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001-es BibID:	BIBFORM132437
035-os BibID:	(Scopus)105015659007 (WoS)001567521000001
Első szerző:	Benhamza, Ibtissem (Biologist)
Cím:	H2A.Z-nucleosomes are stabilized by the superhelicity-dependent DNA binding of the C-terminal tail of the histone variant / Ibtissem Benhamza, Laszlo Imre, Zutao Yu, Peter Pal ifj. Nanasi, Pialy Sen, Kata Nora Enyedi, Katalin Goda, György Vamosi, Gabor Szabo
Dátum:	2025
ISSN:	1949-1034 1949-1042
Megjegyzések:	Using an in situ nucleosome stability assay based on salt extraction, we identified distinct stability features of H2A.Z-containing nucleosomes linked to alternative interactions of the histone variant's Cterminal tail (Imre et al., Nat. Commun., 2024). In DT40 cells expressing either full-length or Cterminally truncated human H2A.Z1, we show that nucleosome stability is tail-dependent also through the spectacles of intercalator sensitivity, raising the possibility that the tail may bind to DNA in a superhelicity-dependent fashion. Supporting this, fluorescence correlation spectroscopy detected binding of a fluorescent H2A.Z-tail nonapeptide to supercoiled?but not relaxed?plasmid DNA, while a scrambled peptide showed negligible binding. The DNA topology-dependent binding of the unstructured H2A.Z C-terminus, by affecting nucleosome stability, may be of functional significance in various roles of the histone variant, demonstrating the strong interplay between DNA topology and nucleosome stability and exemplifying how it may be exploited by the cell for regulatory purposes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk H2A.Z nucleosome stability superhelicity C-terminal tail
Megjelenés:	Nucleus. - 16 : 1 (2025), p. 1-14. -
További szerzők:	Imre László (1979-) (biológus) Yu, Zutao Nánási Péter Pál ifj. (1987-) (sejtbiológus) Sen, Pialy (1996-) Enyedi Kata Nóra (1987-) (vegyész) Goda Katalin (1969-) (biofizikus) Vámosi György (1967-) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Pályázati támogatás:	K138524 OTKA ANN 135107 OTKA K146028 OTKA ERA-Net NEURON 2024-1.2.2-ERA_NET-2024?00009 Egyéb
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001-es BibID:	BIBFORM099098
Első szerző:	Káldy Jenő
Cím:	Effects of Hydrostatic Pressure Treatment of Newly Fertilized Eggs on the Ploidy Level and Karyotype of Pikeperch Sander lucioperca (Linnaeus, 1758) / Káldy Jenő, Patakiné Várkonyi Eszter, Fazekas Georgina Lea, Nagy Zoltán, Sándor Zsuzsanna J., Bogár Katalin, Kovács Gyula, Molnár Mariann, Lázár Bence, Goda Katalin, Gyöngy Zsuzsanna, Ritter Zsuzsanna, Nánási Péter, Horváth Ákos, Ljubobratović Uroŝ
Dátum:	2021
ISSN:	2075-1729
Megjegyzések:	We studied the effect of different magnitudes (7000 PSI (48.26 MPa), 8000 PSI (55.16 MPa), and 9000 PSI (62.05 MPa)) of hydrostatic pressure on the ploidy of pikeperch larvae. Pressure shock was applied 5 min after the fertilization of eggs at a water temperature of 14.8 ? 1 ?C. A 7000 PSI pressure shock was applied for 10 or 20 min, while 8000 and 9000 PSI treatments lasted for 10 min. Each treatment with its respective control was completed in triplicate, where different females' eggs served as a replicate. In the treatment groups exposed to 7000 PSI for 10 min, only diploid and triploid larvae were identified, while 2n/3n mosaic individuals were found after a 20-min exposure to a 7000 PSI pressure shock. The application of 8000 or 9000 PSI pressure shocks resulted in only triploid and mosaic individuals. Among larvae from eggs treated with 8000 PSI, three mosaic individuals with 2n/3n karyotype were identified (4.0 ? 6.9%), while a single (2.0 ? 3.5%) 1n/3n mosaic individual was found in the 9000 PSI-treated group. To our knowledge, this is the first report that demonstrates the induction of a haplo-triploid karyotype by hydrostatic pressure shock in teleost fish. The dominance of triploid individuals with a reasonable survival rate (36.8 ? 26.1%) after 8000 PSI shock supports the suitability of the hydrostatic pressure treatment of freshly fertilized eggs for triploid induction in pikeperch.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk triploid mosaic karyotype pressure duration chromosome number PSI
Megjelenés:	Life. - 11 : 12 (2021), p. 1-11. -
További szerzők:	Patakiné Várkonyi Eszter Fazekas Georgina Nagy Zoltán Jakabné Sándor Zsuzsanna Bogár Katalin Kovács Gyula Molnár Marianna Lázár Bence Goda Katalin (1969-) (biofizikus) Gyöngy Zsuzsanna (1991-) (molekuláris biológus) Ritter Zsuzsanna Nánási Péter Pál ifj. (1987-) (sejtbiológus) Horváth Ákos Ljubobratović, Uroš
Pályázati támogatás:	K124815 OTKA
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