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001-es BibID:	BIBFORM005095
Első szerző:	Shiba, K.
Cím:	Na+/Ca2+ exchanger modulates the flagellar wave pattern for the regulation of motility activation and chemotaxis in the ascidian spermatozoa / Shiba, K., Marian, T., Krasznai, Z., Baba, S. A., Morisawa, M., Yoshida, M.
Dátum:	2006
ISSN:	886-1544 (Print)
Megjegyzések:	Ion channels and ion exchangers are known to be important participants in various aspects of sperm physiology, e.g. motility activation, chemotaxis, the maintenance of motility and the acrosome reaction in the sperm. We report here on a role of the K+ -independent Na+/Ca2+ exchanger (NCX) on ascidian sperm. Reverse-transcriptase PCR reveals that the NCX is expressed in the testis while immunoblotting and immunolocalization demonstrate that the NCX exists on the sperm in the ascidian Ciona savignyi and C. intestinalis. A potent blocker of the NCX, KB-R7943 was found to block sperm-activating and -attracting factor (SAAF)-induced motility activation, sperm motility and sperm chemotaxis. We further analyzed the effects of this blocker on motility parameters such as the flagellar waveform, curvature, beat frequency, amplitude and wavelength of the sperm flagella. Inhibition of the NCX caused two distinct effects: a low concentration of KB-R7943 induced symmetric bending, whereas a high concentration of KB-R7943 resulted in asymmetric flagellar bending. These findings suggest that the NCX plays important roles in the regulation of SAAF-induced sperm chemotaxis, motility activation and motility maintenance in the ascidian. This study provides new information toward an understanding of Ca2+ transport systems in sperm motility and chemotaxis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analogs and derivatives Animals antagonists and inhibitors Anti-Arrhythmia Agents Calcium Calcium Channel Blockers Chemotaxis drug effects Immunoblotting Ion Channels Male metabolism pharmacology physiology Research Sodium-Calcium Exchanger Sperm Motility Sperm Tail Spermatozoa Support Thiourea Urochordata
Megjelenés:	Cell Motility and the Cytoskeleton. - 63 : 10 (2006), p. 623-632. -
További szerzők:	Márián Teréz (1950-) (radiobiológus) Krasznai Zoltán (1950-) (biofizikus) Baba, S. A. Morisawa, Masaaki Yoshida, Manabu
Internet cím:	DOI
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