miRNAs was identified in human brain tissue samples, exhibiting high accuracy in distinguishing LUAD-BM from both GBM and control samples.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk lung adenocarcinoma brain metastasis, glioblastoma miRNAs Next-Generation Sequencing brain tissue biomarker panel
Megjelenés:	Cancers. - 17 : 4 (2025), p. 1-23. -
További szerzők:	Géczi Dóra (1989-) (biotechnológus) Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:	2017-1.2.1-NKP-2017-00002 Egyéb Bridging Fund of the Faculty of Medicine, University of Debrecen (IB) Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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