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001-es BibID:	BIBFORM044305
Első szerző:	Korponay-Szabó Ilma (gyermekgyógyász)
Cím:	Tissue Transglutaminase Is the Target in Both Rodent and Primate Tissues for Celiac Disease - Specific Autoantibodies / Korponay-Szabo Ilma R., Sulkanen Satu, Halttunen Tuula, Maurano Francesco, Rossi Mauro, Mazzarella Giuseppe, Laurila Kaija, Troncone Riccardo, Maki Markku
Dátum:	2000
ISSN:	0277-2116
Megjegyzések:	BACKGROUND:Endomysial antibodies have recently been shown to react with tissue transglutaminase. This study was undertaken to investigate whether the tissue distribution of transglutaminase is also compatible with reticulin, jejunal, and fibroblast autoantibody binding patterns.METHODS:Sera from patients with and without celiac disease, monoclonal tissue transglutaminase antibodies, and sera from mice parenterally immunized against commercially available tissue transglutaminase, transglutaminase complexed with gliadin, or gliadin were used in indirect immunofluorescence and double-staining studies using both rodent and primate tissues as substrates. Also, antibody competition, affinity chromatography, and potassium thiocyanate extraction studies were undertaken.RESULTS:Tissue transglutaminase antibody binding patterns were identical with the extracellular binding patterns seen with celiac patient sera. Human umbilical cord-derived fibroblasts exhibited both cytoplasmic and extracellular matrix staining. Double staining with patients' sera and tissue transglutaminase antibodies showed complete overlapping. Tissue transglutaminase effectively absorbed reticulin-endomysial antibodies from celiac sera, and patients' sera blocked the staining of the monoclonal tissue transglutaminase antibodies. Potassium thiocyanate extraction abolished the staining patterns, but they were elicited again after readdition of tissue transglutaminase.CONCLUSIONS:Reticulin, endomysial, and jejunal antibodies detect transglutaminase in both rodent and primate tissues, indicating that these tissue autoantibodies are identical
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Pediatric Gastroenterology And Nutrition. - 31 : 5 (2000), p. 520-527. -
További szerzők:	Sulkanen, Satu Halttunen, Tuula Maurano, Francesco Rossi, Mauro Mazzarella, Giuseppe Laurila, Kaija Troncone, Riccardo Mäki, Markku
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM044307
Első szerző:	Sulkanen, Satu
Cím:	Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease / Sulkanen Satu, Halttunen Tuula, Laurila Kaija, Kolho Kaija-Leena, Korponay-Szabó Ilma R., Sarnesto Annikki, Savilahti Erkki, Collin Pekka, Mäki Markku
Dátum:	1998
ISSN:	0016-5085
Megjegyzések:	BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS:Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and gliadin antibodies with ELISA. RESULTS:The calcium-activated tissue transglutaminase autoantibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoantibody ELISA test correlated well with the endomysial antibody test. Tissue transglutaminase autoantibody ELISA showed a clearly better predictive potential than the IgA class gliadin antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS:Calcium-activated tissue transglutaminase autoantibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Gastroenterology. - 115 : 6 (1998), p. 1322-1328. -
További szerzők:	Halttunen, Tuula Laurila, Kaija Kolho, Kaija-Leena Korponay-Szabó Ilma (1959-) (gyermekgyógyász) Sarnesto, Annikki Savilahti, Erkki Collin, Pekka Mäki, Markku
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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