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001-es BibID:	BIBFORM005096
Első szerző:	Szarvas Mariann
Cím:	Differential platelet deposition onto collagen in cone-and-plate and parallel plate flow chambers / Szarvas, M., Oparaugo, P., Udvardy, M. L., Tóth, J., Szántó, T., Daróczi, L., Vereb, G., Hársfalvi, J.
Dátum:	2006
Megjegyzések:	To routinely test the formation of thrombi and the effect of drugs modifying it, proper test systems are needed. Their design should rely on the laws of rheology and the physiology of laminar flow. To best model physiological or pathological shear conditions, parallel/linear and rotational type flow chambers are developed. We have compared the initial phase of platelet thrombus formation in a parallel plate flow chamber (PPC) and a cone-and-plate chamber (CPC) under von Willebrand dependent shear conditions. Blood was allowed to flow through human collagen type III surfaces at a shear rate of 1000 s(-1) for 150 s. Thrombus deposition was characterized by surface coverage, average area and height of thrombi. VWF distribution within thrombi was analyzed with confocal laser scanning microscopy. Reduced surface-specific platelet adhesion and aggregation (surface coverage and average thrombus size) were observed in CPC along with a significant increase in single platelet disappearance from the circulating blood. Our data suggest that the higher rate of platelet consumption in this device, as opposed to PPC, is limiting the adhesion to the surface. Consequently, surface-specific processes and aggregation in the flowing blood are both assessed using CPC, while comprehensive evaluation of surface-specific processes is best achieved with PPC. Therefore, the choice of chamber type as a diagnostic tool is purpose-dependent.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban blood chemistry Collagen Type III Computer-Assisted Confocal Hemorheology Human Humans Hungary Image Processing instrumentation Linear Models methods Microscopy Non-U.S.Gov't physiology Platelet Adhesiveness Platelet Aggregation Research Research Support Thrombosis von Willebrand Factor
Megjelenés:	Platelets. - 17 : 3 (2006), p. 185-190. -
További szerzők:	Oparaugo, Peter Udvardy Miklós László (1977-) (orvos, tudományos segédmunkatárs) Tóth Judit (1978-) (laboratóriumi szakorvos) Szántó Tímea Daróczi Lajos (1965-) (fizikus) Vereb György (1965-) (biofizikus, orvos) Hársfalvi Jolán (1949-) (klinikai biokémikus)
Internet cím:	elektronikus változat DOI elektronikus változat
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