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001-es BibID:	BIBFORM004740
035-os BibID:	(WOS)000184609400011 (scopus)12444317206
Első szerző:	Szilágyi Ildikó (orvos)
Cím:	Non-random features of loop-size chromatin fragmentation / Szilagyi, I., Varga, T., Szekvolgyi, L., Hegedus, E., Goda, K., Kaczur, V., Bacso, Z., Nakayama, Y., Posafi, J., Pongor, S., Szabo, G.
Dátum:	2003
ISSN:	730-2312 (Print)
Megjegyzések:	Upon isolation of DNA from normal eukaryotic cells by standard methods involving extensive proteolytic treatment, a rather homogeneous population of loop-size, double-stranded DNA fragments is regularly obtained. These DNA molecules can be efficiently end-labeled by the DNA polymerase I Klenow fragment, as well as by a 3'- to -5'-exonuclease-free Klenow enzyme, but not by terminal transferase (TdT) unless the ends have been filled up by Klenow, suggesting that dominantly 5' protruding termini are generated upon fragmentation. The filled-up termini were used for cloning the distal parts of the approximately 50 kb fragments. BLAST analysis of the sequence of several clones allowed us to determine the sequence of the non-cloned side of the breakpoints. Comparison of 25, 600 bp-long breakpoint sequences demonstrated prevalence of repetitive elements. Consensus motives characteristic of the breakpoint sequences have been identified. Several sequences exhibit peculiar computed conformational characteristics, with sharp transition or center of symmetry located exactly at the breakpoint. Our data collectively suggest that chromatin fragmentation involves nucleolytic cleavages at fragile/hypersensitive sites delimiting loop-size fragments in a non-random manner. Interestingly, the sequence characteristics of the breakpoints are reminiscent of certain breakpoint cluster regions frequently subject to gene rearrangements.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animals Base Sequence Biophysics Cells chemistry Chromatin Dna DNA Fragmentation DNA Nucleotidylexotransferase DNA Polymerase I Electrophoresis,Gel,Two-Dimensional Eukaryotic Cells HL-60 Cells Humans Hungary isolation and purification Jurkat Cells methods Mice Nih 3T3 Cells Prevalence Research Sequence Analysis,DNA Support egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal of Cellular Biochemistry. - 89 : 6 (2003), p. 1193-1205. -
További szerzők:	Varga Tamás (1971-) (biológus) Székvölgyi Lóránt (1977-) (biofizikus, biokémikus, sejtbiológus) Hegedűs Éva (1978-) (biofizikus) Goda Katalin (1969-) (biofizikus) Kaczur Viktória Bacsó Zsolt (1963-) (biofizikus) Nakayama, Yuji Pósafi János Pongor Sándor Szabó Gábor (1953-) (biofizikus)
Internet cím:	elektronikus változat DOI
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