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1.

001-es BibID:BIBFORM105289
Első szerző:Barnáné Szabó Zsuzsanna
Cím:Comparison of different non-destructive phenotyping approaches to assess acute phytotoxic effects of arsenic treatments in duckweed tests / Zsuzsanna Barnáné Szabó, Muhammad Irfan, Anna Hepp, Ilona Mészáros, Viktor Oláh
Dátum:2021
Megjegyzések:We assessed the acute phytotoxic effects of arsenite (AsO3 3- ) and arsenate (AsO4 3- ) on laboratory cultures of giant duckweed (Spirodela polyrhiza) in 3 days-long treatments in order to compare different rapid, nondestructive phenotyping methods. The growth of cultures was jointly measured with the photochemical efficiency by means of in vivo chlorophyll fluorescence imaging, and the relative ratio of anthocyaninaccumulating frond surfaces via digital imaging, respectively. Our results revealed stronger phytotoxicity of arsenite (EC50=2,3-2,7 mg As l-1 ) compared to arsenate (EC50=16-22 mg As l-1 ) under the applied test conditions. The calculated effective concentrations indicated similar sensitivities of the growth- and chlorophyll fluorescence-based parameters. At lower arsenic concentrations, however, growth inhibition was more sensitive. The relative share of anthocyanic frond surfaces increased due to arsenate treatments but decreased in response to arsenite. Therefore, this parameter is less applicable in direct characterization of phytotoxicity but offer additional information on physiological effects.
ISBN:978-963-8221-82-7
Tárgyszavak:Természettudományok Biológiai tudományok előadáskivonat
könyvrészlet
arsenic
duckweed test
chlorophyll fluorescence
anthocyanin
Megjelenés:XVI. Kárpát-medencei Környezettudományi Konferencia = 16th Carpathian Basin Conference for Environmental Sciences: absztrakt kötet = abstract book / szerk. Cseresznyés Dóra, Király Csilla. - p. 240-245. -
További szerzők:Irfan, Muhammad (1995-) (PhD Environmental Sciences) Hepp Anna (1991-) (biotechnológus) Mészáros Ilona (1952-) (biológus) Oláh Viktor (1980-) (biológus)
Pályázati támogatás:EFOP-3.4.3-16-2016-00016
EFOP
ÚNKP-19-3-I-81
Egyéb
EFOP-3.4.3-16-2016-00012
EFOP
NKFIH-OTKA-FK-134296
Egyéb
2020-1-DE01-KA203-0057
Egyéb
GINOP-2.3.2-15-2016- 00031
GINOP
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2.

001-es BibID:BIBFORM118127
035-os BibID:(WoS)001152827800001 (Scopus)85183116796
Első szerző:Irfan, Muhammad (PhD Environmental Sciences)
Cím:Comparative Phytotoxicity of Metallic Elements on Duckweed Lemna gibba L. Using Growth- and Chlorophyll Fluorescence Induction-Based Endpoints / Muhammad Irfan, Ilona Mészáros, Sándor Szabó, Viktor Oláh
Dátum:2024
ISSN:2223-7747
Megjegyzések:In this study, we exposed a commonly used duckweed species-Lemna gibba L.-to twelve environmentally relevant metals and metalloids under laboratory conditions. The phytotoxic effects were evaluated in a multi-well-plate-based experimental setup by means of the chlorophyll fluorescence imaging method. This technique allowed the simultaneous measuring of the growth and photosynthetic parameters in the same samples. The inhibition of relative growth rates (based on frond number and area) and photochemical efficiency (F-v/F-o and Y(II)) were both calculated from the obtained chlorophyll fluorescence images. In the applied test system, growth-inhibition-based phytotoxicity endpoints proved to be more sensitive than chlorophyll-fluorescence-based ones. Frond area growth inhibition was the most responsive parameter with a median EC50 of 1.75 mg L-1, while F-v/F-o, the more responsive chlorophyll-fluorescence-based endpoint, resulted in a 5.34 mg L-1 median EC50 for the tested metals. Ag (EC50 0.005-1.27 mg L-1), Hg (EC50 0.24-4.87 mg L-1) and Cu (EC50 0.37-1.86 mg L-1) were the most toxic elements among the tested ones, while As(V) (EC50 47.15-132.18 mg L-1), Cr(III) (EC50 6.22-19.92 mg L-1), Se(VI) (EC50 1.73-10.39 mg L-1) and Zn (EC50 3.88-350.56 mg L-1) were the least toxic ones. The results highlighted that multi-well-plate-based duckweed phytotoxicity assays may reduce space, time and sample volume requirements compared to the standard duckweed growth inhibition tests. These benefits, however, come with lowered test sensitivity. Our multi-well-plate-based test setup resulted in considerably higher median EC50 (3.21 mg L-1) for frond-number-based growth inhibition than the 0.683 mg L-1 median EC50 derived from corresponding data from the literature with standardized Lemna-tests. Under strong acute phytotoxicity, frond parts with impaired photochemical functionality may become undetectable by chlorophyll fluorometers. Consequently, the plant parts that are still detectable display a virtually higher average photosynthetic performance, leading to an underestimation of phytotoxicity. Nevertheless, multi-well-plate-based duckweed phytotoxicity assays, combined with chlorophyll fluorescence imaging, offer definite advantages in the rapid screening of large sample series or multiple species/clones. As chlorophyll fluorescence images provide information both on the photochemical performance of the test plants and their morphology, a joint analysis of the two endpoint groups is recommended in multi-well-plate-based duckweed phytotoxicity assays to maximize the information gained from the tests.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
békalencse
fitotoxicitás
ökotoxikológia
növekedésgátlás
klorofill fluoreszcencia indukció
nehézfém
Megjelenés:Plants-Basel. - 13 : 2 (2024), p. 1-16. -
További szerzők:Mészáros Ilona (1952-) (biológus) Szabó Sándor (biológus) Oláh Viktor (1980-) (biológus)
Pályázati támogatás:FK 134296
OTKA
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3.

001-es BibID:BIBFORM118729
035-os BibID:(WoS)001196813800001 (Scopus)85185442437
Első szerző:Oláh Viktor (biológus)
Cím:Frond-level analyses reveal functional heterogeneity within heavy metal-treated duckweed colonies / Viktor Oláh, Kamilla Kosztankó, Muhammad Irfan, Zsuzsanna Barnáné Szabó, Marcel A. K Jansen, Sándor Szabó, Ilona Mészáros
Dátum:2024
ISSN:2667-064X
Megjegyzések:Duckweeds (Lemnaceae) rapidly produce clonal populations that make them ideal models in plant physiological and ecotoxicological research. Yet, despite their genetic homogeneity, duckweed colonies are clusters of successively produced fronds with different ontogenetic states, and this heterogeneity has rarely been studied. We analyzed frond-level photosynthetic responses of three duckweed species (Spirodela polyrhiza, Landoltia punctata and Lemna minor) to hexavalent chromium and nickel by means of chlorophyll fluorescence imaging. Our aim was to test whether fronds, or frond zones in their different developmental stages respond differently to heavy metal stress. Different response patterns were found for the three tested species. Young fronds of S. polyrhiza were more sensitive to chromate but less affected by nickel compared to mature ones, while in La. punctata the opposite was found. In Le. minor cultures, young fronds were more sensitive to both heavy metals compared to mature ones. Within-frond patterns indicated that chromate inhibited the marginal frond regions strongest. In La. punctata and Le. minor, nickel was most detrimental in the developing frond regions. In contrast, in S. polyrhiza the middle zone of both young and mature fronds was affected strongest by nickel. The observed patterns suggest that internal redistribution of toxicants plays a key role in shaping duckweed responses. Results highlight that ontogenetically different parts of duckweed plants respond differently to environmental stimuli. Since duckweed-based impact studies and economical applications rely strongly on the interactions between the duckweed colony and its environment, frond-, and within-frond level analysis of duckweed responses comprise a unique tool to reveal uptake, re-distribution and mode of action of environmentally relevant substances.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
békalencse
frond
ontogenezis
klorofill fluoreszcencia leképezés
nehézfém stressz
Megjelenés:Plant Stress. - 11 (2024), p. 1-11. -
További szerzők:Kosztankó Kamilla Irfan, Muhammad (1995-) (PhD Environmental Sciences) Barnáné Szabó Zsuzsanna (2000-) Jansen, Marcel A. K. Szabó Sándor (1956-) (zoológus) Mészáros Ilona (1952-) (biológus)
Pályázati támogatás:FK 134296
OTKA
ÚNKP-23-5
Egyéb
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4.

001-es BibID:BIBFORM107750
035-os BibID:(WOS)000909169600001 (Scopus)85145834280
Első szerző:Oláh Viktor (biológus)
Cím:Species- and Metal-Specific Responses of the Ionome of Three Duckweed Species under Chromate and Nickel Treatments / Viktor Oláh, Muhammad Irfan, Zsuzsanna Barnáné Szabó, Zsófi Sajtos, Ágota Zsófia Ragyák, Boglárka Döncző, Marcel A. K. Jansen, Sándor Szabó, Ilona Mészáros
Dátum:2023
ISSN:2223-7747
Megjegyzések:In this study, growth and ionomic responses of three duckweed species were analyzed, namely Lemna minor, Landoltia punctata, and Spirodela polyrhiza, were exposed for short-term periods to hexavalent chromium or nickel under laboratory conditions. It was found that different duckweed species had distinct ionomic patterns that can change considerably due to metal treatments. The results also show that, because of the stress-induced increase in leaf mass-to-area ratio, the studied species showed different order of metal uptake efficiency if plant area was used as unit of reference instead of the traditional dry weight-based approach. Furthermore, this study revealed that mu XRF is applicable in mapping elemental distributions in duckweed fronds. By using this method, we found that within-frond and within-colony compartmentation of metallic ions were strongly metal- and in part species-specific. Analysis of duckweed ionomics is a valuable approach in exploring factors that affect bioaccumulation of trace pollutants by these plants. Apart from remediating industrial effluents, this aspect will gain relevance in food and feed safety when duckweed biomass is produced for nutritional purposes.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Plants-Basel. - 12 : 1 (2023), p. 1-16. -
További szerzők:Irfan, Muhammad (1995-) (PhD Environmental Sciences) Barnáné Szabó Zsuzsanna (2000-) Sajtos Zsófi (1994-) (kémikus, okleveles analitikus vegyész) Dienes Ágota Zsófia (1997-) (vegyészmérnök) Döncző Boglárka (1987-) (biológiatanár-földrajztanár) Jansen, Marcel A. K. Szabó Sándor Mészáros Ilona (1952-) (biológus)
Pályázati támogatás:FK 134296
OTKA
2.3.3-15-2016-00029
GINOP
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5.

001-es BibID:BIBFORM107745
035-os BibID:(WOS)000738686000001 (Scopus)85121026642
Első szerző:Oláh Viktor (biológus)
Cím:Chlorophyll Fluorescence Imaging-Based Duckweed Phenotyping to Assess Acute Phytotoxic Effects / Viktor Oláh, Anna Hepp, Muhammad Irfan, Ilona Mészáros
Dátum:2021
ISSN:2223-7747
Megjegyzések:Duckweeds (Lemnaceae species) are extensively used models in ecotoxicology, and chlorophyll fluorescence imaging offers a sensitive and high throughput platform for phytotoxicity assays with these tiny plants. However, the vast number of potentially applicable chlorophyll fluorescence-based test endpoints makes comparison and generalization of results hard among different studies. The present study aimed to jointly measure and compare the sensitivity of various chlorophyll fluorescence parameters in Spirodela polyrhiza (giant duckweed) plants exposed to nickel, chromate (hexavalent chromium) and sodium chloride for 72 h, respectively. The photochemistry of Photosystem II in both dark- and light-adapted states of plants was assessed via in vivo chlorophyll fluorescence imaging method. Our results indicated that the studied parameters responded with very divergent sensitivity, highlighting the importance of parallelly assessing several chlorophyll fluorescence parameters. Generally, the light-adapted parameters were more sensitive than the dark-adapted ones. Thus, the former ones might be the preferred endpoints in phytotoxicity assays. Fv/Fm, i.e., the most extensively reported parameter literature-wise, proved to be the least sensitive endpoint; therefore, future studies might also consider reporting Fv/Fo, as its more responsive analogue. The tested toxicants induced different trends in the basic chlorophyll fluorescence parameters and, at least partly, in relative proportions of different quenching processes, suggesting that a basic distinction of water pollutants with different modes of action might be achievable by this method. We found definite hormetic patterns in responses to several endpoints. Hormesis occurred in the concentration ranges where the applied toxicants resulted in strong growth inhibition in longer-term exposures of the same duckweed clone in previous studies. These findings indicate that changes in the photochemical efficiency of plants do not necessarily go hand in hand with growth responses, and care should be taken when one exclusively interprets chlorophyll fluorescence-based endpoints as general proxies for phytotoxic effects.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
békalencse
klorofill fluoreszcencia indukció
ökotoxikológia
nehézfém
Megjelenés:Plants-Basel. - 10 : 12 (2021), p. 1-19. -
További szerzők:Hepp Anna (1991-) (biotechnológus) Irfan, Muhammad (1995-) (PhD Environmental Sciences) Mészáros Ilona (1952-) (biológus)
Pályázati támogatás:FK 134296
OTKA
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6.

001-es BibID:BIBFORM132352
Első szerző:Xuan, Pham Thi Hong
Cím:Lemna gibba Clones Show Differences in Phenotypic Responses to the Light Environment / Pham Thi Hong Xuan, Raja Amri, Nguyen Phuong Bach, Muhammad Irfan, Manuela Bog, Klaus J. Appenroth, K. Sowjanya Sree, Marcel A. K. Jansen, Sándor Szabó, Ilona Mészáros, Viktor Oláh
Dátum:2025
ISSN:2223-7747
Megjegyzések:Duckweeds are aquatic plants with a worldwide distribution that can thrive under very contrasting ambient conditions due to their diversity and high phenotypic plasticity. In this study, we analyzed and compared the responses of four clones (i.e., distinct accessions) of Lemna gibba to two different light intensities. The clones represented different geographical origins and, in addition to two diploid cytotypes, included one tetraploid mutant and a triploid interspecific hybrid. We hypothesized that clonal origin had an effect on light acclimation. We studied growth, morphological (frond size and shape, mass-to-area ratio), and photosynthetic (pigment composition, chlorophyll fluorescence induction) traits of these clones to test whether light acclimation was a conserved process or whether clonespecific strategies could be found. We also analyzed frond-level photosynthetic adjustment during ontogenesis to track how light acclimation evolved in developing fronds. Our results confirmed that even clones of the same duckweed species and a hybrid of closely related species followed partially different strategies in acclimation to ambient conditions. This acclimation involved various morphological, physiological, and biochemical adjustments but happened in a very short time window at the early life stage when young, still-developing fronds could flexibly achieve an optimized phenotype. In addition to explaining the worldwide success of duckweeds in colonizing very contrasting habitats, our results also highlight the importance of approaching duckweed responses at the frond level, where the actual acclimation takes place.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
duckweed
frond
light acclimation
biodiversity
photosynthesis
photosynthetic pigments
chlorophyll fluorescence
Megjelenés:Plants-Basel. - 14 : 18 (2025), p. 1-22. -
További szerzők:Amri, Raja Bach, Nguyen Phuong Irfan, Muhammad (1995-) (PhD Environmental Sciences) Bog, Manuela Appenroth, Klaus-Juergen Sree, K. Sowjanya Jansen, Marcel A. K. Szabó Sándor (1956-) (zoológus) Mészáros Ilona (1952-) (biológus) Oláh Viktor (1980-) (biológus)
Pályázati támogatás:134296
OTKA
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