Találati lista | Tudóstér

001-es BibID:	BIBFORM004854
Első szerző:	Andrásfalvy Márton
Cím:	The beta subunit of the type I Fcepsilon receptor is a target for peptides inhibiting IgE-mediated secretory response of mast cells / Andrasfalvy, M., Peterfy, H., Toth, G., Matko, J., Abramson, J., Kerekes, K., Vamosi, G., Pecht, I., Erdei, A.
Dátum:	2005
ISSN:	0022-1767
Megjegyzések:	Peptides originally derived from complement component C3a were earlier shown to inhibit the type I FcepsilonR (FcepsilonRI)-mediated degranulation of mucosal type mast cells. In the present study, we show that C3a7, a peptide with a natural sequence, and its modified derivative, C3a9, are powerful inhibitors of the above response of both serosal and mucosal type mastocytes. We demonstrate that these peptides inhibit FcepsilonRI-induced membrane proximal events, suppress phosphorylation of the FcepsilonRI beta subunit, the protein tyrosine kinase Lyn, as well as the transient rise in free cytosolic Ca2+ level. The late phase of cellular response was also inhibited, as demonstrated by the reduced TNF-alpha secretion. Experiments using two independent methods provided evidence that the interaction site of complement-derived peptides is the FcepsilonRI beta-chain. This was further supported by fluorescence confocal microscopic colocalization and resonance energy transfer measurements. Taken together, these results suggest the presence of distinct "activating" and "inhibitory" motifs in the C3a sequence. Response to both is in balance under physiologic conditions. Furthermore, present data predict that such inhibitory peptides may serve as potent agents for future therapeutic intervention.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban 1-Phosphatidylinositol 3-Kinase Animals antagonists & inhibitors Calcium Cells chemistry Complement Complement C3a Energy Transfer Fluorescence Hungary Immunoglobulin E immunology Mast Cells metabolism methods Mice Mice,Inbred BALB C Necrosis Oligopeptides Peptides pharmacology Phosphorylation physiology Protein Subunits Receptors,IgE Research secretion Support Tumor Necrosis Factor-alpha Tyrosine
Megjelenés:	The Journal of Immunology. - 175 : 5 (2005), p. 2801-2806. -
További szerzők:	Péterfy Hajna Tóth Gábor (Szeged) Matkó János (1952-) (biológus) Abramson, Jakub Kerekes Krisztina Vámosi György (1967-) (biofizikus) Pecht, Israel Erdei Anna
Internet cím:	elektronikus változat
Borító:
Saját polcon: