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001-es BibID:	BIBFORM004884
Első szerző:	Rubovszky Bálint (élettanász)
Cím:	Detection of channel proximity by nanoparticle-assisted delaying of toxin binding : a combined patch-clamp and flow cytometric energy transfer study / Rubovszky, B., Hajdu, P., Krasznai, Z., Gaspar, R., Waldmann, T. A., Damjanovich, S., Bene, L.
Dátum:	2005
ISSN:	0175-7571
Megjegyzések:	Gold nanoparticles of 30 nm diameter bound to cell-surface receptor major histocompatibility complex glycoproteins (MHCI and MHCII), interleukin-2 receptor alpha subunit (IL-2Ralpha), very late antigen-4 (VLA-4) integrin, transferrin receptor, and the receptor-type protein tyrosin phosphatase CD45 are shown by the patch-clamp technique to selectively modulate binding characteristics of Pi(2) toxin, an efficient blocker of K(v)1.3 channels. After correlating the electrophysiological data with those on the underlying receptor clusters obtained by simultaneously conducted flow cytometric energy transfer measurements, the modulation was proved to be sensitive to the density and size of the receptor clusters, and to the locations of the receptors as well. Based on the observation that engagement of MHCII by a monoclonal antibody down-regulates channel current and based on the close nanometer-scale proximity of the MHCI and MHCII glycoproteins, an analogous experiment was carried out when gold nanoparticles bound to MHCI delayed down-regulation of the K(v)1.3 current initiated by ligation of MHCII. Localization of K(v)1.3 channels in the nanometer-scale vicinity of the MHC-containing lipid rafts is demonstrated for the first time. A method is proposed for detecting receptor-channel or receptor-receptor proximity by observing nanoparticle-induced increase in relaxation times following concentration jumps of ligands binding to channels or to receptors capable of regulating channel currents.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antibodies Biophysics Cell Line chemistry drug effects Energy Transfer Flow Cytometry Glycoproteins Gold Human Humans Interleukin-2 Interleukin-2 Receptor alpha Subunit Ion Channel Gating Kv1.3 Potassium Channel Ligands Major Histocompatibility Complex methods Nanotubes Particle Size Patch-Clamp Techniques pharmacokinetics physiology Potassium Potassium Channels Potassium Channels,Voltage-Gated Protein Binding Research Scorpion Venoms Support T-Lymphocytes
Megjelenés:	European Biophysics Journal. - 34 : 2 (2005), p. 127-143. -
További szerzők:	Hajdu Péter (1975-) (biofizikus) Krasznai Zoltán (1950-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus) Bene László (1963-) (biofizikus)
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