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001-es BibID:	BIBFORM099048
Első szerző:	Dzsudzsák Erika
Cím:	Profiling of lactate dehydrogenase isoenzymes in COVID-19 disease / Erika Dzsudzsák, Renáta Sütő, Marianna Pócsi, Miklós Fagyas, Zoltán Szentkereszty, Béla Nagy Jr.
Dátum:	2021
Megjegyzések:	Introduction Serum total lactate dehydrogenase (LDH) activity was elevated and showed a positive correlation with disease severity and outcome in severe COVID-19 disease. However, it is still unknown whether the relative abundance or calculated activity of any LDH isoenzyme is predominately increased in COVID-19 subjects. Methods Twenty-two consecutive patients suffered from moderate or severe COVID-19 pneumonia were recruited into this study who showed enhanced total LDH activity. The ratio of LDH isoenzyme activities was further investigated using gel electrophoresis (Hydragel, Sebia) with densitometric evaluation. Calculated act ivity values of these isoenzymes were correlated with routine laboratory parameters, the degree of lungparenchymal affection based on chest CT and clinical outcome. Results Total LDH activity was raised in the range of 272-2141 U/L and significantly correlated with calculated LDH-3 and LDH-4 activities (r=0.765, P=0.0001; and r=0.783, P=0.0001, respectively). In contrast, the relative abundance of neither LDH isoenzyme was exclusively abnormal in COVID-19 patients. Calculated activity of LDH-3 and LDH-4 demonstrated a modest but statistically significant association with serum ferritin (r=0.437, P=0.042; r=0.505, P=0.016, respectively). When the relationship between the severity of pulmonary affection by SARS-CoV-2 infection and relative abundance of LDH isoenzymes was studied, a larger ratio of mid-zone fractions was observed in the presence of ? 50% lung parenchymal involvement. Finally, regardless of LDH isoenzyme pattern, abnormal relat ive ratio of LDH-4 and higher calculated LDH-3 and LDH-4 activity values were detected in subjects with unfavorable outcome. Conclusion No characteristic profile of LDH isoenzymes can be detected in COVID-19 pneumonia, however, elevated activities of LDH-3 and LDH-4 are associated with worse clinical outcomes.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk SARS-Cov-2 COVID-19 inflammation LDH electrophoresis clinical outcome
Megjelenés:	The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine. - 32 : 4 (2021), p. 432-441. -
További szerzők:	Sütő Renáta (1986-) (aneszteziológus) Pócsi Marianna (1989-) (klinikai laboratóriumi kutató) Fagyas Miklós (1984-) (orvos) Szentkereszty Zoltán Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos)
Pályázati támogatás:	FK 135327 OTKA FK 128809 OTKA ÚNKP21-3-I-DE-255 Egyéb ÚNKP-21-5-DE-458 Egyéb BO/00069/21/5 MTA
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001-es BibID:	BIBFORM116479
035-os BibID:	(scopus)85176548280 (WoS)001099491300001
Első szerző:	Illési Ádám (orvos)
Cím:	Technically Challenging Percutaneous Interventions of Chronic Total Occlusions Are Associated with Enhanced Platelet Activation / Illési Ádám, Fejes Zsolt, Pócsi Marianna, Debreceni Ildikó Beke, Hodosi Katalin, Nagy Jr. Béla, Kappelmayer János, Kőszegi Zsolt, Csanádi Zoltán, Szük Tibor
Dátum:	2023
ISSN:	2077-0383
Megjegyzések:	Percutaneous coronary intervention (PCI) is a frequently performed treatment option for recanalization in patients with chronic total occlusion (CTO). As CTO-PCIs are often complicated and challenging for interventionalists, the stressful and damaging nature of the procedure can be remarkable, thus platelets can be easily activated. Our aim was to investigate the effect of CTO-PCI on platelet activation and the expression of selected circulating microRNAs (miR) of platelet and endothelium origin after CTO-PCI. In this study, 50 subjects after CTO-PCI were enrolled. Blood samples were obtained before PCI, at 2 days and 3?6 months after the procedure to measure the degree of platelet activation and the level of plasma miR-223, miR-181b, and miR-126. Patients were divided based on the characteristics of the intervention. Patients with higher Japanese CTO scores and longer duration of PCI showed significantly elevated platelet P-selectin positivity (p = 0.004 and p = 0.013, respectively) 2 days after the procedure compared to pre-PCI and increased concentration of soluble P-selectin 3?6 months after the intervention (higher Japanese CTO score: p = 0.028 and longer duration of PCI: p = 0.023) compared to baseline values. Shorter total stent length caused a significantly lower miR-181b expression at 3?6 months after the intervention (p = 0.031), while no difference was observed in miR-223 and miR-126. One stent thrombosis occurred during the follow-up period. Although these technically challenging CTO-PCIs may cause enhanced platelet activation right after the intervention and long-term endothelial cell dysfunction, these interventions are not associated with more adverse clinical events.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk biomarker chronic total coronary occlusion coronary intervention microRNA platelet activation
Megjelenés:	Journal of Clinical Medicine. - 12 : 21 (2023), p. 1-14. -
További szerzők:	Fejes Zsolt (1988-) (molekuláris biológus) Pócsi Marianna (1989-) (klinikai laboratóriumi kutató) Bekéné Debreceni Ildikó (1970-) (biológus) Hódosi Katalin Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos) Kappelmayer János (1960-) (laboratóriumi szakorvos) Kőszegi Zsolt (1962-) (kardiológus, belgyógyász) Csanádi Zoltán (1960-) (kardiológus) Szűk Tibor (1967-) (kardiológus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00043 GINOP ÚNKP-22-4-II-DE-120 Egyéb
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001-es BibID:	BIBFORM134812
035-os BibID:	(scopus)105016268170 (wos)001571366900001
Első szerző:	Pócsi Marianna (klinikai laboratóriumi kutató)
Cím:	Comparison of the Effect of CFTR Modulators elexacaftor/tezacaftor/ivacaftor and lumacaftor/ivacaftor via Serum Human Epididymis Protein 4 Concentration in p.Phe508del-CFTR Homozygous Cystic Fibrosis Patients / Pócsi Marianna, Fila Libor, Péterfia Csaba, Halász Adrien, Szanto Tibor G., Mészáros Beáta, Major Judit, Laki István, Szabó Hajnalka, Panyi György, Balogh István, Amaral Margarida D., Macek Jr. Milan, Nagy Jr. Béla
Dátum:	2025
ISSN:	2077-0383
Megjegyzések:	Elevated human epididymis protein 4 (HE4) levels decreased in patients with CF (pwCF) in response to CFTR-specific drugs and negatively correlated with FEV1% predicted values (ppFEV1). Objectives: Although elexacaftor/tezacaftor/ivacaftor (ETI, Kaftrio?) demonstrates more substantial effectiveness than lumacaftor/ivacaftor (LUM/IVA, Orkambi?) in pwCF, plasma biomarkers have not been used to compare treatment efficacy. Hence, our aim was to correlate the change in HE4 levels and the clinical effects of these CFTR modulators (CFTRm). Methods: Serum HE4 concentrations were measured in a total of 123 pwCF homozygous for the p.Phe508del-CFTR variant before treatment and 1?6 months after either ETI or LUM/IVA administration. A correlation between serum HE4 and ppFEV1 was assessed using the Spearman test. HE4 protein levels were also analyzed in the supernatants of p.Phe508del-CFTR CFBE 41o- cells before and after treatment with these CFTRm, and their direct effect on CFTR function was monitored by the whole-cell patch-clamp technique. Results: Serum HE4 levels were reduced below baseline after 3 months of either ETI or LUM/IVA (mean delta HE4: ?38.5 vs. ?18.5 pmol/L, respectively) when the mean change of ppFEV1 was 13.6 vs. 1.6% and remained decreased up to 6 months. A significant inverse correlation between HE4 and ppFEV1 was observed in both study cohorts (r = ?0.537 and r = ?0.575, respectively; p < 0.0001). In agreement with ex vivo results, the effect on p.Phe508del-CFTR was more pronounced by ETI than LUM/IVA in CFBE cells, showing a larger improvement in p.Phe508del-CFTR function and reductions in HE4 levels at 24 h. Conclusions: Serum HE4 negatively correlates with lung function improvement and monitors better drug efficacy in pwCF under ETI than LUM/IVA.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk cystic fibrosis HE4 CFTR modulator ppFEV1 biomarker treatment efficacy
Megjelenés:	Journal of Clinical Medicine. - 14 : 17 (2025), p. 1-18. -
További szerzők:	Fila, Libor Péterfia Csaba Halász Adrienn Szántó Gábor Tibor (1980-) (vegyész) Mészáros Beáta (1985-) (molekuláris biológus, mikrobiológus) Major Judit Laki István Szabó Hajnalka Panyi György (1966-) (biofizikus) Balogh István (1972-) (molekuláris biológus, genetikus) Amaral, Margarida D. Macek Jr., Milan Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos)
Pályázati támogatás:	FK 135327 OTKA UID/04046/2025 Egyéb 00064203/6003 Egyéb
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001-es BibID:	BIBFORM083114
Első szerző:	Szilágyi Bernadett
Cím:	Role of sepsis modulated circulating microRNAs / Szilágyi Bernadett, Fejes Zsolt, Pócsi Marianna, Kappelmayer János, Nagy Béla Jr.
Dátum:	2019
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Journal of the International Federation of Clinical Chemistry and Laboratory Medicine. - 30 : 2 (2019), p. 128-145. -
További szerzők:	Fejes Zsolt (1988-) (molekuláris biológus) Pócsi Marianna (1989-) (klinikai laboratóriumi kutató) Kappelmayer János (1960-) (laboratóriumi szakorvos) Nagy Béla Jr. (1980-) (labordiagnosztikai szakorvos)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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