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001-es BibID:	BIBFORM035553
Első szerző:	Bacsó Zsolt (biofizikus)
Cím:	Selected LSC applications / Zsolt Bacsó, Attila Megyeri, James F. Eliason
Dátum:	2007
ISSN:	1552-4922
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt egyetemen (Magyarországon) készült közlemény
Megjelenés:	Cytometry Part A. - 71A : 1 (2007), p. 47. -
További szerzők:	Megyeri Attila (1968-) (orvos) Eliason, James F.
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001-es BibID:	BIBFORM077446
Első szerző:	Eliason, James F.
Cím:	Potential for predicting toxicity and response of fluoropyrimidines in patients / Eliason J. F., Megyeri A.
Dátum:	2004
ISSN:	1389-4501
Megjegyzések:	The efficacy of cancer therapy is compromised by the fact that there are currently no good ways to predict which patients will benefit from treatment. This long standing goal is closer to becoming a reality as more is learned about the molecules that affect the activities of various therapeutic agents. The fluoropyrimidine antimetabolites drugs have been in clinical use for over 4 decades and the cellular proteins important for their activities have been studied in detail. The most important are the major target enzyme, thymidylate synthase (TS) and the rate limiting enzyme in the degradation pathway, dihydropyrimidine dehydrogenase (DPD), equally important for the analogue capecitabine is thymidine phosphorylase (TP), which is rate limiting for activation of this prodrug. A number of assays are available for these enzymes, including enzyme activity measurements, quantitative PCR for RNA expression and immunological methods for protein expression. With each of these methods, more clinical studies are required to validate their clinical usefulness.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban 5-fluorouracil capecitabine quantitative immunofluorescence pcr elisa thymidine phosphorylase dihydropyridine dehydrogenase
Megjelenés:	Current Drug Targets. - 5 : 4 (2004), p. 383-388. -
További szerzők:	Megyeri Attila (1968-) (orvos)
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001-es BibID:	BIBFORM077447
Első szerző:	Megyeri Attila (orvos)
Cím:	Laser Scanning Cytometry for selection of green fluorescent protein transgenic mice using small number of blood cells / Attila Megyeri, Joseph Kaplan, James F. Eliason
Dátum:	2004
ISSN:	0165-022X
Megjegyzések:	A Laser Scanning Cytometry-based method was developed for identification of transgenic mice expressing green fluorescent protein (GFP) using minute amounts of peripheral blood. The difference between the autofluorescence of cells not expressing GFP and the fluorescence of GFP expressing cells after excitation with Ar-ion laser (wavelength 488 nm) and detection of emitted fluorescent light in the green channel was high enough for unambiguous identification of the GFP expressing mice. The sensitivity of this method was estimated 1:104 for detection of rare GFP expressing cells under the conditions used. This sensitivity should be sufficient for many studies on microchimerism. Because of the possibility for relocation of the cells, this method will be particularly useful for characterizing the cells with high GFP expression using other markers of cell phenotype or conventional morphological analysis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Laser Scanning Cytometry Green fluorescent protein Microchimerism
Megjelenés:	Journal of Biochemical and Biophysical Methods. - 61 : 1-2 (2004), p. 183-187. -
További szerzők:	Kaplan, Joseph B. Eliason, James F.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM004876
035-os BibID:	WOS:000228153700002
Első szerző:	Megyeri Attila (orvos)
Cím:	Development of a stereological method to measure levels of fluoropyrimidine metabolizing enzymes in tumor sections using laser scanning cytometry / Attila Megyeri, Zsolt Bacsó, Anthony Shields, James F. Eliason
Dátum:	2005
ISSN:	1552-4922
Megjegyzések:	The enzymes thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) influence the activities of fluoropyrimidine anticancer drugs. The sensitivity of cancer cells to capecitabine, which is an oral, tumor-selective pre-prodrug of 5-fluorouracil may correlate better to the TP/DPD ratio than to levels of either enzyme alone. Our goal was to develop a quantitative immunofluorescent method for estimating the levels of TP, DPD, and their ratio in archival tumor sections. METHODS: Mouse anti-TP and rat anti-DPD monoclonal antibodies were used for parallel indirect immunofluorescent staining. The fluorescence was measured using a laser scanning cytometer (LSC; CompuCyte, Cambridge, MA) in single cells and in sections prepared from cell lines and a human tumor. The phantom contouring feature of the LSC provided a stereologic approach for collecting the fluorescence intensity data from sections. RESULTS: The relative fluorescence intensities measured in single cells or in sections of the cell lines, using single or double labeling, were similar, supporting the suitability of phantom contouring and two-color staining. Sections of the T-24 and ZR-75-1 cell lines placed on the same slide as the tumor section were used as internal standards for fluorescence measurements. The TP/DPD ratios measured in three cell lines correlated well with the cytotoxicity of 5'-deoxy-5-fluorouridine measured in vitro, indicating that the measurements are related to the biological activity of the drug. CONCLUSIONS: Plotting the data as contour maps of the topologic distribution of fluorescence intensities in tumor sections allows subsequent histopathologic examination, which may reveal features of the tumors leading to high or low ratios of these enzymes. In addition, this method can be used for any drug target/metabolic system where the key components are known and suitable antibodies are available
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Antibodies Antibodies,Monoclonal Antimetabolites,Antineoplastic Cell Line Cell Line,Tumor Cell Proliferation Cells chemistry Dihydrouracil Dehydrogenase (NADP) drug effects Dyes Enzymes enzymology Esophageal Neoplasms Floxuridine Fluorescence Fluorescent Antibody Technique Fluorescent Dyes Human Humans In Vitro Laser Scanning Cytometry metabolism methods Neoplasms pathology pharmacology Pyrimidines Research Support Thymidine Thymidine Phosphorylase egyetemen (Magyarországon) készült közlemény
Megjelenés:	Cytometry. Part A. - 64 : 2 (2005), p. 62-71. -
További szerzők:	Bacsó Zsolt (1963-) (biofizikus) Shields, Anthony Eliason, James F.
Internet cím:	elektronikus változat DOI
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