Találati lista | Tudóstér

001-es BibID:	BIBFORM125597
035-os BibID:	(scopus)85207761844 (wos)001348191500001
Első szerző:	Benziane, Anass (molekuláris biológus)
Cím:	Synergistic effect of folic acid and hypericin administration to improve the efficacy of photodynamic therapy via folate receptors / Benziane Anass, Huntosová Veronika, Pevná Viktória, Zauska Lubos, Vámosi György, Hovan Andrej, Zelenková Gabriela, Zelenák Vladimír, Almási Miroslav
Dátum:	2024
ISSN:	1011-1344 1873-2682
Megjegyzések:	Transport systems are developed to improve the solubility of the transported drug, increase its stability, enhance its pharmacological activity and target cancer while minimising side effects. In this work, nanoporous silica particles that can be functionalized and loaded with a large number of hydrophobic molecules are proposed. The designed system was modified with folic acid to target the folic acid receptors of cancer cells. This modification enabled a higher uptake of the drug by the cells. Hypericin was selected as a hydrophobic molecule/drug with photodynamic properties suitable for diagnosis and therapy. Fluorescence microscopy and flow cytometry were used to detect the targeting and distribution of hypericin in the cancer cells. Furthermore, the combination of folic acid and hypericin has been shown to form singlet oxygen and to have a synergistic effect in improving the efficacy of photodynamic therapy. The functionalisation of the particles proposed in this work holds great potential for the delivery of hydrophobic drugs to other types of cancer cells with increased expression of the folic acid receptor to which the particles can be attached.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Mesoporous silica SBA-15 Folate receptors Targeting Hypericin Photodynamic therapy Transport
Megjelenés:	Journal Of Photochemistry And Photobiology B-Biology. - 261 (2024), p. 1-14. -
További szerzők:	Huntošová, Veronika Pevná, Viktória Zauška, Ľuboš Vámosi György (1967-) (biofizikus) Hovan, Andrej Zelenková, Gabriela Zeleňák, Vladimír Almáši, Miroslav
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM129250
035-os BibID:	(scopus)105001325615 (wos)001460543600001
Első szerző:	Huntošová, Veronika
Cím:	The potential of metal-organic framework MIL-101(Al)-NH2 in the forefront of antiviral protection of cells via interaction with SARS-CoV-2 spike RBD protein and their antibacterial action mediated with hypericin and photodynamic treatment / Huntosová Veronika, Benziane Anass, Zauska Lubos, Ambro Lubos, Olejárová Sona, Joniová Jaroslava, Hlávková Nina, Wagnieres Georges, Zelenková Gabriela, Diko Pavel, Bednarcík Jozef, Zákány Florina, Kovács Tamás, Sedlák Erik, Vámosi György, Almási Miroslav
Dátum:	2025
ISSN:	0021-9797
Megjegyzések:	The global pandemic of SARS-CoV-2 has highlighted the necessity for innovative therapeutic solutions. This research presents a new formulation utilising the metal?organic framework MIL-101(Al)?NH2, which is loaded with hypericin, aimed at addressing viral and bacterial challenges. Hypericin, recognised for its antiviral and antibacterial efficacy, was encapsulated to mitigate its hydrophobicity, improve bioavailability, and utilise its photodynamic characteristics. The MIL-101(Al)?NH2 Hyp complex was synthesised, characterised, and evaluated for its biological applications for the first time. The main objective of this study was to demonstrate the multimodal potential of such a construct, in particular the effect on SARS-CoV-2 protein levels and its interaction with cells. Both in vitro and in vivo experiments demonstrated the effective transport of hypericin to cells that express ACE2 receptors, thereby mimicking mechanisms of viral entry. In addition, hypericin found in the mitochondria showed selective phototoxicity when activated by light, leading to a decrease in the metabolic activity of glioblastoma cells. Importantly, the complex also showed antibacterial efficacy by selectively targeting Gram-positive Staphylococcus epidermidis compared to Gram-negative Escherichia coli under photodynamic therapy (PDT) conditions. To our knowledge, this study was the first to demonstrate the interaction between hypericin, MIL-101(Al)?NH2 and the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein, which inhibits cellular uptake and colocalises with ACE2-expressing cells. Therefore, the dual functionality of the complex ? targeting the viral RBD and the antibacterial effect via PDT ? emphasises its potential to mitigate complications of viral infections, such as secondary bacterial infections. In summary, these results suggest that MIL-101(Al)?NH2 Hyp is a promising multifunctional therapeutic agent for antiviral and antibacterial applications, potentially contributing to the improvement of COVID-19 treatment protocols and the treatment of co-infections.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk MIL-10l(Al)-NH2 Hypericin ACE2 receptors RBD spike protein Photodynamic therapy Selectivity
Megjelenés:	Journal Of Colloid And Interface Science. - 691 (2025), p. 1-18. -
További szerzők:	Benziane, Anass (1990-) (molekuláris biológus) Zauška, Ľuboš Ambro, Lubos Olejárová, Sona Joniová, Jaroslava Hlávková, Nina Wagnieres, Georges Zelenková, Gabriela Diko, Pavel Bednarcík, Jozef Zákány Florina (1989-) (általános orvos) Kovács Tamás (1985-) (általános orvos) Sedlák Erik Vámosi György (1967-) (biofizikus) Almáši, Miroslav
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM116504
035-os BibID:	(scopus)85170688343 (wos)001081473700001
Első szerző:	Pevná, Viktória
Cím:	Effective transport of aggregated hypericin encapsulated in SBA-15 nanoporous silica particles for photodynamic therapy of cancer cells / Pevná Viktória, Zauska Lubos, Benziane Anass, Vámosi György, Girman Vladimír, Miklósová Monika, Zelenák Vladimír, Huntosová Veronika, Almási Miroslav
Dátum:	2023
ISSN:	1011-1344 1873-2682
Megjegyzések:	Photodynamic therapy (PDT) represents an interesting modality for the elimination of damaged biomaterials and cells. This treatment takes advantage of the photosensitizing properties of molecules that are active only when irradiated with light. In the present work, a dual property of hypericin, a hydrophobic molecule with high performance in photodiagnostics and photodynamic therapy, was exploited. The non-fluorescent and photodynamically inactive form of hypericin aggregates was loaded into the nanopores of SBA-15 silica particles. The synthesized particles were characterized by infrared spectroscopy, thermogravimetry, differential thermal analysis, small-angle X-ray scattering and transmission electron microscopy. Hypericin aggregates were confirmed by absorption spectra typical of aggregated hypericin and by its short fluorescence lifetime. Release of hypericin from the particles was observed toward serum proteins, mimicking physiological conditions. Temperature- and time-dependent uptake of hypericin by cancer cells showed gradual release of hypericin from the particles and active cellular transport by endocytosis. A closer examination of SBA-15-hypericin uptake by fluorescence lifetime imaging showed that aggregated hypericin molecules, characterized by a short fluorescence lifetime (?4 ns), were still present in the SBA-15 particles upon uptake by cells. However, monomerization of hypericin in cancer cells was observed by extending the hypericin fluorescence lifetime by ?8 ns, preferentially in lipid compartments and the plasma membrane. This suggests a promising prognosis for delayed biological activity of the entire cargo, which was confirmed by effective PDT in vitro. In summary, this work presents an approach for safe, inactive delivery of hypericin that is activated at the target site in cells and tissues.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Nanoporous silica Hypericin Transport Cancer cells Fluorescence lifetime Photodynamic therapy
Megjelenés:	Journal Of Photochemistry And Photobiology B-Biology. - 247 (2023), p. 1-11. -
További szerzők:	Zauška, Ľuboš Benziane, Anass (1990-) (molekuláris biológus) Vámosi György (1967-) (biofizikus) Girman, Vladimír Miklóšová, Monika Zeleňák, Vladimír Huntošová, Veronika Almáši, Miroslav
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: