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001-es BibID:	BIBFORM006790
Első szerző:	Sárváry Attila (népegészségtan szakorvos)
Cím:	Possible role of factor XIII subunit A in Fcgamma and complement receptor-mediated phagocytosis / Sarvary, A., Szucs, S., Balogh, I., Becsky, A., Bardos, H., Kavai, M., Seligsohn, U., Egbring, R., Lopaciuk, S., Muszbek, L., Adany, R.
Dátum:	2004
ISSN:	0008-8749
Megjegyzések:	Besides its traditional role in hemostasis, factor XIII subunit A (FXIII-A) is supposed to function as a cellular transglutaminase and to be involved in certain intracellular processes, including cytoskeletal remodeling. To investigate its intracellular role, the aim of the present study was to follow changes in FXIII-A production in combination with the receptor-mediated phagocytic activities of monocytes/macrophages and to examine the phagocytic functions of monocytes in patients with FXIII-A deficiency. Human blood monocytes were isolated from the buffy coats of healthy volunteers and cultured for 4 days. The FcgammaR-mediated phagocytosis of sensitized erythrocytes (EA) and the complement receptor (CR)-mediated phagocytosis of complement-coated yeast particles were studied during monocyte/macrophage differentiation. Changes in the gene expression of FXIII-A were detected by real-time quantitative RT-PCR. FXIII-A protein production was investigated with fluorescent image analysis at single cell level and Western immunoblot analysis. Both the FcgammaR and CR-mediated phagocytosis increased during culturing, which peaked on day 3. The phagocytic activity of the cells could be markedly inhibited with monodansylcadaverine, an inhibitor of the transglutaminase-induced crosslinking of proteins. The phagocytosis of EA, complement-coated and uncoated yeast particles was found to be strongly diminished in monocytes of FXIII-A deficient patients. The phagocytic functions of cultured cells showed a change in parallel with the alterations in FXIII-A mRNA expression, as well as with that in FXIII-A in protein synthesis detected by image and Western immunoblot analyses in concert. Our results suggest that FXIII-A plays a role in the Fcgamma and complement receptor-mediated phagocytic activities of monocytes/macrophages.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Blotting, Western Cadaverine Enzyme Inhibitors Erythrocytes Factor XIII Female Humans Macrophages Male Microscopy, Fluorescence Phagocytosis RNA Receptors, Complement 3b Receptors, IgG Reverse Transcriptase Polymerase Chain Reaction Transglutaminases
Megjelenés:	Cellular Immunology. - 228 : 2 (2004), p. 81-90. -
További szerzők:	Szűcs Sándor (1958-) (biokémikus, vegyész) Balogh Imre Becsky Áron Bárdos Helga (1969-) (megelőző orvostan és népegészségtan szakorvos) Kávai Mária (1930-) (vegyész) Seligsohn, Uri Egbring, Rudolf Lopaciuk, Stanislaw Muszbek László (1942-) (haematológus, kutató orvos) Ádány Róza (1952-) (megelőző orvostan és népegészségtan szakorvos)
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