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001-es BibID:	BIBFORM004939
Első szerző:	Bene László (biofizikus)
Cím:	Major histocompatibility complex class I protein conformation altered by transmembrane potential changes / Bene, L., Szollosi, J., Balazs, M., Matyus, L., Gaspar, R., Ameloot, M., Dale, R. E., Damjanovich, S.
Dátum:	1997
ISSN:	0196-4763
Megjegyzések:	The nature of charge distributions in membrane-bound macromolecular structures renders them susceptible to interaction with transmembrane potential fields. As a result, conformational changes in such species may be expected to occur when this potential is altered. We have detected reversible conformational change in the major histocompatibility complex (MHC) class I antigen in the plasma membrane of human JY cells, as monitored by flow-cytometric resonance energy-transfer, upon reduction of the transmembrane potential (depolarization). This change increased the intramolecular energy-transfer efficiency between fluorescent donor- and acceptor-labeled monoclonal antibodies directed, respectively, to epitopes on the light (beta 2-microglobulin) and the heavy chains of the MHC class I antigen. Repolarization of the depolarized samples restored the energy-transfer efficiency to the original values measured before depolarization. Depolarization caused similar relative changes in fluorescence resonance energy-transfer efficiency when Fab fragments were used for labeling MHC class I complex, suggesting that the observed phenomenon is not restricted to whole monoclonal antibodies.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antibodies, Monoclonal Antigen Presentation B-Lymphocytes beta 2-Microglobulin Cell Membrane chemistry cytology Dyes Energy Transfer Enzyme Activation Flow Cytometry Fluorescein-5-isothiocyanate Fluorescence Fluorescent Dyes Histocompatibility Antigens Class I Human Hungary immunology Light Major Histocompatibility Complex Membrane Potentials metabolism methods Na(+)-K(+)-Exchanging ATPase Patch-Clamp Techniques physiology Protein Conformation Rhodamines Surface Properties
Megjelenés:	Cytometry. - 27 : 4 (1997), p. 353-357. -
További szerzők:	Szöllősi János (1953-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Ameloot, Marcel Dale, Robert E. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:	elektronikus változat
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