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001-es BibID:	BIBFORM004330
Első szerző:	Yoshida, Kaoru
Cím:	Functional implications of membrane modification with semenogelins for inhibition of sperm motility in humans / Yoshida K., Krasznai Z. T., Krasznai Z., Yoshiike M., Kawano N., Yoshida M., Morisawa M., Tóth Z., Bazsáné Kassai Z., Márián T., Iwamoto T.
Dátum:	2009
Megjegyzések:	Semenogelin I and II (Sgs) are the major component of human semen coagulum. The protein is rapidly cleaved after ejaculation by a prostate-specific antigen, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa. Sgs inhibit human sperm motility; however, there is currently no information on its effect on the sperm membrane. This study investigated the role of Sgs on human sperm motility through regulation of membrane potential and membrane permeability. Fresh semen samples were obtained from normozoospermic volunteers, and studies were conducted using motile cells selected using the swim-up method. Sgs changed the characteristics of sperm motion from circular to straightforward as evaluated by a computer-assisted motility analyzer, and all parameters were decreased more than 2.5 mg/mL. The results demonstrate that Sgs treatment immediately hyperpolarized the membrane potential of swim-up-selected sperm, changed the membrane structure, and time-dependently increased membrane permeability, as determined through flow cytometric analysis. The biphasic effects of Sgs were time- and dose-dependent and partially reversible. In addition, a monoclonal antibody against Sgs showed positive binding to cell membrane proteins in fixed cells, observed with confocal fluorescence microscopy. These results demonstrate that Sgs modifies the membrane structure, indirectly inhibiting motility, and provides suggestions for a therapy for male infertility through selection of a functional sperm population using Sgs.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Cell Motility and the Cytoskeleton 66 : 2 (2009), p. 99-108. -
További szerzők:	Krasznai Zoárd Tibor (1973-) (szülész-nőgyógyász, gyermeknőgyógyász) Krasznai Zoltán (1950-) (biofizikus) Yoshiike, Miki Kawano, Natsuko Yoshida, Manabu Morisawa, Masaaki Tóth Zoltán (1948-) (szülész-nőgyógyász, humángenetikus) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Márián Teréz (1950-) (radiobiológus) Iwamoto, Teruaki
Internet cím:	elektronikus változat DOI
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