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001-es BibID:	BIBFORM004947
Első szerző:	Kiss Erzsébet
Cím:	Effect of TSH and anti-TSH receptor antibodies on the plasma membrane potential of polymorphonuclear granulocytes / Kiss, E., Balazs, C., Bene, L., Damjanovich, S., Matko, J.
Dátum:	1997
ISSN:	0165-2478
Megjegyzések:	Effects of thyrotropin hormone (TSH) and anti-TSH receptor antibodies on the plasma membrane potential of polymorphonuclear granulocytes (PMN) were analyzed by means of flow cytometry. Both TSH and the autoantibody caused a rapid, dose-dependent hyperpolarization of the plasma membrane of PMNs. TSH was also able to mask (revert) the depolarizing effect of a chemotactic peptide, fMLP, on PMNs. No detectable rise in the cytosolic free calcium level accompanied the observed hyperpolarization. Quinine, a blocker of Ca(2+)-activated and voltage-gated K+ channels did not affect the hyperpolarization by TSH and antibodies. Decreasing the [K+] gradient across the plasma membrane by valinomycin, however, blocked the hyperpolarizing effect. Peptide362-376 (derived from the extracellular domain of TSH receptor) also blocked the hyperpolarization induced by both TSH and anti-TSHR antibodies. These data suggest that the observed hyperpolarization is a specific, receptor-mediated early signal during interaction of PMNs with TSH or anti-TSHR antibodies.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Amino Acid Sequence Antibodies,Monoclonal Autoantibodies Calcium Cell Membrane chemistry Dose-Response Relationship,Drug drug effects Flow Cytometry Fluorescence Polarization Human Hungary immunology Membrane Potentials metabolism Molecular Sequence Data N-Formylmethionine Leucyl-Phenylalanine Neutrophils Oligopeptides pharmacology physiology Potassium Potassium Channels Quinine Receptors,Thyrotropin Support,Non-U.S.Gov't Thyrotropin ultrastructure Valinomycin
Megjelenés:	Immunology Letters. - 55 : 3 (1997), p. 173-177. -
További szerzők:	Balázs Csaba Bene László (1963-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Matkó János (1952-) (biológus)
Internet cím:	elektronikus változat DOI
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