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001-es BibID:	BIBFORM049817
Első szerző:	Csernoch László (élettanász)
Cím:	Qγ and Ca release flux in skeletal muscle fibers / L. Csernoch, I. Uribe, M. Rodríguez, G. Pizarro, E. Ríos
Dátum:	1989
ISSN:	0006-3495
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:	Biophysical Journal. - 55 (1989), p. 85a. -
További szerzők:	Uribe, I. Rodríguez, M. Pizzaro, Gonzalo Rios, E.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM040115
Első szerző:	Csernoch László (élettanász)
Cím:	The elementary events of Ca2+ release elicited by membrane depolarization in mammalian muscle / Csernoch, L., Zhou J., Stern M. D., Brum G., Rios E.
Dátum:	2004
ISSN:	0022-3751
Megjegyzések:	Cytosolic [Ca(2+)] transients elicited by voltage clamp depolarization were examined by confocal line scanning of rat skeletal muscle fibres. Ca(2+) sparks were observed in the fibres' membrane-permeabilized ends, but not in responses to voltage in the membrane-intact area. Elementary events of the depolarization-evoked response could be separated either at low voltages (near -50 mV) or at -20 mV in partially inactivated cells. These were of lower amplitude, narrower and of much longer duration than sparks, similar to 'lone embers' observed in the permeabilized segments. Their average amplitude was 0.19 and spatial half-width 1.3 microm. Other parameters depended on voltage. At -50 mV average duration was 111 ms and latency 185 ms. At -20 mV duration was 203 ms and latency 24 ms. Ca(2+) release current, calculated on an average of events, was nearly steady at 0.5-0.6 pA. Accordingly, simulations of the fluorescence event elicited by a subresolution source of 0.5 pA open for 100 ms had morphology similar to the experimental average. Because 0.5 pA is approximately the current measured for single RyR channels in physiological conditions, the elementary fluorescence events in rat muscle probably reflect opening of a single RyR channel. A reconstruction of cell-averaged release flux at -20 mV based on the observed distribution of latencies and calculated elementary release had qualitatively correct but slower kinetics than the release flux in prior whole-cell measurements. The qualitative agreement indicates that global Ca(2+) release flux results from summation of these discrete events. The quantitative discrepancies suggest that the partial inactivation strategy may lead to events of greater duration than those occurring physiologically in fully polarized cells.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Physiology-London. - 557 : Pt1 (2004), p. 43-58. -
További szerzők:	Zhou, Jia Stern, M. D. Brum, G. Rios, E.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM049820
Első szerző:	Pizzaro, Gonzalo
Cím:	Tetracaine and pathways of Ca2+ release in skeletal muscle / G. Pizarro, L. Csernoch, I. Uribe, E. Ríos
Dátum:	1989
ISSN:	0006-3495
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idézhető absztrakt
Megjelenés:	Biophysical Journal. - 55 (1989), p. 237a. -
További szerzők:	Csernoch László (1961-) (élettanász) Uribe, I. Rios, E.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM040109
Első szerző:	Pizzaro, Gonzalo
Cím:	Differential effects of tetracaine on two kinetic components of calcium release in frog skeletal muscle fibres / Pizarro, G., Csernoch, L., Uribe, I., Rios, E.
Dátum:	1992
ISSN:	0022-3751
Megjegyzések:	1. Intramembrane charge movements and changes in intracellular calcium concentration were recorded simultaneously in voltage clamped cut skeletal muscle fibres of the frog in the presence and absence of tetracaine. 2. Extracellular application of 20 microM tetracaine reduced the increase in myoplasmic [Ca2+]. The effect on the underlying calcium release flux from the sarcoplasmic reticulum was to suppress the peak of the release while sparing the steady level attained at the end of 100 ms clamp depolarizations. 3. While the peak of the release flux at corresponding voltages was reduced by 62% after the addition of tetracaine, the rate of inactivation was the same when the pulses elicited release fluxes of similar amplitude. 4. Higher concentrations of tetracaine, 0.2 mM, abolished the calcium signal in stretched fibres whereas in slack fibres this concentration left a non-inactivating calcium release flux. 5. Lowering the extracellular pH antagonized the effect of the drug both on charge movements and on calcium signals. The permanently charged analogue tetracaine methobromide lacked effects on excitation-contraction coupling. 6. These results imply that the two kinetic components of calcium release flux have very different tetracaine sensitivities. They are also consistent with an intracellular site of action of the drug at low concentration. Taken together they strongly suggest that the inactivating and non-inactivating components of calcium release correspond to different pathways: one that inactivates, is sensitive to tetracaine and is controlled by calcium, and another that does not inactivate, is much less sensitive to tetracaine and is directly controlled by voltage.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Physiology. - 457 (1992), p. 525-538. -
További szerzők:	Csernoch László (1961-) (élettanász) Uribe, I. Rios, E.
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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