Találati lista | Tudóstér

001-es BibID:	BIBFORM078531
035-os BibID:	(WoS)000472509400011 (Scopus)85065183537
Első szerző:	Dobránszki Judit (biológus)
Cím:	mRNA transcription profile of potato (Solanum tuberosum L.) exposed to ultrasound during different stages of in vitro plantlet development / Judit Dobránszki, Norbert Hidvégi, Andrea Gulyás, Jaime A. Teixeira da Silva
Dátum:	2019
ISSN:	0167-4412 1573-5028
Megjegyzések:	Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlying the physiological response of potato to US, single-node segments of four-week-old in vitro plantlets were subjected to US at 35 kHz for 20 min. Following mRNA purification, 10 cDNA libraries were assessed by RNA-seq. Significantly differentially expressed genes (DEGs) were categorized by gene ontology or Kyoto Encyclopedia of Genes and Genomes identifiers. The expression intensity of 40,430 genes was studied. Several hundred DEGs associated with biosynthesis, carbohydrate metabolism and catabolism, cellular protein modification, and response to stress, and which were expressed mainly in the extracellular region, nucleus, and plasma membrane, were either up- or down-regulated in response to US. RT-qPCR was used to validate RNA-seq data of 10 highly up- or down-regulated DEGs, and both Spearman and Pearson correlations between SeqMonk LFC and RT-qPCR LFC were highly positive (0.97). This study examines how some processes evolved over time (0 h, 24 h, 48 h, 1 week and 4 weeks) after an abiotic stress (US) was imposed on in vitro potato explants, and provides clues to the temporal dynamics in DEG-based enzyme functions in response to this stress. Despite this abiotic stress, plantlets survived.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Abiotic stress Antioxidant DEG Enzyme Plant growth Ultrasonication Wounding
Megjelenés:	Plant Molecular Biology. - 100 : 4-5 (2019), p. 511-525. -
További szerzők:	Hidvégi Norbert (1986-) Gulyás Andrea (1985-) (Növényi molekuláris genetika és biotechnológia) Teixeira da Silva, Jaime A.
Pályázati támogatás:	20428-3/2018/FEKUTSTRAT FIKP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM135604
Első szerző:	Ivanizs László
Cím:	Introgression of barley chromosome arms 4H and 6H into wheat via Robertsonian translocations: GBS-assisted structural analysis and impact on grain nutrient composition / László Ivanizs, Eszter Gaál, Klaudia Kruppa, András Farkas, Péter Mikó, Edina Türkösi, Marianna Rakszegi, Péter Kovács, Balázs Kalapos, Andrea Gulyás, Norbert Hidvégi, Kitti Szőke-Pázsi, Márta Molnár-Láng, Éva Szakács, Mahmoud Said, Jan Bartoš, Tünde Pusztahelyi, Dimitar Douchkov, István Molnár
Dátum:	2026
ISSN:	0167-4412
Megjegyzések:	In addition to wild relatives, cultivated Triticeae species such as barley can also serve as an excellent gene source to improve the agronomic traits of bread wheat. The Asakaze-Manas addition lines previously developed in Martonvásár exhibit low fertility and unstable inheritance; nonetheless, they could serve as 'bridge materials' for the incorporation of barley chromatin into the wheat genome. By leveraging the centric breakage-fusion mechanism of unpaired chromosomes and the gametocidal effect of chromosome 2C from Aegilops cylindrica, the 4H and 6H addition lines were used to develop genetically stable translocation genotypes. In situ hybridization and molecular marker analyses were applied to identify the wheat and barley chromosome arms in the compensatory translocations, designated as T6HS.6BL, T6BS.6HL and T4BS.4HL. These results were confirmed by GBS read coverage analysis, which revealed a putative pericentric inver- sion in the barley chromosome arms involved in the T6HS.6BL and T6BS.6HL. The comparable agronomic performance of the centric fusion lines indicates that these chromosomal rearrangements had minimal, if any, negative impact relative to the parental wheat varieties. The newly developed translocation lines had an increased amount of minerals such as iron, suggesting that they could serve as promising genetic material for the biofortification of hexaploid wheat.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Wheat-barley translocation In situ hybridization Genotyping-by-sequencing Read coverage analysis Chromosome-mediated gene transfer Mineral components
Megjelenés:	Plant Molecular Biology. - 116 : 2 (2026), p. 1-23. -
További szerzők:	Gaál Eszter Kruppa Klaudia Farkas András Mikó Péter Türkösi Edina Rakszegi Marianna Kovács Péter Kalapos Balázs Gulyás Andrea (1985-) (Növényi molekuláris genetika és biotechnológia) Hidvégi Norbert (1986-) Szőke-Pázsi Kitti Molnár-Láng Márta Szakács Éva Said, Mahmoud Bartos, Jan Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító) Douchkov Dimitar Molnár István
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM085313
Első szerző:	Teixeira da Silva, Jaime A.
Cím:	Transcriptomic Response of In Vitro Potato (Solanum tuberosum L.) to Piezoelectric Ultrasound / Jaime A. Teixeira da Silva, Norbert Hidvégi, Andrea Gulyás, Bianka Tóth, Judit Dobránszki
Dátum:	2020
ISSN:	0735-9640
Megjegyzések:	As part of a wider project to assess the impact of ultrasound on in vitro plant growth, this paper aimed to determine whether the application of piezoelectric ultrasound (PE-US) would induce changes to the transcriptome of in vitro potato (Solanum tuberosum L.). After exposing explants (single-node segments with a single leaf) to PE-US (35 kHz; 70 W) for 20 min, the effect of this stressor was determined at 0 h, 24 h, 48 h, 1 week, and 4 weeks to assess the possible immediate and residual effects of PE-US on the potato transcriptome. After mRNA was isolated, bioinformatic processing and analysis of RNA-seq datasets, KEGG analysis revealed that 24% of up-regulated biological processes were a response to stress immediately after the application of PE-US (reducing to 11% and 9% at 48 h and 4 weeks, respectively). After assessing a total of 40,430 genes for expression intensity at these five time intervals, it was found that 138, 72, 18, 5, and 59 differentially expressed genes (DEGs), respectively, were significantly up-regulated, while 6, 82, 96, 172, and 107 DEGs, respectively, were down-regulated. DEGs coding for universal stress protein, chitinase, catalase, zinc finger proteins, 21 transcription factors, glutathione S-transferase, and 17 heat shock proteins, mainly Hsp70, Hsp20, and Hsp90 proteins, and possibly the first of such discovery in US-based plant stress research, was detected. Following the validation of RNA-seq data by RT-qPCR, between SeqMonk LFC and RT-qPCR LFC, the Spearman and Pearson correlation coefficients were 0.86 and 0.90, respectively. Plantlets exposed to PE-US had significantly shorter shoots but significantly longer roots as well as higher shoot and root fresh weight, while chl b and total chl were significantly lower but the chl a/b ratio was significantly higher in plantlets exposed to PE-US. PE-US thus constituted an acute abiotic stress, but by 4 weeks, in vitro potato plantlets managed to mitigate the stress through the production of antioxidant enzyme systems and other functional metabolic changes.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Abiotic stress Chlorophyll Differentially expressed genes Enzymes Heat shock proteins Plant growth RT-qPCR Wounding
Megjelenés:	Plant Molecular Biology Reporter. - 38 : 3 (2020), p. 404-418. -
További szerzők:	Hidvégi Norbert (1986-) Gulyás Andrea (1985-) (Növényi molekuláris genetika és biotechnológia) Tóth Bianka (1988-) (biológus) Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:	NKFIH-1150-6/2019 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: