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001-es BibID:	BIBFORM006188
Első szerző:	Damante, Chiara A.
Cím:	The metal loading ability of beta-Amyloid N-Terminus : a combined potentiometric and spectroscopic study of Copper(II) complexes with beta-Amyloid(1-16), its short or mutated peptide fragments, and its polyethylene glycol (PEG)-ylated analogue / Damante, C. A., Osz, K., Nagy, Z., Pappalardo, G., Grasso, G., Impellizzeri, G., Rizzarelli, E., Sovago, I.
Dátum:	2008
ISSN:	0020-1669
Megjegyzések:	Alzheimer's disease (AD) is becoming a rapidly growing health problem, as it is one of the main causes of dementia in the elderly. Interestingly, copper(II) (together with zinc and iron) ions are accumulated in amyloid deposits, suggesting that metal binding to A beta could be involved in AD pathogenesis. In A beta, the metal binding is believed to occur within the N-terminal region encompassing the amino acid residues 1-16. In this work, potentiometric, spectroscopic (UV-vis, circular dichroism, and electron paramagnetic resonance), and electrospray ionization mass spectrometry (ESI-MS) approaches were used to investigate the copper(II) coordination features of a new polyethylene glycol (PEG)-conjugated A beta peptide fragment encompassing the 1-16 amino acid residues of the N-terminal region (A beta(1 - 16)PEG). The high water solubility of the resulting metal complexes allowed us to obtain a complete complex speciation at different metal-to-ligand ratios ranging from 1:1 to 4:1. Potentiometric and ESI-MS data indicate that A beta(1-16)PEG is able to bind up to four copper(II) ions. Furthermore, in order to establish the coordination environment at each metal binding site, a series of shorter peptide fragments of A beta, namely, A beta(1-4), A beta(1-6), AcA beta(1-6), and AcA beta(8-16)Y10A, were synthesized, each encompassing a potential copper(II) binding site. The complexation properties of these shorter peptides were also comparatively investigated by using the same experimental approach.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Inorganic Chemistry. - 47 : 20 (2008), p. 9669-9683. -
További szerzők:	Ősz Katalin (1975-) (vegyész) Nagy Zoltán (1974-) (vegyész) Pappalardo, Giuseppe Grasso, Giulia Impellizzeri, Giuseppe Rizzarelli, Enrico Sóvágó Imre (1946-) (vegyész)
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