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001-es BibID:	BIBFORM006032
Első szerző:	Delcros, J. G.
Cím:	Differential effects of spermine and its analogues on the structures of polynucleotides complexed with ethidium bromide / Delcros J. G., Sturkenboom M. C., Basu, H. S., Shafer R. H., Szöllösi, J., Feuerstein, B. G., Marton, L. J.
Dátum:	1993
Megjegyzések:	The interactions of spermine and polyamine analogues with synthetic polynucleotides of various base sequences complexed with ethidium bromide (EB) were investigated using measurements of fluorescence intensity and steady-state fluorescence polarization. Spermine and polyamine analogues displaced some but not all of the EB bound to poly(dA-dT).poly(dA-dT) or poly(dG-dC).poly(dG-dC), suggesting that polyamines may stabilize these polynucleotides in a conformation with reduced affinity for EB. Modifications of the aliphatic backbone of spermine have pronounced effects on its ability to displace EB from poly(dA-dT).poly(dA-dT) but not from poly-(dG-dC).poly(dG-dC). Spermine and some but not all of the polyamine analogues caused fluorescence depolarization when they interacted with the complex of EB and poly(dA-dT).poly-(dA-dT). Neither spermine nor any of the analogues, however, induced fluorescence depolarization in the complex of EB with poly(dG-dC).poly(dG-dC) or poly(dA).poly(dT). This suggests that spermine and some spermine analogues induce structural changes specific to alternating A-T sequences.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk chemistry Ethidium Fluorescence Fluorescence Polarization Nucleic Acid Conformation pharmacology Poly dA-dT Polyamines Polydeoxyribonucleotides Polynucleotides Spectrometry,Fluorescence Spermine Support,Non-U.S.Gov't Support,U.S.Gov't,P.H.S.
Megjelenés:	The Biochemical Journal. - 291 : Pt 1 (1993), p. 269-274. -
További szerzők:	Sturkenboom, M. C. Basu, Hirak S. Shafer, R. H. Szöllősi János (1953-) (biofizikus) Feuerstein, Burt G. Marton, Laurence J.
Internet cím:	elektronikus változat
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