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001-es BibID:	BIBFORM002001
Első szerző:	Chodaczek, Grzegorz
Cím:	Iron-mediated dismutation of superoxide anion augments antigen-induced allergic inflammation : effect of lactoferrin / Grzegorz Chodaczek, Alfredo Saavedra-Molina, Attila Bacsi, Marian L. Kruzel, Sanjiv Sur, Istvan Boldogh
Dátum:	2007
Megjegyzések:	The authors previously showed that pollen grain-, pollen grain extract-. and subpollen particle-induced allergic inflammation in lungs and eyes is robustly augmented by their intrinsic NAD(P)H oxidase activity. Here they sought to determine whether lactoferrin (LF), an iron-binding protein and immune modulator, decreases allergic inflammation induced by ragweed (Ambrosia artemisiifolia) pollen grain extract (RWE). MATERIAL/METHODS: The impact of LF on NAD(P)H oxidase in pollen grains and reactive oxygen species (ROS) levels in vitro and in the lungs of experimental animals was assessed by use of redox-sensitive probes and specific inhibitors. The influence of LF on RWE-induced allergic inflammation was determined in a mouse experimental model of asthma. RESULTS: The data show that the intrinsic NAD(P)H oxidase of pollen grains generates superoxide anion (O2-) and that LF does not alter its enzymatic activity, as shown by nitroblue tetrazolium and cytochrome c assays. On the other hand, LF significantly decreased H(2)- O(2)- and lipid peroxide (4-hydroxynoneal and malondialdehyde) levels in airway lining fluids and lung epithelium after intranasal challenge of naive or sensitized mice with RWE. Furthermore, a single dose of LF prevented/decreased the abundance of the RWE-induced robust accumulation of inflammatory and mucin-producing cells in airways and subepithelial compartments and decreased airway hyperreactivity. CONCLUSION: These data suggest that the reduced conversion of NAD(P)H oxidase-generated O(2)- into H(2)- O(2)- and/or OH, which in turn synergistically enhanced pollen antigen-induced airway inflammation, is due to the iron-binding capacity of LF. These results support the utility of LF in human allergic inflammatory disorders.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Lactoferrin allergy airway infl ammation oxidative stress
Megjelenés:	Postepy higieny i medycyny doswiadczalnej (Online). - 61 (2007), p. 268-278. -
További szerzők:	Saavedra-Molina, Alfredo Bácsi Attila (1967-) (immunológus) Kruzel, Marian L. Sur, Sanjiv Boldogh István
Internet cím:	elektronikus változat elektronikus változat
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