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001-es BibID:	BIBFORM073726
Első szerző:	Andrássy László
Cím:	Laser-induced plasma spectroscopy (LIPS) : use of a geological tool in assessing bone mineral content / Andrássy László, Gomez Izabella, Horváth Ágnes, Gulyás Katalin, Pethö Zsófia, Juhász Balázs, Bhattoa Harjit Pal, Szekanecz Zoltan
Dátum:	2018
ISSN:	0268-8921
Megjegyzések:	Bone may be similar to geological formulations in many ways. Therefore, it may be logical to apply laser-based geological techniques in bone research. The mineral and element oxide composition of bioapatite can be estimated by mathematical models. Laser-induced plasma spectrometry (LIPS) has long been used in geology. This method may provide a possibility to determine the composition and concentration of element oxides forming the inorganic part of bones. In this study, we wished to standardize the LIPS technique and use mathematical calculations and models in order to determine CaO distribution and bone homogeneity using bovine shin bone samples. We used polished slices of five bovine shin bones. A portable LIPS instrument using high-power Nd++YAG laser pulses has been developed (OpLab, Budapest). Analysis of CaO distribution was carried out in a 10 ? 10 sampling matrix applying 300-?m sampling intervals. We assessed both cortical and trabecular bone areas. Regions of interest (ROI) were determined under microscope. CaO peaks were identified in the 200?500 nm wavelength range. A mathematical formula was used to calculate the element oxide composition (wt%) of inorganic bone. We also applied two accepted mathematical approaches, the Bartlett's test and frequency distribution curve-based analysis, to determine the homogeneity of CaO distribution in bones. We were able to standardize the LIPS technique for bone research. CaO concentrations in the cortical and trabecular regions of B1?5 bones were 33.11 ? 3.99% (range 24.02?40.43%) and 27.60 ? 7.44% (range 3.58?39.51%), respectively. CaO concentrations highly corresponded to those routinely determined by ICP-OES. We were able to graphically demonstrate CaO distribution in both 2D and 3D. We also determined possible interrelations between laser-induced craters and bone structure units, which may reflect the bone structure and may influence the heterogeneity of CaO distributions. By using two different statistical methods, we could confirm if bone samples were homogeneous or not with respect to CaO concentration distribution. LIPS, a technique previously used in geology, may be included in bone research. Assessment of element oxide concentrations in the inorganic part of bone, as well as mathematical calculations may be useful to determine the content of CaO and other element oxides in bone, further analyze bone structure and homogeneity and possibly apply this research to normal, as well as diseased bones.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk laser-induced plasma spectrometry LIPS Element oxide CaO Mathematical model Bioapatite Bartlett's test frequency characteristic homogeneity
Megjelenés:	Lasers In Medical Science. - 33 : 6 (2018), p. 1225-1236. -
További szerzők:	Gomez Izabella (1981-) (orvos) Horváth Ágnes (1985-) (reumatológus) Gulyás Katalin (reumatológus) Pethő Zsófia (1981-) (reumatológus, immunológus) Juhász Balázs (1973-) (orvos, onkológus) Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Szekanecz Zoltán (1964-) (reumatológus, belgyógyász, immunológus)
Pályázati támogatás:	105073 OTKA 4.2.4.A/2-11/1-2012-0001 TÁMOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM124648
035-os BibID:	(Scopus)85206482558 (WoS)001334123400001
Első szerző:	Szekanecz Zoltán (reumatológus, belgyógyász, immunológus)
Cím:	Bones or Stones : how Can We Apply Geophysical Techniques in Bone Research? / Zoltán Szekanecz, Anikó Besnyi, Péter Kónya, Judit Füri, Edit Király, Éva Bertalan, György Falus, Beatrix Udvardi, Viktória Kovács-Kis, László Andrássy, Gyula Maros, Tamás Fancsik, Zsófia Pethő, Izabella Gomez, Ágnes Horváth, Katalin Gulyás, Balázs Juhász, Katalin Hodosi, Zsuzsanna Sándor, Harjit P. Bhattoa, István J. Kovács
Dátum:	2024
ISSN:	1661-6596 1422-0067
Megjegyzések:	Some studies have used physical techniques for the assessment of bone structure and composition. However, very few studies applied multiple techniques, such as those described below, at the same time. The aim of our study was to determine the chemical and mineralogical/organic composition of bovine tibial bone samples using geophysical/geochemical reference techniques. X-ray diffraction (XRD), thermogravimetry (TG), Fourier-transform infrared spectrometry with at tenuated total reflectance accessory (FTIR-ATR), inductively coupled mass spectrometry (ICP-MS) and inductively coupled optical emission spectrometry (ICP-OES) were applied to measure the organic and inorganic composition of 14 bovine bone samples. In addition, peripheral quantitative CT (QCT) was used to assess BMD in these bones. We were able to define the total composition of the studied bone samples. ICP-OES and ICP-MS techniques were used to determine the major and trace element composition. The X-ray analysis could detect inorganic crystalline compounds of bones, such as bioapatite, and its degree of ordering, indicating whether the bones belong to a younger or older individual. The total volatile content of the samples was calculated using TG and resulted in about 35 weight% (wt%). This, together with the 65 wt% total resulting from the chemical analysis (i.e., inorganic components), yielded a total approaching 100 wt%. As a large portion of the volatile content (H2O, CO2 , etc.) was liberated from the organic components and, subordinately, from bioap atite, it could be concluded that the volatile-to-solid ratio of the examined bone samples was ~35:65. The FTIR-ATR analysis revealed that the organic portion consists of collagens containing amide groups, as their typical bands (OH, CH, CO, NC) were clearly identified in the infrared spectra. Numerous parameters of bone composition correlated with BMD as determined by QCT. In conclu sion, we performed a complex evaluation of bovine bones to test multiple geophysical/geochemical techniques in bone research in association with QCT bone densitometry. From a medical point of view, the composition of the studied bones could be reliably examined by these methods.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk bone X-ray diffraction thermogravimetry Fourier-transform infrared spectrometry with attenuated total reflectance accessory inductively coupled mass spectrometry inductively coupled optical emission spectrometry
Megjelenés:	International Journal Of Molecular Sciences. - 25 : 19 (2024), p. 1-16. -
További szerzők:	Besnyi Anikó Kónya Péter Füri Judit Király Edit Bertalan Éva Falus György (Budapest) Udvardi Beatrix (Budapest) Kovács-Kis Viktória Andrássy László Maros Gyula Fancsik Tamás Pethő Zsófia (1981-) (reumatológus, immunológus) Gomez Izabella (1981-) (orvos) Horváth Ágnes (1985-) (reumatológus) Gulyás Katalin (reumatológus) Juhász Balázs (1973-) (orvos, onkológus) Hódosi Katalin Jakabné Sándor Zsuzsanna Bhattoa Harjit Pal (1973-) (laboratóriumi szakorvos) Kovács István János
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