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001-es BibID:	BIBFORM004940
Első szerző:	Damjanovich Sándor (biofizikus)
Cím:	Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15 : a fluorescence resonance energy transfer study / Damjanovich, S., Bene, L., Matko, J., Alileche, A., Goldman, C. K., Sharrow, S., Waldmann, T. A.
Dátum:	1997
ISSN:	0027-8424
Megjegyzések:	Assembly and mutual proximities of alpha, beta, and gamma(c) subunits of the interleukin 2 receptors (IL-2R) in plasma membranes of Kit 225 K6 T lymphoma cells were investigated by fluorescence resonance energy transfer (FRET) using fluorescein isothiocyanate- and Cy3-conjugated monoclonal antibodies (mAbs) that were directed against the IL-2R alpha, IL-2R beta, and gamma(c) subunits of IL-2R. The cell-surface distribution of subunits was analyzed at the nanometer scale (2-10 nm) by FRET on a cell-by-cell basis. The cells were probed in resting phase and after coculture with saturating concentrations of IL-2, IL-7, and IL-15. FRET data from donor- and acceptor-labeled IL-2R beta-alpha, gamma-alpha, and gamma-beta pairs demonstrated close proximity of all subunits to each other in the plasma membrane of resting T cells. These mutual proximities do not appear to represent mAb-induced microaggregation, because FRET measurements with Fab fragments of the mAbs gave similar results. The relative proximities were meaningfully modulated by binding of IL-2, IL-7, and IL-15. Based on FRET analysis the topology of the three subunits at the surface of resting cells can be best described by a "triangular model" in the absence of added interleukins. IL-2 strengthens the bridges between the subunits, making the triangle more compact. IL-7 and IL-15 act in the opposite direction by opening the triangle possibly because they associate their private specific alpha receptors with the beta and/or gamma(c) subunits of the IL-2R complex. These data suggest that IL-2R subunits are already colocalized in resting T cells and do not require cytokine-induced redistribution. This colocalization is significantly modulated by binding of relevant interleukins in a cytokine-specific manner.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Adult analysis Cell Membrane chemistry Energy Transfer Fluorescence Human Interleukin-15 Interleukin-2 Interleukin-7 metabolism pharmacology Protein Conformation Receptors,Interleukin-2 T-Lymphocytes Tumor Cells,Cultured
Megjelenés:	Proceedings of the National Academy of Sciences of the United States of America. - 94 : 24 (1997), p. 13134-13139. -
További szerzők:	Bene László (1963-) (biofizikus) Matkó János (1952-) (biológus) Alileche, Abdelkrim Goldman, Caroline K. Sharrow, Susan Waldmann, Thomas A.
Internet cím:	elektronikus változat elektronikus változat
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