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001-es BibID:	BIBFORM004663
035-os BibID:	(scopus)0343962249 (wos)000087812700009
Első szerző:	Gál István (sebész)
Cím:	Protease-elicited TUNEL positivity of non-apoptotic fixed cells / Gal, I., Varga, T., Szilagyi, I., Balazs, M., Schlammadinger, J., Szabo, G.
Dátum:	2000
Megjegyzések:	The appearance of free DNA ends in the chromatin is usually considered an indication of advanced apoptosis. Unexpectedly, the nuclei of non-apoptotic cells derived from mouse thymuses could be specifically labeled by terminal transferase after proteinase K treatment of the fixed, cytocentrifuged samples. Artifactual mechanical or contaminating nucleolytic factors have been ruled out as players in the generation of free DNA ends. The phenomenon was detected in both formaldehyde- and ethanol-fixed specimens, in agarose-embedded fixed cells, and in chromatin spreads. By urea-agarose gel electrophoresis, the average single-strand size of the DNA molecules carrying the free ends was found between 50 and 250 kb. We suggest that ss discontinuities preexisting in the fixed normal cells are unmasked by protease treatment eliciting TUNEL (terminal transferase-mediated nick end-labeling) positivity.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Animals Apoptosis Artifacts Biophysics Cells Chromatin cytology Dna DNA Fragmentation Electrophoresis, Agar Gel Endopeptidase K Formaldehyde Hungary In Situ Nick-End Labeling metabolism Mice Mice, Inbred BALB C physiology Research Support Thymus Gland
Megjelenés:	The Journal of Histochemistry and Cytochemistry. - 48 : 7 (2000), p. 963-970. -
További szerzők:	Varga Tamás (1971-) (biológus) Szilágyi Ildikó (orvos) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Schlammadinger József (1938-) (kutató orvos) Szabó Gábor (1953-) (biofizikus)
Internet cím:	DOI elektronikus változat
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