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001-es BibID:	BIBFORM002759
Első szerző:	Lidke, Diane S.
Cím:	In vivo imaging using quantum-dot-conjugated probes / Lidke, D. S., Nagy P., Arndt-Jovin, D. J.
Dátum:	2007
Megjegyzések:	This unit describes the use of quantum dots (QDs) for live-cell imaging and the use of QDs in flow cytometry for quantitative analysis of ligand binding constants and receptor density. Conventional fluorophores and visible fluorescent protein (VFP) constructs have allowed visualization of many cellular processes. However, organic and biomolecular fluorophores have limitations in their applications, due to their small Stokes' shift and tendency to photobleach during prolonged imaging. QDs have many advantages over conventional fluorophores, including high brightness and photostability, which make them an exceptional tool for live-cell imaging. There are a large variety of commercially available QDs with different surface reactivities and characteristics. The authors have limited the laboratory protocols presented here to the use of streptavidin-coupled QDs because this gives almost universal applicability to any cell surface receptor by coupling the ligand or antibody that recognizes the receptor to biotin and visualizing the complex by use of QDs
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Animals Antibodies antibody combining site antigen antibody reaction Antigen-Antibody Reactions article Binding Sites Binding Sites,Antibody Biotinylation Cell Culture cell surface receptor Cells,Cultured Dyes dyes,reagents,indicators,markers and buffers Flow Cytometry fluorescence microscopy fluorescent dye Fluorescent Dyes Human Humans Indicators and Reagents instrumentation ligand Ligands metabolism methodology Mexico Mice Microscopy,Fluorescence microsphere Microspheres mouse quantum dot Quantum Dots Receptors,Cell Surface Streptavidin ultrastructure
Megjelenés:	Current Protocols in Cell Biology. - Supplement 36. (2007), unit 25.1.1-25.1.18. -
További szerzők:	Nagy Péter (1971-) (biofizikus) Arndt-Jovin, Donna J.
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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