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001-es BibID:	BIBFORM004633
035-os BibID:	(scopus)0344447088 (wos)000074651400008
Első szerző:	Nagy Péter (biofizikus)
Cím:	Intensity-based energy transfer measurements in digital imaging microscopy / Nagy P., Vamosi G., Bodnar A., Lockett S. J., Szollosi J.
Dátum:	1998
ISSN:	175-7571
Megjegyzések:	Investigation of protein-protein associations is important in understanding structure and function relationships in living cells. Using Forster-type resonance energy transfer between donor and acceptor labeled monoclonal antibodies we can assess the cell surface topology of membrane proteins against which the antibodies were raised. In our current work we elaborated a quantitative image microscopic technique based on the measurement of fluorescence intensities to calculate the energy transfer efficiency on a pixel-by-pixel basis. We made use of the broad excitation and emission spectrum of cellular autofluorescence for background correction of images. In addition to the reference autofluorescence images (UV background) we recorded three fluorescent images (donor, acceptor and energy transfer signal) of donor-acceptor double labeled samples, and corrected for spectral spillage of the directly excited donor and acceptor fluorescence into the energy transfer image. After careful image registration we were able to calculate the energy transfer efficiency on a pixel-by -pixel basis. In this paper, we also present a critical comparison between results obtained with this method and other approaches (photobleaching and flow cytometric energy transfer measurements)
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Antibodies Antibodies, Monoclonal Biophysics Cell Line chemistry Comparative Study Energy Transfer Fluorescence Human Hungary Image Processing, Computer-Assisted immunology Membrane Proteins methods Microscopy Microscopy, Fluorescence Models, Theoretical Proteins Signal Processing,Computer-Assisted Spectrometry, Fluorescence statistics and numerical data Support, Non-U.S.Gov't
Megjelenés:	European Biophysics Journal. - 27 : 4 (1998), p. 377-389. -
További szerzők:	Vámosi György (1967-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Lockett, Steven J. Szöllősi János (1953-) (biofizikus)
Internet cím:	elektronikus változat
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