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001-es BibID:	BIBFORM005973
Első szerző:	Damjanovich Sándor (biofizikus)
Cím:	Distribution and mobility of murine histocompatibility H-2Kk antigen in the cytoplasmic membrane / S. Damjanovich, L. Trón, J. Szöllösi, R. Zidovetzki, W. L. Vaz, F. Regateiro, D. J. Arndt-Jovin, T. M. Jovin
Dátum:	1983
Megjegyzések:	The topographical distributions and mobilities of the murine histocompatibility antigen H-2Kk and of concanavalin A (Con A) binding sites have been studied on a murine lymphoma cell line. The spatial distribution of H-2Kk antigens, the average distance between H-2Kk antigens and Con A binding sites, and the separation of different determinants on the H-2Kk antigen itself were determined by using fluorescence resonance energy-transfer measurements with a dual-laser flow sorter. From the lack of energy transfer between bound monoclonal anti-H-2Kk antibodies conjugated with fluorescein (donor) and rhodamine (acceptor), we conclude that the H-2Kk antigen exists without appreciable clustering on the cell surface. Substantial energy transfer between appropriately labeled Con A and antibodies bound to the H-2Kk antigen shows that the two populations are interspersed. Donor/acceptor pairs of monoclonal antibodies binding to different determinants on the same H-2Kk antigen exhibited a degree of energy transfer indicative of a mean separation of 8.6 nm between the sites. Time-resolved phosphorescence anisotropy measurements with anti-H-2Kk antibodies labeled with eosin or erythrosin yielded rotational mobility information for the antigen-antibody complexes on the cell membrane. The rotational correlation time of 10-20 mus and the finite residual anisotropy are compatible with an uniaxial mode of rotation of monomeric antigen around its transmembrane portion and, thus, provide additional evidence for an unclustered distribution. Capping by rabbit anti-mouse IgG immobilized the antigen-antibody complex. Fluorescence recovery after photobleaching was used to calculate an apparent lateral diffusion coefficient of 5 +/- 3 X 10(-10) cm2 . s-1 for the H-2Kk antigen labeled with fluoresceinated IgG or its corresponding Fab fragment.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animal Binding Sites Cell Line Cell Membrane Concanavalin A Diffusion Energy Transfer Fluorescein Fluorescence Fluorescent Antibody Technique Histocompatibility Antigens immunology Kinetics Lymphoma Major Histocompatibility Complex Mice Mice,Inbred Strains Neoplasms,Experimental Receptors,Concanavalin A Rotation Support,Non-U.S.Gov't
Megjelenés:	Proceedings of the National Academy of Sciences of the United States of America. - 80 : 19 (1983), p. 5985-5989. -
További szerzők:	Trón Lajos (1941-) (biofizikus) Szöllősi János (1953-) (biofizikus) Zidovetzki, R. Vaz, W. L. Regateiro, F. Arndt-Jovin, Donna J. Jovin, Thomas M.
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001-es BibID:	BIBFORM004925
Első szerző:	Damjanovich Sándor (biofizikus)
Cím:	Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells / Damjanovich, S., Vereb, G., Schaper, A., Jenei, A., Matko, J., Starink, J. P., Fox, G. Q., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:	1995
Megjegyzések:	Major histocompatibility complex (MHC) class I antigens in the plasma membranes of human T (HUT-102B2) and B (JY) lymphoma cells were probed by immunochemical reagents using fluorescence, transmission electron, and scanning force microscopies. Fluorescent labels were attached to monoclonal antibodies W6/32 or KE-2 directed against the heavy chain of HLA class I (A, B, C) and L368 or HB28 against the beta 2-microglobulin light chain. The topological distribution in the nanometer range was studied by photobleaching fluorescence resonance energy transfer (pbFRET) on single cells. A nonrandom codistribution pattern of MHC class I molecules was observed over distances of 2-10 nm. A second, nonrandom, and larger-scale topological organization of the MHC class I antigens was detected by indirect immunogold labeling and imaging by transmission electron microscopy (TEM) and scanning force microscopy (SFM). Although some differences in antigen distribution between the B- and T-cell lines were detected by pbFRET, both cell lines exhibited similar clustering patterns by TEM and SFM. Such defined molecular distributions on the surfaces of cells of the immune system may reflect an underlying specialization of membrane lipid domains and fulfill important functional roles in cell-cell contacts and signal transduction.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adult analysis beta 2-Microglobulin Cell Line Cell Membrane chemistry Energy Transfer Fluorescence Gold Colloid Histocompatibility Antigens Class I Human Immunohistochemistry immunology Light Lymphocytes Major Histocompatibility Complex methods Microscopy Microscopy,Electron Signal Transduction Support,Non-U.S.Gov't Tumor Cells,Cultured ultrastructure egyetemen (Magyarországon) készült közlemény
Megjelenés:	Proceedings of the National Academy of Sciences of the United States of America. - 92 : 4 (1995), p. 1122-1126. -
További szerzők:	Vereb György (1965-) (biofizikus, orvos) Schaper, Achim Jenei Attila (1966-) (biofizikus) Matkó János (1952-) (biológus) Starink, J. Pascual Fox, Geoffrey Q. Arndt-Jovin, Donna J. Jovin, Thomas M.
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001-es BibID:	BIBFORM006016
Első szerző:	Szöllősi János (biofizikus)
Cím:	Fluorescence energy transfer measurements on cell surfaces : a critical comparison of steady-state fluorimetric and flow cytometric methods / János Szöllősi, Lajos Trón, Sándor Damjanovich, Stephen H. Helliwell, Donna Arndt-Jovin, Thomas M. Jovin
Dátum:	1984
Megjegyzések:	The energy transfer efficiency E was measured between fluorescein-conjugated concanavalin A (Con A) and rhodamine-conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady-state fluorimeter methods were compared. The data were found to be in close agreement after careful correction of the steady-state fluorimetric measurements for contributions from dissociating ligand. The biological variability of the individual cells with respect to E was calculated using an error propagation analysis and were found to be less than the variability in the absolute amount of ligand binding per cell. FCET has a number of advantages over the fluorimetric measurements using suspensions of cells: (1) relatively labile receptor-ligand complexes can be measured; (2) the analysis can be restricted to undamaged cells by gating the data collection on the light-scattering signals; (3) heterogeneous populations of cells with respect to donor and acceptor topology can be distinguished by the correlation of E with other cellular parameters derived from additional signals or combinations thereof; and (4) the dynamics of donor-acceptor redistribution on subpopulations can be measured.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animal Cell Line Cell Membrane Concanavalin A Energy Transfer Flow Cytometry Fluorescence Lymphoma Mathematics methods Mice Mice,Inbred Strains Microscopy,Fluorescence pathology physiology physiopathology Support,Non-U.S.Gov't
Megjelenés:	Cytometry. - 5 : 2 (1984), p. 210-216. -
További szerzők:	Trón Lajos (1941-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Helliwell, Stephen H. Arndt-Jovin, Donna J. Jovin, Thomas M.
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001-es BibID:	BIBFORM006021
Első szerző:	Trón Lajos (biofizikus)
Cím:	Flow cytometric measurement of fluorescence resonance energy transfer on cell surfaces. Quantitative evaluation of the transfer efficiency on a cell-by-cell basis / Trón, L., Szöllösi, J., Damjanovich, S., Helliwell, S. H., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:	1984
Megjegyzések:	A method has been developed for the determination of the efficiency (E) of the fluorescence resonance energy transfer between moieties on cell surfaces by use of a computer-controlled flow cytometer capable of dual wavelength excitation. The absolute value of E may be calculated on a single-cell basis. The analysis requires the measurement of samples stained with donor and acceptor conjugated ligands alone as well as together. In model experiments HK 22 murine lymphoma cells labeled with fluorescein-conjugated concanavalin A (Con A) and/or rhodamine conjugated Con A were used to determine energy transfer histograms. Using the analytic solution to energy transfer in two dimensions, a high surface density of Con A binding sites was found that suggests that the Con A receptor sites on the cell surface are to a degree preclustered . We call this technique flow cytometric energy transfer ( FCET ).
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animal Binding Sites Cell Line Cell Membrane Comparative Study Concanavalin A Energy Transfer Flow Cytometry Fluorescein Fluorescein-5-isothiocyanate Fluoresceins Fluorescence Ligands Lymphoma metabolism Mice Rhodamines Spectrometry,Fluorescence Support,Non-U.S.Gov't Thiocyanates
Megjelenés:	Biophysical Journal. - 45 : 5 (1984), p. 939-946. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Helliwell, Stephen H. Arndt-Jovin, Donna J. Jovin, Thomas M.
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