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001-es BibID:	BIBFORM120894
035-os BibID:	(Scopus)85191684008
Első szerző:	Mujitaba, Malam Abulbashar
Cím:	The Influence of Three Commercial Soy Lecithin-Based Semen Extenders and Two Spermatozoa Concentrations on the Quality of Pre-Freeze and Post-Thaw Ram Epididymal Spermatozoa / Malam Abulbashar Mujitaba, Gabriella Kútvölgyi, Judit Radnai Szentpáli, Viktória Johanna Debnár, Alexandra Tokár, Nóra Vass, Szilárd Bodó
Dátum:	2024
ISSN:	2076-2615
Megjegyzések:	There are limited studies on the factors affecting the success of ram epididymal spermatozoa (REPS) cryopreservation. On this note, the current study assessed the influence of three commercial soy lecithin-based semen extenders, AndroMed? (AND), BioXcell? (BIO), and OviXcell? (OVI), and two concentrations (400 ? 106 vs. 200 ? 106 spermatozoa/mL) on the pre-freeze and post-thaw quality of REPS. The REPS were retrieved from nine adult rams' testes and diluted with each of the three extenders to both concentrations. Straws were frozen manually. Standard motility (SMP) and kinematic parameters (KPs) were assessed via a CASA, while spermatozoa viability, morphology, and acrosomal integrity were assessed via the Kovács?Foote staining technique. The concentration did not significantly affect the pre-freeze and post-thaw SMP and KPs of REPS. BIO and OVI had significantly higher pre-freeze and post-thaw BCFs, post-thaw VAP, and the percentage of all intact heads than AND. In contrast, AND had a significantly lower percentage of REPS with tail defects than BIO and OVI. The 400 * 106 spermatozoa/mL concentration resulted in a significantly higher percentage of all intact heads than the 200 * 106 spermatozoa/mL concentration. Freezing significantly increased tail defects and decreased the percentage of REPS with distal cytoplasmic droplets. The cryopreservation of REPS at the 400 ? 106 spermatozoa/mL concentration is recommended. All three extenders must be optimized to preserve the viability, membrane integrity, and better normal morphology of REPS; the reason for increased tail abnormality after the freezing/thawing process needs to be studied.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk AndroMed BioXcell OviXcell soy lecithin ram epididymal spermatozoa cryopreservation membrane integrity
Megjelenés:	Animals. - 14 : 8 (2024), p. 1-16. -
További szerzők:	Kútvölgyi Gabriella Szentpáli-Radnai Judit Debnár Viktória Johanna Tokár Alexandra Vass Nóra (1983-) (állatorvos) Bodó Szilárd
Pályázati támogatás:	Stipendium Hungaricum Scholarship Program Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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