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001-es BibID:	BIBFORM004648
Első szerző:	Jenei Attila (biofizikus)
Cím:	Picosecond multiphoton scanning near-field optical microscopy / Jenei, A., Kirsch, A. K., Subramaniam, V., Arndt-Jovin, D. J., Jovin, T. M.
Dátum:	1999
Megjegyzések:	We have implemented simultaneous picosecond pulsed two- and three-photon excitation of near-UV and visible absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 1064-nm emission from a pulsed Nd:YVO4 laser was used to excite the visible mitochondrial specific dye MitoTracker Orange CM-H2TMRos or a Cy3-labeled antibody by two-photon excitation, and the UV absorbing DNA dyes DAPI and the bisbenzimidazole BBI-342 by three-photon excitation, in a shared aperture SNOM using uncoated fiber tips. Both organelles in human breast adenocarcinoma cells (MCF 7) and specific protein bands on polytene chromosomes of Drosophila melanogaster doubly labeled with a UV and visible dye were readily imaged without photodamage to the specimens. The fluorescence intensities showed the expected nonlinear dependence on the excitation power over the range of 5-40 mW. An analysis of the dependence of fluorescence intensity on the tip-sample displacement normal to the sample surface revealed a higher-order function for the two-photon excitation compared to the one-photon mode. In addition, the sample photobleaching patterns corresponding to one- and two-photon modes revealed a greater lateral confinement of the excitation in the two-photon case. Thus, as in optical microscopy, two-photon excitation in SNOM is confined to a smaller volume
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Antibodies Cells chemistry Chromosomes Dna Drosophila melanogaster Dyes Fluorescence Fluorescent Dyes genetics Human Lasers metabolism methods Microscopy Microscopy, Fluorescence Mitochondria Photons Support, Non-U.S.Gov't Tumor Cells,Cultured ultrastructure Ultraviolet Rays külföldön készült közlemény
Megjelenés:	Biophysical Journal. - 76 : 2 (1999), p. 1092-1100. -
További szerzők:	Kirsch, Achim K. Subramaniam, Vinod Arndt-Jovin, Donna J. Jovin, Thomas M.
Internet cím:	elektronikus változat DOI
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