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1.

001-es BibID:BIBFORM118504
035-os BibID:(WoS)000785275200001 (Scopus)85128172419
Első szerző:Bali Krisztina
Cím:Novel Lineage of Infectious Bronchitis Virus from Sub-Saharan Africa Identified by Random Amplification and Next-Generation Sequencing of Viral Genome / Krisztina Bali, Eszter Kaszab, Szilvia Marton, Seydou Hamadou Hamdiou, Reza Karim Bentaleb, István Kiss, Vilmos Palya, Krisztián Bányai
Dátum:2022
ISSN:2075-1729
Megjegyzések:Avian infectious bronchitis (IB) is among the major viral respiratory and reproductive diseases of chickens caused by Avian coronavirus. In the African continent, IB was first described in countries located in the Mediterranean basin. In other parts of the continent, the epidemiological situation of IB remains unclear. In this study, the complete genome sequences of five IBV strains, originating from the sub-Saharan area were determined. Phylogenetic analysis based on the full-length S1 sequences identified three lineages (GI-14, GI-16, and GI-19) common in Africa and revealed that a strain, D2334/11/2/13/CI, isolated in Ivory Coast may represent a novel lineage within genotype GI. The maximum inter- and intragenotype sequence identities between this strain and other IBVs were 67.58% and 78.84% (nucleotide) and 64.44% and 78.6% (amino acid), respectively. The whole-genome nucleotide identity of the novel variant shared the highest values with a reference Belgian nephropathogenic strain (B1648, 92.4%) and with another study strain from Ivory Coast (D2334/12/2/13/CI, 94.6%). This study illustrates the importance of epidemiological monitoring of IBV in sub-Saharan Africa, as the area may serve as a focal point for newly emerging viral lineages.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Life. - 12 : 4 (2022), p. 1-12. -
További szerzők:Kaszab Eszter (1989-) (biológus) Marton Szilvia Hamdiou, Seydou Hamadou Bentaleb, Reza Karim Kiss István Palya Vilmos Bányai Krisztián (virológus)
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2.

001-es BibID:BIBFORM118494
035-os BibID:(WoS)000643797400001 (Scopus)85103862538
Első szerző:Bali Krisztina
Cím:Recombination Events Shape the Genomic Evolution of Infectious Bronchitis Virus in Europe / Krisztina Bali, Ádám Bálint, Attila Farsang, Szilvia Marton, Borbála Nagy, Eszter Kaszab, Sándor Belák, Vilmos Palya, Krisztián Bányai
Dátum:2021
ISSN:1999-4915
Megjegyzések:Infectious bronchitis of chicken is a high morbidity and mortality viral disease affecting the poultry industry worldwide; therefore, a better understanding of this pathogen is of utmost importance. The primary aim of this study was to obtain a deeper insight into the genomic diversity of field infectious bronchitis virus (IBV) strains using phylogenetic and recombination analysis. We sequenced the genome of 20 randomly selected strains from seven European countries. After sequencing, we created a genome sequence data set that contained 36 European origin field isolates and 33 vaccine strains. When analyzing these 69 IBV genome sequences, we identified 215 recombination events highlighting that some strains had multiple recombination breaking points. Recombination hot spots were identified mostly in the regions coding for non-structural proteins, and multiple recombination hot spots were identified in the nsp2, nsp3, nsp8, and nsp12 coding regions. Recombination occurred among different IBV genotypes and involved both field and vaccine IBV strains. Ninety percent of field strains and nearly half of vaccine strains showed evidence of recombination. Despite the low number and the scattered geographical and temporal origin of whole-genome sequence data collected from European Gammacoronaviruses, this study underlines the importance of recombination as a major evolutionary mechanism of IBVs.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Viruses-Basel. - 13 : 4 (2021), p. 1-13. -
További szerzők:Bálint Ádám Farsang Attila Marton Szilvia Nagy Borbála Kaszab Eszter (1989-) (biológus) Belák Sándor Palya Vilmos Bányai Krisztián (virológus)
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3.

001-es BibID:BIBFORM117940
035-os BibID:(WoS)001139768900001 (Scopus)85181965425
Első szerző:Bálint Ádám
Cím:Spatiotemporal Distribution of PRRSV-1 Clades in Hungary with a Focus on the Era of Disease Eradication / Ádám Bálint, Szilvia Jakab, Eszter Kaszab, Szilvia Marton, Krisztián Bányai, Sándor Kecskeméti, István Szabó
Dátum:2024
ISSN:2076-2615
Megjegyzések:Porcine reproductive and respiratory syndrome (PRRS) is the cause of the most severe economic losses in the pig industry worldwide. PRRSV is extremely diverse in Europe, which poses a significant challenge to disease control within a country or any region. With the combination of phylogenetic reconstruction and network analysis, we aimed to uncover the major routes of the dispersal of PRRSV clades within Hungary. In brief, by analyzing >2600 ORF5 sequences, we identified at least 12 clades (including 6 clades within lineage 1 and 3 clades within lineage 3) common in parts of Western Europe (including Denmark, Germany and the Netherlands) and identified 2 novel clades (designated X1 and X2). Of interest, some genetic clades unique to other central European countries, such as the Czech Republic and Poland, were not identified. The pattern of PRRSV clade distribution is consistent with the route of the pig trade among countries, showing that most of the identified clades were introduced from Western Europe when fatteners were transported to Hungary. As a result of rigorous implementation of the national eradication program, the swine population was declared officially free from PRRSV. This map of viral diversity and clade distribution will serve as valuable baseline information for the maintenance of PRRSV-free status in the post-eradication era.
Tárgyszavak:Orvostudományok Egészségtudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
disease eradication
genetic diversity
molecular epidemiology
phylogeny
porcine reproductive and respiratory syndrome
PRRS
Megjelenés:Animals. - 14 : 1 (2024), p. 1-8. -
További szerzők:Jakab Szilvia Kaszab Eszter (1989-) (biológus) Marton Szilvia Bányai Krisztián (virológus) Kecskeméti Sándor Szabó István
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4.

001-es BibID:BIBFORM118499
035-os BibID:(WoS)000676260800001 (Scopus)85108940755
Első szerző:Fehér Enikő (molekuláris biológus, mikrobiológus)
Cím:Genomic Diversity of CRESS DNA Viruses in the Eukaryotic Virome of Swine Feces / Enikő Fehér, Eszter Mihalov-Kovács, Eszter Kaszab, Yashpal S. Malik, Szilvia Marton, Krisztián Bányai
Dátum:2021
ISSN:2076-2607
Megjegyzések:Replication-associated protein (Rep)-encoding single-stranded DNA (CRESS DNA) viruses are a diverse group of viruses, and their persistence in the environment has been studied for over a decade. However, the persistence of CRESS DNA viruses in herds of domestic animals has, in some cases, serious economic consequence. In this study, we describe the diversity of CRESS DNA viruses identified during the metagenomics analysis of fecal samples collected from a single swine herd with apparently healthy animals. A total of nine genome sequences were assembled and classified into two different groups (CRESSV1 and CRESSV2) of the Cirlivirales order (Cressdnaviricota phylum). The novel CRESS DNA viral sequences shared 85.8-96.8% and 38.1-94.3% amino acid sequence identities for the Rep and putative capsid protein sequences compared to their respective counterparts with extant GenBank record. Data presented here show evidence for simultaneous infection of swine herds with multiple novel CRESS DNA viruses, including po-circo-like viruses and fur seal feces-associated circular DNA viruses. Given that viral genomes with similar sequence and structure have been detected in swine fecal viromes from independent studies, investigation of the association between presence of CRESS DNA viruses and swine health conditions seems to be justified.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Microorganisms. - 9 : 7 (2021), p. 1-13. -
További szerzők:Mihalov-Kovács Eszter Kaszab Eszter (1989-) (biológus) Malik, Yashpal Singh Marton Szilvia Bányai Krisztián (virológus)
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5.

001-es BibID:BIBFORM117251
035-os BibID:(Scopus)85178361033 (WoS)001122834800001
Első szerző:Gál Bence
Cím:A Snapshot on the Genomic Epidemiology of Turkey Reovirus Infections, Hungary / Bence Gál, Renáta Varga-Kugler, Katalin Ihász, Eszter Kaszab, Szilvia Farkas, Szilvia Marton, Vito Martella, Krisztián Bányai
Dátum:2023
ISSN:2076-2615
Megjegyzések:Reovirus infections in turkeys are associated with arthritis and lameness. Viral genome sequence data are scarce, which makes an accurate description of the viral evolution and epidemiology difficult. In this study, we isolated and characterized turkey reoviruses from Hungary. The isolates were identified in 2016; these isolates were compared with earlier Hungarian turkey reovirus strains and turkey reoviruses isolated in the 2010s in the United States. Gene-wise sequence and phylogenetic analyses identified the cell-receptor binding protein and the main neutralization antigen, ?C, to be the most conserved. The most genetically diverse gene was another surface antigen coding gene, ?B. This gene was shown to undergo frequent reassortment among chicken and turkey origin reoviruses. Additional reassortment events were found primarily within members of the homologous turkey reovirus clade. Our data showed evidence for low variability among strains isolated from independent outbreaks, a finding that suggests a common source of turkey reoviruses in Hungarian turkey flocks. Given that commercial vaccines are not available, identification of the source of these founder virus strains would permit a more efficient prevention of disease outbreaks before young birds are settled to fattening facilities.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Avian orthoreovirus
interspecies transmission
phylogenetic analysis
reassortment
Spinareoviridae
whole-genome sequencing
Megjelenés:Animals. - 13 : 22 (2023), p. 1-12. -
További szerzők:Varga-Kugler Renáta Ihász Katalin Kaszab Eszter (1989-) (biológus) Farkas Szilvia Marton Szilvia Martella, Vito Bányai Krisztián (virológus)
Pályázati támogatás:RRF-2.3.1-21-2022-00001
Egyéb
TKP2021-EGA-01
Egyéb
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6.

001-es BibID:BIBFORM130701
035-os BibID:(WoS)001432493900001 (Scopus)85219531336
Első szerző:Homonnay Zalán G.
Cím:Genomic Mosaicism in Fowl Adenovirus 3 Strains / Zalán Homonnay, Szilvia Jakab, Szilvia Marton, Marianna Domán, Krisztina Bali, Eszter Kaszab, Gábor Kemenesi, Tamás Mató, István Kiss, Vilmos Palya, Krisztián Bányai
Dátum:2025
ISSN:2076-2615
Megjegyzések:Fowl adenovirus D is the main cause of inclusion body hepatitis in chickens. Whole genome sequencing was carried out to enrich the genomic sequence database using field isolates of FAdV-D. Out of 44 newly determined genomes, 43 were classified into FAdV-2/-11 and 2 into FAdV-3; no FAdV-9 was identified. Whole-genome based phylogeny showed that FAdV-3 was more distantly related to FAdV 9 and FAdV-2/-11 than FAdV-9 and FAdV-2/-11 to each other. Whole-genome sequence homology analysis revealed that the full-length FAdV-3 genome harbored a ~12 kbp fragment of the genome that shared moderate sequence homology with representative strains of other FAdV-D serotypes but high relatedness with only the FAdV-3 strain whose full-genome is available in GenBank. A closer look onto the fiber and the penton genes of our FAdV-3 isolate identified putative recombination events; both the fiber and the penton coding genes shared fragments originating from FAdV-9. Of interest, ORF19 displayed a close relationship with the homologous genomic region of some FAdV-E strains (amino acid sequence homology, up to 82%). Thus, although FAdV-3 is classified into FAdV-D, the genomic structure of FAdV-3 appears to result from multiple heterotypic and heterologous recombination events. This study highlights the unique origin of FAdV-3.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Animals. - 15 : 4 (2025), p. 1-10. -
További szerzők:Jakab Szilvia Marton Szilvia Domán Marianna (1987-) (biológus) Bali Krisztina Kaszab Eszter (1989-) (biológus) Kemenesi Gábor Mató Tamás Kiss István (Budapest) Palya Vilmos Bányai Krisztián (virológus)
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7.

001-es BibID:BIBFORM118513
035-os BibID:(WoS)000898136200001 (Scopus)85143892810
Első szerző:Jakab Szilvia
Cím:Corrigendum: Genetic diversity of imported PRRSV-2 strains, 2005-2020, Hungary / Szilvia Jakab, Eszter Kaszab, Szilvia Marton, Krisztián Bányai, Ádám Bálint, Imre Nemes, István Szabó
Dátum:2022
ISSN:2297-1769
Megjegyzések:Porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) remains sporadic in Europe. In this study, we investigated the molecular epidemiology of PRRSV-2 infections encompassing 15 years in Hungary. Partial (423 bp long) ORF5 sequences (n = 44) from 20 Hungarian pig herds were analyzed. The study strains fell into two genetic lineages, L1 and L5, being L5 strains more prevalent (88.6 vs. 11.4%). Pairwise sequence identities within Hungarian representative PRRSV-2 strains ranged between 84.7 to 100% (nucleotide, nt) and 85 to 100% (amino acid, aa). When compared with reference strains, identity values fell between 87 and 100% (L1, nt 87?91%, aa 87?93%, reference strain IAF-exp91; L5, nt 87?100%, aa 88?100%, reference strain Ingelvac MLV). Epidemiologic examination implied that the majority of L5 strains were imported repeatedly from other European countries where Ingelvac MLV was approved for routine use. The emergence of L1 strains was thought to be associated with a single introduction and subsequent dissemination between pig farms of a large integrator. Results presented here contribute to a better understanding of the epizootiology of PRRSV-2 infections and shed light on the genetic diversity of viral strains in non-endemic countries.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Veterinary Science. - 9 (2022), p. 1093884. -
További szerzők:Kaszab Eszter (1989-) (biológus) Marton Szilvia Bányai Krisztián (virológus) Bálint Ádám Nemes Imre Szabó István
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8.

001-es BibID:BIBFORM118507
035-os BibID:(WoS)000876193300001 (Scopus)85140402684
Első szerző:Jakab Szilvia
Cím:Genetic diversity of imported PRRSV-2 strains, 2005-2020, Hungary / Szilvia Jakab, Eszter Kaszab, Szilvia Marton, Krisztián Bányai, Ádám Bálint, Imre Nemes, István Szabó
Dátum:2022
ISSN:2297-1769
Megjegyzések:Porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) remains sporadic in Europe. In this study, we investigated the molecular epidemiology of PRRSV-2 infections encompassing 15 years in Hungary. Partial (423 bp long) ORF5 sequences (n = 44) from 20 Hungarian pig herds were analyzed. The study strains fell into two genetic lineages, L1 and L5, being L5 strains more prevalent (88.6 vs. 11.4%). Pairwise sequence identities within Hungarian representative PRRSV-2 strains ranged between 84.7 to 100% (nucleotide, nt) and 85 to 100% (amino acid, aa). When compared with reference strains, identity values fell between 87 and 100% (L1, nt 87-91%, aa 87-93%, reference strain IAF-exp91; L5, nt 87-100%, aa 88-100%, reference strain Ingelvac MLV). Epidemiologic examination implied that the majority of L5 strains were imported repeatedly from other European countries where Ingelvac MLV was approved for routine use. The emergence of L1 strains was thought to be associated with a single introduction and subsequent dissemination between pig farms of a large integrator. Results presented here contribute to a better understanding of the epizootiology of PRRSV-2 infections and shed light on the genetic diversity of viral strains in non-endemic countries.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Frontiers in Veterinary Science. - 9 (2022), p. 1-10. -
További szerzők:Kaszab Eszter (1989-) (biológus) Marton Szilvia Bányai Krisztián (virológus) Bálint Ádám Nemes Imre Szabó István
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9.

001-es BibID:BIBFORM130700
035-os BibID:(WoS)001393473800001 (Scopus)85214488990
Első szerző:Kaszab Eszter (biológus)
Cím:Near-Complete Avipoxvirus Genome Assembled from Skin Lesions of Dead Eurasian Crane (Grus grus) / Kaszab, Eszter; Sós, Endre; Bali, Krisztina; Sós-Koroknai, Viktória; Perge, Edina; Ursu, Krisztina; Marton, Szilvia; Hoitsy, Márton; Kemenesi, Gábor; Bányai, Krisztián
Dátum:2024
ISSN:2076-2615
Megjegyzések:Avian pox is a globally spread viral disease affecting a wide spectrum of wild and domesticated bird species. The disease is caused by a diverse group of large DNA viruses, namely, avipoxviruses (genus Avipoxvirus, family Poxviridae). In this study, gross pathological examination and histopathological examination of skin lesions and several organs suggested acute poxvirus infection of a Eurasian crane (Grus grus, Linnaeus, 1758). Avipoxvirus infection was confirmed by testing wart-like lesions via gene-specific PCR assay and sequencing the obtained amplicon. Phylogenetic analysis of the gene encoding the DNA polymerase revealed that the crane poxvirus clustered in clade A, subclade A3. A large fragment of the poxvirus genome (306,477 bp in length) was assembled from the DNA of a skin specimen. Our study reaffirms previous findings that even complex virus genomes can be determined from a metagenomic assemblage generated directly from avian tissue samples without prior virus isolation, a promising approach for the epidemiologic surveillance of avipoxvirus infections in wild birds and domestic poultry.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Animals. - 15 : 1 (2024), p. 1-13. -
További szerzők:Sós Endre Bali Krisztina Sós-Koroknai Viktória (állatorvos) Perge Edina Ursu Krisztina Marton Szilvia Hoitsy Márton Kemenesi Gábor Bányai Krisztián (virológus)
Pályázati támogatás:RRF-2.3.1-21-2022-00001
Egyéb
RRF-2.3.1-21-2022-00010
Egyéb
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10.

001-es BibID:BIBFORM118492
035-os BibID:(WoS)000697112000009 (Scopus)85101616297
Első szerző:Kaszab Eszter (biológus)
Cím:Genomic evolution of avian polyomaviruses with a focus on budgerigar fledgling disease virus / Eszter Kaszab, Szilvia Marton, Károly Erdélyi, Krisztián Bányai, Enikő Fehér
Dátum:2021
ISSN:1567-1348
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Infection Genetics and Evolution. - 90 (2021), p. 1-7. -
További szerzők:Marton Szilvia Erdélyi Károly Bányai Krisztián (virológus) Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus)
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11.

001-es BibID:BIBFORM118491
035-os BibID:(WoS)000536901100001 (Scopus)85085869043
Első szerző:Kaszab Eszter (biológus)
Cím:Molecular epidemiology and phylodynamics of goose haemorrhagic polyomavirus / Eszter Kaszab, Szilvia Marton, Ádám Dán, Attila Farsang, Ádám Bálint, Krisztián Bányai, Enikő Fehér
Dátum:2020
ISSN:1865-1674 1865-1682
Tárgyszavak:Természettudományok Biológiai tudományok magyar nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Transboundary and Emerging Diseases. - 67 : 6 (2020), p. 2602-2608. -
További szerzők:Marton Szilvia Dán Ádám Farsang Attila Bálint Ádám Bányai Krisztián (virológus) Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus)
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12.

001-es BibID:BIBFORM118531
035-os BibID:(Scopus)85084007052 (WoS)000530731300010
Első szerző:Kaszab Eszter (biológus)
Cím:Occurrence and genetic diversity of CRESS DNA viruses in wild birds : a Hungarian study / eszter Kaszab, György Lengyel, Szilvia Marton, Ádám Dán, Krisztián Bányai, Enikő fehér
Dátum:2020
ISSN:2045-2322
Megjegyzések:Circoviruses, cycloviruses and other circular, replication-associated protein-encoding single stranded (CRESS) DNA viruses have been detected in a variety of animal taxa. In this study, cloacal swab samples (n?=?90) were examined for CRESS DNA viruses from 31 wild bird species living at various aquatic sites in Hungary to identify possible reservoirs of viruses pathogenic to domestic poultry. A total of 30 (33.3%) specimens tested positive with pan-CRESS DNA virus specific PCR. Goose circovirus (GoCV), Duck associated cyclovirus 1 (DuACyV-1) and Garrulus glandarius associated circular virus 1 (GgaCV-1) were detected in nine, three and two different bird species, respectively. Selected specimens were subjected to whole genome sequencing. The obtained sequence data revealed conserved gene structure within the identified virus species and detected homologous (within GoCV) and possible heterologous recombination (within DuACyV-1) events. Results presented here provide new information on the genomic diversity and evolution of selected CRESS DNA viruses.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Scientific Reports. - 10 : 1 (2020), p. 1-10. -
További szerzők:Lengyel György Marton Szilvia Dán Ádám Bányai Krisztián (virológus) Fehér Enikő (1981-) (molekuláris biológus, mikrobiológus)
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