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001-es BibID:	BIBFORM006002
035-os BibID:	(scopus)0023112835 (wos)A1987G382700017
Első szerző:	Szabó Gábor (biofizikus)
Cím:	Overall changes in chromatin sensitivity to DNase I during differentiation / Gábor Szabó, Sandor Damjanovich, János Sümegi, George Klein
Dátum:	1987
Megjegyzések:	The DNase I sensitivity of total chromatin was studied in fixed cells and nuclei isolated from proliferating and terminally differentiated cells, by measuring the incorporation of labelled nucleotides into DNase-sensitive sites, and electrophoresis of DNA isolated from DNase-treated nuclei. The unfixed nuclei were sensitive to digestion at around 10 micrograms/ml, the fixed cells at 30 ng/ml DNase I concentration. Proliferating Rauscher leukemia cells were more digestible than normal spleen cells. The DNase I sensitivity of the human HL60 leukemia line decreased upon DMSO-induced differentiation but still exceeded the digestibility of nuclei from normal human peripheral blood. A novel flow-cytometric technique was developed to study DNase sensitivity at the cell level. It confirmed the relative resistance of differentiated cells to DNase I and ruled out the possibility that this could be due to an altered distribution of cell cycle phases. The overall DNase I sensitivity of chromatin was compared with the sensitivity of the c-myc gene and the myc-associated hypersensitive sites. The latter sites were detected at 1 microgram/ml DNase I in HL60 nuclei. They disappeared partially upon DMSO-induced differentiation. At 10 micrograms/ml, myc was degraded in both growing and differentiating HL60, but not in HPB cells. These data suggest that a progressive condensation of the chromatin occurs during terminal differentiation which gradually involves specific genes that need to be inactivated.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Animal blood Cell Cycle Cell Differentiation Cell Division Cell Line Chromatin cytology Deoxyribonuclease I Dna Flow Cytometry Human Leukemia,Erythroblastic,Acute Leukemia,Experimental metabolism Mice Mice,Inbred BALB C pathology Spleen Support,Non-U.S.Gov't Support,U.S.Gov't,P.H.S. Translation,Genetic ultrastructure
Megjelenés:	Experimental Cell Research. - 169 : 1 (1987), p. 158-168. -
További szerzők:	Damjanovich Sándor (1936-2017) (biofizikus) Sümegi János Klein, George
Internet cím:	elektronikus változat DOI
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