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1.

001-es BibID:BIBFORM129151
035-os BibID:(Scopus)105001011774 (WoS)001452091200001
Első szerző:Géczi Dóra (biotechnológus)
Cím:Identification of Deregulated miRNAs and mRNAs Involved in Tumorigenesis and Detection of Glioblastoma Patients Applying Next-Generation RNA Sequencing / Dóra Géczi, Álmos Klekner, István Balogh, András Penyige, Melinda Szilágyi, József Virga, Andrea Bakó, Bálint Nagy, Bernadett Torner, Zsuzsanna Birkó
Dátum:2025
ISSN:1424-8247
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Pharmaceuticals. - 18 : 3 (2025), p. 1-29. -
További szerzők:Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Virga József (1989-) Bakó Andrea (1979-) (orvos) Nagy Bálint (1956-) (molekuláris genetikus) Torner Bernadett (1997-) (molekuláris biológus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002 of the "National Brain Research Program NAP 2.0
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2.

001-es BibID:BIBFORM094352
035-os BibID:(scopus)85105433955 (wos)000662004700001
Első szerző:Géczi Dóra (biotechnológus)
Cím:Analysis of Circulating miRNA Profile in Plasma Samples of Glioblastoma Patients / Dóra Géczi, Bálint Nagy, Melinda Szilágyi, András Penyige, Álmos Klekner, Adrienn Jenei, József Virga, Zsuzsanna Birkó
Dátum:2021
ISSN:1661-6596 1422-0067
Megjegyzések:Background: Glioblastoma multiforme (GBM) is among the most aggressive cancers with a poor prognosis. Treatment options are limited, clinicians lack efficient prognostic and predictive markers. Circulating miRNAs?besides being important regulators of cancer development?may have potential as diagnostic biomarkers of GBM. (2) Methods: In this study, profiling of 798 human miRNAs was performed on blood plasma samples from 6 healthy individuals and 6 patients with GBM, using a NanoString nCounter Analysis System. To validate our results, five miRNAs (hsa-miR-433-3p, hsa-miR-362-3p, hsa-miR-195-5p, hsa-miR-133a-3p, and hsa-miR-29a-3p) were randomly chosen for RT-qPCR detection. (3) Results: In all, 53 miRNAs were significantly differentially expressed in plasma samples of GBM patients when data were filtered for FC 1 and FDR 0.1. Target genes of the top 39 differentially expressed miRNAs were identified, and we carried out functional annotation and pathway enrichment analysis of target genes via GO and KEGG-based tools. General and cortex-specific protein?protein interaction networks were constructed from the target genes of top miRNAs to assess their functional connections. (4) Conclusions: We demonstrated that plasma microRNA profiles are promising diagnostic and prognostic molecular biomarkers that may find an actual application in the clinical practice of GBM, although more studies are needed to validate our results.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
glioblastoma
circulating miRNA
blood plasma
network analysis
biomarker
NanoString
Megjelenés:International Journal Of Molecular Sciences. - 22 : 10 (2021), p. 1-20. -
További szerzők:Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus) Penyige András (1954-) (molekuláris genetikus) Klekner Álmos (1970-) (idegsebész) Jenei Adrienn (1978-) (biológus, kémikus) Virga József (1989-) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002
NKP
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3.

001-es BibID:BIBFORM128685
Első szerző:Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:Általános és humángenetika / Birkó Zuzsa, Biró Sándor, Fehér Zsigmond, Penyige András, Schlammadinger József, Sipiczki Mátyás, Szeszák Ferenc, Szentesiné Szirák Krisztina, Vargha György, Vitális Sándor
Dátum:2003
Megjelenés:Debrecen : DEOEC Elnöki Hivatal, 2003
Terjedelem:864 p.
Tárgyszavak:Természettudományok Biológiai tudományok szakkönyv
könyv
További szerzők:Biró Sándor (1949-) (molekuláris genetikus) Fehér Zsigmond (1949-) (molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus) Schlammadinger József (1938-) (kutató orvos) Sipiczki Mátyás (1948-) (biológus) Szeszák Ferenc Szirák Krisztina (1973-) (molekuláris genetikus) Vargha György (1951-) (orvos) Vitális Sándor (1933-) (kutatóorvos)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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4.

001-es BibID:BIBFORM009630
Első szerző:Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:Lack of A-factor production induces the expression of nutrient scavenging and stress-related proteins in Streptomyces griseus / Birkó Zsuzsanna, Swiatek Magdalena, Szájli Emília, Medzihradszky F. Katalin, Vijgenboom Erik, Penyige András, Keserű Judit, van Wezel Gilles P., Biró Sándor
Dátum:2009
ISSN:1535-9476 (Print)
Megjegyzések:The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule for the soil-inhabiting streptomycetes. Starvation is a major trigger for development,and nutrients are provided by degradation of the vegetative mycelium via a process of programmed cell death, reusing proteins, nucleic acids, and cell wall material. The A-factor regulon includes many extracellular hydrolases. Here we show via proteomics analysis that many nutrientscavenging and stress-related proteins were overexpressed in an A-factor non-producing mutant of Streptomyces griseus B-2682. Transcript analysis showed that this is primarily due to differential transcription of the target genes during early development. The targets include proteins relating to nutrient stress and environmental stress and an orthologue of the Bacillus sporulation control protein Spo0M. The enhanced expression of these proteins underlines the stress that is generated by the absence of A-factor. Wild-type developmental gene expression was restored to the A-factor non-producing mutant by the signaling protein Factor C in line with our earlier observation that Factor C triggers A-factor production.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
C-faktor
Streptomyces griseus
proteomika
Megjelenés:Molecular and Cellular Proteomics : MCP - 8 : 10 (2009), p. 2396-2403. -
További szerzők:Swiatek, Magdalena Szájli Emília Medzihradszky-Fölkl Katalin Vijgenboom, Erik Penyige András (1954-) (molekuláris genetikus) Keserű Judit (1976-) (molekuláris genetikus) Wezel, Gilles P., van Biró Sándor (1949-) (molekuláris genetikus)
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elektronikus változat
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5.

001-es BibID:BIBFORM001540
Első szerző:Hádáné Birkó Zsuzsanna (molekuláris genetikus)
Cím:The secreted signaling protein factor C triggers the A-factor response regulon in streptomyces griseus : overlapping signaling routes / Birkó Z., Bialek S., Buzás K., Szájli E., Traag B. A., Medzihradszky K. F., Rigali S., Vijgenboom E., Penyige A., Kele Z., van Wezel G. P., Bíró S.
Dátum:2007
Megjegyzések:Members of the prokaryotic genus Streptomyces produce over 60% of all known antibiotics and a wide range of industrial enzymes. A leading theme in microbiology is which signals are received and transmitted by these organisms to trigger the onset of morphological differentiation and antibiotic production. The small -butyrolactone A-factor is an important autoregulatory signaling molecule in streptomycetes, and A-factor mutants are blocked in development and antibiotic production. In this study we showed that heterologous expression of the 324-amino acid secreted regulatory protein Factor C resulted in restoration of development and enhanced antibiotic production of an A-factor-deficient bald mutant of Streptomyces griseus, although the parental strain lacks an facC gene. Proteome analysis showed that in the facC transformant the production of several secreted proteins that belong to the A-factor regulon was restored. HPLC-MS/MS analysis indicated that this was due to restoration of A-factor production to wild-type levels in the transformant. This indicates a connection between two highly divergent types of signaling molecules and possible interplay between their regulatory networks.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Streptomyces szignalizáció
Megjelenés:Molecular and Cellular Proteomics : MCP - 6 : 7 (2007), p. 1248-1256. -
További szerzők:Bialek, Sylwia Buzás Krisztina Szájli Emília Traag, Bjorn A. Medzihradszky-Fölkl Katalin Rigali, Sebastien Vijgenboom, Erik Kele Zoltán Wezel, Gilles P., van Biró Sándor (1949-) (molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus)
Internet cím:elektronikus változat
DOI
elektronikus változat
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6.

001-es BibID:BIBFORM073381
035-os BibID:(WoS)000489312608202
Első szerző:Keserű Judit (molekuláris genetikus)
Cím:Determination of miR-196a Single Nucleotide Polymorphism (SNP) with melting-curve analysis in the population of patients with ovarian cancer / J. S. Keserű, J. Lukács, B. Soltész, K. Szirák, Z. Birkó, A. Penyige, B. Nagy, R. Póka
Dátum:2018
ISSN:1018-4813 1476-5438
Megjegyzések:Introduction: miRNA molecules are short, non-coding sequences regulating gene expression after transcription. They are important in the development, progression and treatability of tumours. Single Nucleotide Polymorphism (SNP) is the most frequent type of genetic polymorphism. That is true also for miRNAs and their polymorphisms can cause alterations in the gene expression profile. miR-196a was also linked to the genesis of different tumours.Aim: Search for correlation between miR-196a polymorphism and development of ovarian cancer.Materials and Methods: 75 patients with ovarian cancer and 75 healthy persons were investigated. 15-16 mL blood anticoagulated with EDTA was drawn. DNA was isolated with silica absorption method and the melting curve of PCR products generated with LightSnip kit (TibMolbiol, Berlin, Germany) developed for miR-196a (rs11614913) SNP was determined by LightCycler 96 equipment. Allele and genotype frequencies were specified and Student t-test was applied for statistical analysis of data.Results: The Tm of PCR products were 55.5?C for T allele and 62.6?C for C allele with melting-curve analysis. T allele occurred in 32.66% in population of patients and in 40.66% in control group. Genotypes among control persons were 18.66% for TT, 44.0% for TC, and 37.33% CC, while in case of patients these frequencies were 12.0%, 41.33%, and 46.66%, respectively (p=0.3815).Conclusion: miR-196a influences the expression of 684 genes, it requires further complex investigation, whether it is involved in the development of ovarian cancer.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
folyóiratcikk
ovarian cancer
miR-196a
SNP
Megjelenés:European Journal of Human Genetics. - 26 : Suppl. (2018), p. 1. -
További szerzők:Lukács János (1975-) (szülész-nőgyógyász, genetikus) Soltész Beáta (1987-) (molekuláris biológus) Szirák Krisztina (1973-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Penyige András (1954-) (molekuláris genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Póka Róbert (1960-) (szülész-nőgyógyász, klinikai onkológus)
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7.

001-es BibID:BIBFORM108635
035-os BibID:(Scopus)85148964475
Első szerző:Márton Éva (biológus)
Cím:Comparative Analysis of Transcriptomic Changes including mRNA and microRNA Expression Induced by the Xenoestrogens Zearalenone and Bisphenol A in Human Ovarian Cells / Éva Márton, Alexandra Varga, András Penyige, Zsuzsanna Birkó, István Balogh, Bálint Nagy, Melinda Szilágyi
Dátum:2023
ISSN:2072-6651
Megjegyzések:Xenoestrogens are natural or synthetic compounds that mimic the effect of endogenous estrogens and might cause cancer. We aimed to compare the global transcriptomic response to zearalenone (ZEA; mycotoxin) and bisphenol A (BPA; plastic additive) with the effect of physiological estradiol (E2) in the PEO1 human ovarian cell line by mRNA and microRNA sequencing. Estrogen exposure induced remarkable transcriptomic changes: 308, 288 and 63 genes were upregulated (log2FC > 1); 292, 260 and 45 genes were downregulated (log2FC < ?1) in response to E2 (10 nM), ZEA (10 nM) and BPA (100 nM), respectively. Furthermore, the expression of 13, 11 and 10 miRNAs changed significantly (log2FC > 1, or log2FC < ?1) after exposure to E2, ZEA and BPA, respectively. Functional enrichment analysis of the significantly differentially expressed genes and miRNAs revealed several pathways related to the regulation of cell proliferation and migration. The effect of E2 and ZEA was highly comparable: 407 genes were coregulated by these molecules. We could identify 83 genes that were regulated by all three treatments that might have a significant role in the estrogen response of ovarian cells. Furthermore, the downregulation of several miRNAs (miR-501- 5p, let-7a-2-3p, miR-26a-2-3p, miR-197-5p and miR-582-3p) was confirmed by qPCR, which might support the proliferative effect of estrogens in ovarian cells.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
xenoösztrogén
zearalenon
mikotoxin
biszfenol A
petefészekrák
transzkriptomika
mikroRNS
RNS szekvenálás
Megjelenés:Toxins. - 15 : 2 (2023), p. 1-22. -
További szerzők:Beke-Varga Alexandra Edit (1994-) (molekuláris biológus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Balogh István (1972-) (molekuláris biológus, genetikus) Nagy Bálint (1956-) (molekuláris genetikus) Szilágyi Melinda (1984-) (biológus)
Pályázati támogatás:FK138021
OTKA
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8.

001-es BibID:BIBFORM136487
035-os BibID:(scopus)105032156153 (wos)001707066300001
Első szerző:Torner Bernadett (molekuláris biológus)
Cím:The role of miRNAs in the development of brain metastases originating from lung adenocarcinoma / Torner Bernadett, Klekner Álmos, Balogh István, Penyige András, Géczi Dóra, Gáspár Tekla, Geszti Gréta, Birkó Zsuzsanna
Dátum:2026
ISSN:1664-8021
Megjegyzések:Introduction: Brain metastases (BMs) represent most malignant lesions of the central nervous system. Lung cancer-particularly lung adenocarcinoma (LUAD, ?25%)-is the most common source of BMs. MicroRNAs (miRNAs) play a crucial role in regulating gene expression, thereby contributing to tumor progression and metastatic spread. Identifying these regulatory molecules may enable a deeper understanding of the mechanisms driving LUAD brain metastasis (LUAD-BM) development and reveal therapeutic targets to prevent or limit disease progression. Methods: Next-generation RNA sequencing (RNA-seq) was performed on six LUAD-BM and six non-tumorous human brain tissue samples to assess miRNA expression profiles. Additionally, RNA-seq data from 20 primary LUAD and 15 normal lung tissue samples were obtained from The Cancer Genome Atlas (TCGA) database. MiRNAs showing the most pronounced alterations in LUAD-BM samples were selected for validation by real time quantitative polymerase chain reaction (RT-qPCR). Results: Analysis of RNA-seq data identified 229 differentially expressed (DE) miRNAs between LUAD-BM and control samples. Functional annotation analysis indicated that these DE miRNAs are key regulators of tumorigenesis and metastasis. Using the Mann-Whitney U test, ten miRNAs were confirmed to differ significantly between LUAD-BM and normal brain tissue. Receiver operating characteristic (ROC) curve analysis demonstrated their diagnostic potential. Among the ten validated miRNAs, miR-200c-3p, miR-146b-5p, and miR-3934-5p showed distinct expression patterns between primary LUAD and LUAD-BM, while miR-10a-5p, miR-210-3p, and miR-130b-3p exhibited stepwise dysregulation along the normal lung-LUAD-LUAD-BM axis, suggesting their involvement in metastatic progression. Conclusion: We identified ten miRNAs that showed preliminary ability to differentiate LUAD-BM from normal brain tissue. These findings indicate possible diagnostic and therapeutic implications. Among these, six miRNAs showed significant expression changes along the normal control-primary LUAD-LUAD-BM axis, highlighting their potential as biomarkers and therapeutic targets in BM development.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
biomarker panel
brain tissue
invasion
lung adenocarcinoma brain metastasis
miRNAs
next-generation sequencing
Megjelenés:Frontiers in Genetics. - 17 (2026), p. 1-17. -
További szerzők:Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Géczi Dóra (1989-) (biotechnológus) Gáspár Tekla Geszti Gréta Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
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9.

001-es BibID:BIBFORM128116
035-os BibID:(scopus)85218910548 (wos)001429631100001
Első szerző:Torner Bernadett (molekuláris biológus)
Cím:Construction of a miRNA Panel for Differentiating Lung Adenocarcinoma Brain Metastases and Glioblastoma / Torner Bernadett, Géczi Dóra, Klekner Álmos, Balogh István, Penyige András, Birkó Zsuzsanna
Dátum:2025
ISSN:2072-6694
Megjegyzések:Abstract: Background/Objectives: Brain metastases (BM) are the most common type of intracra-nial malignant tumor and are associated with high mortality. More than 50% of BM cases origi-nate from lung cancer, and lung adenocarcinoma (LUAD) is most commonly associated with the development of BM (25%). The differential diagnosis of solitary BM and glioblastoma (GBM) ? one of the most aggressive and fatal malignant brain tumors ? remains a considerable challenge. Given the major role of microRNAs (miRNAs) in regulating gene expression, their clinical po-tential as biomarkers for tumor diagnosis and prognosis offers significant promise. Methods: Next Generation RNA Sequencing (RNA-seq) was used to assess the miRNA expression profiles of 6 LUAD-BM, 6 GBM, and 6 control (non-tumoral brain tissue samples) human brain tissue samples. miRNAs exhibiting the most significant differential expression in LUAD-BM patients in comparison to both control subjects and GBM patients were selected for validation through RT-qPCR. Results: The analysis of RNA-seq data revealed the presence of 229 differentially ex-pressed miRNAs in the comparison between LUAD-BM and control samples and 46 in the com-parison between LU-AD-BM and GBM samples. Eight miRNAs were selected for further analysis, four of which were upregulated and four downregulated, based on the significant differences in their expression levels observed between the LUAD-BM samples and the other two groups, as confirmed with the Mann-Whitney U test. A functional enrichment analysis was also conducted based on a miRNA-centered target analysis performed using the miRNet tool. To assess the di-agnostic potential of these differentially expressed miRNAs, we performed a receiver operating characteristic (ROC) curve analysis. Conclusions: A panel of eight miRNAs was identified in human brain tissue samples, exhibiting high accuracy in distinguishing LUAD-BM from both GBM and control samples.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
lung adenocarcinoma brain metastasis, glioblastoma
miRNAs
Next-Generation Sequencing
brain tissue
biomarker panel
Megjelenés:Cancers. - 17 : 4 (2025), p. 1-23. -
További szerzők:Géczi Dóra (1989-) (biotechnológus) Klekner Álmos (1970-) (idegsebész) Balogh István (1972-) (molekuláris biológus, genetikus) Penyige András (1954-) (molekuláris genetikus) Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus)
Pályázati támogatás:2017-1.2.1-NKP-2017-00002
Egyéb
Bridging Fund of the Faculty of Medicine, University of Debrecen (IB)
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