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001-es BibID:	BIBFORM135084
035-os BibID:	(scopus)105023061932 (wos)001623972300001
Első szerző:	Demeter Sarolta (orvos)
Cím:	Flow Cytometric Challenges in Plasmacytoid Dendritic Cell (pDC) Identification : limitation of BDCA-4 (CD304)-Based Gating / Demeter Sarolta, Fekete Tünde, Scholtz Beáta, Veréb Zoltán, Kemény Lajos, Bácsi Attila, Pázmándi Kitti
Dátum:	2025
ISSN:	1661-6596 1422-0067
Megjegyzések:	Plasmacytoid dendritic cells (pDCs) are a unique subset of dendritic cells specialized in rapid and robust type I interferon (IFN) production, playing critical roles in the pathogenesis and pathomechanisms of many human diseases. Accurate identification of pDCs in peripheral blood mononuclear cells (PBMCs) is challenging due to dynamic and non-exclusive specific expression of surface markers such as blood dendritic cell antigen (BDCA)-2 and BDCA-4. Although BDCA-4 is generally more stably expressed than BDCA-2, prolonged stimulation or inflammatory conditions can induce its expression on multiple non-pDC cell types, reducing the accuracy of pDC identification. Here, we thoroughly investigated BDCA-4 expression dynamics on pDCs and other PBMC subsets following prolonged activation with Toll-like receptor (TLR) 7 and TLR9 agonists. Our flow cytometry analysis revealed a significant increase in BDCA-4-positive non-pDC populations after extended stimulation, primarily corresponding to CD14+ monocytes. To overcome this limitation, we performed a gating strategy combining BDCA-4 positivity with a cocktail of non-pDC markers, enabling the exclusion of non-pDCs and accurate identification of pDCs. This approach enables the reliable identification of pDCs within heterogeneous cell populations using only two fluorescent channels in healthy conditions and even during strong activation or pathological states characterized by chronic inflammation.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk plasmacytoid dendritic cell monocyte BDCA-4 gating strategy flow cytometry
Megjelenés:	International Journal Of Molecular Sciences. - 26 : 22 (2025), p. 1-19. -
További szerzők:	Fekete Tünde (1984-) (immunológus, molekuláris biológus, mikrobiológus) Scholtz Beáta (1967-) (biokémikus, molekuláris biológus) Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus) Kemény Lajos V. (bőrgyógyász Szeged) Bácsi Attila (1967-) (immunológus) Pázmándi Kitti Linda (1984-) (molekuláris biológus, immunológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM112979
035-os BibID:	(WoS)001028670900001 (Scopus)85165107467
Első szerző:	Jambrovics Károly (biológus, gyógyszer-biotechnológus)
Cím:	ATO Increases ROS Production and Apoptosis of Cells by Enhancing Calpain-Mediated Degradation of the Cancer Survival Protein TG2 / Károly Jambrovics, Szilárd Póliska, Beáta Scholtz, Iván P. Uray, Zoltán Balajthy
Dátum:	2023
ISSN:	1422-0067
Megjegyzések:	Transglutaminase 2 (TG2) is a critical cancer cell survival factor that activates several signalling pathways to foster drug resistance, cancer stem cell survival, metastasis, inflammation, epithelial-mesenchymal transition, and angiogenesis. All-trans retinoic acid (ATRA) and chemotherapy have been the standard treatments for acute promyelocytic leukaemia (APL), but clinical studies have shown that arsenic trioxide (ATO), alone or in combination with ATRA, can improve outcomes. ATO exerts cytotoxic effects in a variety of ways by inducing oxidative stress, genotoxicity, altered signal transduction, and/or epigenetic modification. In the present study, we showed that ATO increased ROS production and apoptosis ratios in ATRA-differentiated NB4 leukaemia cells, and that these responses were enhanced when TG2 was deleted. The combined ATRA + ATO treatment also increased the amount of nuclear factor erythroid 2-related factor 2 (NRF2) transcription factor, an adaptive regulator of the cellular oxidative stress response, and calpain proteolytic activity, resulting in TG2 degradation and the reduced survival of WT leukaemia cells. We further showed that the induced TG2 protein expression was degraded in the MCF-7 epithelial cell line and primary peripheral blood mononuclear cells upon ATO treatment, thereby sensitising these cell types to apoptotic signals.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	International Journal Of Molecular Sciences. - 24 : 13 (2023), p. 1-11. -
További szerzők:	Póliska Szilárd (1978-) (biológus) Scholtz Beáta (1967-) (biokémikus, molekuláris biológus) Uray Iván Péter (1970-) (kutatóorvos) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus)
Pályázati támogatás:	129139 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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