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001-es BibID:	BIBFORM005964
Első szerző:	Aszalos Adorján
Cím:	Depolymerization of microtubules alters membrane potential and affects the motional freedom of membrane proteins / Adorjan Aszalos, Sandor Damjanovich, Michael M. Gottesman
Dátum:	1986
Megjegyzések:	Two independent lines of evidence were obtained indicating that microtubule depolymerization affects the functions and the physical state of membranes in intact Chinese hamster ovary cells. The first type of evidence was obtained by using the dye dihexyloxacarbocyanine iodide to measure membrane potential before and after treatment with several microtubule active agents. Microtubule depolymerization resulted in a decrease in cell fluorescence, whereas stabilization of microtubules with taxol resulted in an increase in cell fluorescence. These effects of the drugs were due to their interactions with microtubules and not to direct effects of the drugs on the plasma membranes for the following reasons: effects were time dependent and required entry into the cells as indicated by the lack of fluorescence change in a multi-drug-resistant mutant that does not accumulate antimicrotubule drugs and a colcemid-resistant tubulin mutant did not show these effects on cell fluorescence. Evidence for altered motional freedom of membrane proteins in the plasma membrane was obtained by using electron spin resonance analysis of maleimide spin probe labeled cells. This study showed that depolymerization of microtubules results in increased motional freedom of maleimide-labeled sulfhydryl group containing proteins. Taken together, these data argue that microtubules function in mammalian cells to regulate the physical state of membranes and modulate membrane potential generated across cell membranes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban analysis Animal Cell Line Cell Membrane Cricetulus Electron Spin Resonance Spectroscopy Female Fluorescence Hamsters Kinetics Membrane Potentials Membrane Proteins metabolism methods Microtubules Ovary physiology ultrastructure
Megjelenés:	Biochemistry. - 25 : 19 (1986), p. 5804-5809. -
További szerzők:	Damjanovich Sándor (1936-2017) (biofizikus) Gottesman, Michael M.
Internet cím:	elektronikus változat
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