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001-es BibID:	BIBFORM004137
Első szerző:	Szentesi Péter (élettanász)
Cím:	Altered elementary calcium release events and enhanced calcium release by thymol in rat skeletal muscle / Szentesi P., Szappanos H., Szegedi C., Gonczi M., Jona I., Cseri J., Kovacs L., Csernoch L.
Dátum:	2004
Megjegyzések:	The effects of thymol on steps of excitation-contraction coupling were studied on fast-twitch muscles of rodents. Thymol was found to increase the depolarization-induced release of calcium from the sarcoplasmic reticulum, which could not be attributed to a decreased calcium-dependent inactivation of calcium release channels/ryanodine receptors or altered intramembrane charge movement, but rather to a more efficient coupling of depolarization to channel opening. Thymol increased ryanodine binding to heavy sarcoplasmic reticulum vesicles, with a half-activating concentration of 144M and a Hill coefficient of 1.89, and the open probability of the isolated and reconstituted ryanodine receptors, from 0.090.03 to 0.220.04 at 30M. At higher concentrations the drug induced long-lasting open events on a full conducting state. Elementary calcium release events imaged using laser scanning confocal microscopy in the line-scan mode were reduced in size, 0.920.01 vs. 0.700.01, but increased in duration, 561 vs. 791ms, by 30M thymol, with an increase in the relative proportion of lone embers. Higher concentrations favored long events, resembling embers in control, with duration often exceeding 500ms. These findings provide direct experimental evidence that the opening of a single release channel will generate an ember, rather than a spark, in mammalian skeletal muscle.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Biophysical Journal. - 86 : 3 (2004), p. 1436-1453. -
További szerzők:	Szappanos Henrietta (1976-) (biológus, élettanász) Szegedi Csaba Gönczi Mónika (1974-) (élettanász) Jóna István (1948-) (élettanász, fizikus) Cseri Julianna (1949-2022) (laboratóriumi diagnosztika szakorvos) Kovács László (1939-) (élettanász) Csernoch László (1961-) (élettanász)
Internet cím:	elektronikus változat DOI elektronikus változat
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