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001-es BibID:	BIBFORM008346
Első szerző:	Binod, Parameswaran
Cím:	Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation / Parameswaran Binod, Tünde Pusztahelyi, Viviana Nagy, Chandran Sandhya, George Szakács, István Pócsi, Ashok Pandey
Dátum:	2005
Megjegyzések:	Fourteen Penicillium strains have been screened on wheat bran-crude chitin mixture medium for extracellular chitinase production in solid-state fermentation. Under the experimental conditions tested, Penicillium aculeatum NRRL 2129 (=ATCC 10409) was selected as the best enzyme producer. The optimum incubation period for chitinase production by the potent organism was found to be 72 h. Chromatofocusing was performed as the first step in the purification scheme, but high amount of contaminating proteins interfered with the method. Hence, ion-exchange chromatography experiments were carried out followed by gel filtration to separate and isolate chitinase isoenzymes. Four major chitinase peaks of molecular weight 82.7, 44.6, 28.2 and 26.9 kDa were observed after gel filtration chromatography while, on SDS-PAGE,three protein bands of molecular weights 82.6, 33.9 and 29.1 kDa were identified. The purified enzyme showed optimal temperature and pH at 50 and 5.5 °C, respectively.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Enzyme and Microbal Technology. - 36 : 7 (2005), p. 880-887. -
További szerzők:	Pusztahelyi Tünde (1969-) (biológus, angol-magyar szakfordító) Nagy Viviana Sandhya, Chandran Szakács György Pócsi István (1961-) (vegyész) Pandey, Ashok
Internet cím:	DOI elektronikus változat
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