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001-es BibID:	BIBFORM009182
Első szerző:	Oddoux, Sarah
Cím:	Triadin deletion induces impaired skeletal muscle function / Oddoux, S., Brocard, J., Schweitzer, A., Szentesi, P., Giannesini, B., Brocard, J., Faure, J., Pernet-Gallay, K., Bendahan, D., Lunardi, J., Csernoch, L., Marty, I.
Dátum:	2009
ISSN:	1083-351X
Megjegyzések:	Triadin is a multiple proteins family, some isoforms being involved in muscle excitation-contraction coupling, and some having still unknown functions. In order to obtain clues on triadin functions, we engineered a triadin knock out mouse line, and characterized the physiological effect of triadin ablation on skeletal muscle function. These mice presented a reduced muscle strength, which seemed not to alter their survival, and which has been characterized in the present work. We first checked in these mice the expression level of the different proteins involved in calcium homeostasis, and observed in fast muscles an increase in expression of dihydropyridine receptor, with a large reduction in calsequestrin expression. Electron microscopy analysis of KO muscles morphology demonstrated the presence of triads in abnormal orientation, and a reduction in the sarcoplasmic reticulum terminal cisternae volume. Using calcium imaging on cultured myotubes, we observed a reduction in the total amount of calcium stored in the sarcoplasmic reticulum. Physiological studies have been performed to evaluate the influence of triadin deletion on skeletal muscle function. Muscle strength has been measured both on whole animal, using hang test or electrical stimulation combined with NMR analysis and strength measurement, or on isolated muscle using electrical stimulation. All the results obtained demonstrate an important reduction in muscle strength, indicating that triadin plays an essential role in skeletal muscle function and in skeletal muscle structure. These results indicate that triadin alteration lead to the development of a myopathy, which could be studied using this new animal model.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal of Biological Chemistry. - 284 : 50 (2009), p. 34918-34929. -
További szerzők:	Brocard, Julie Schweitzer, Annie Szentesi Péter (1967-) (élettanász) Giannesini, Benoit Brocard, Jacques Faure, Julien Pernet-Gallay, Karine Bendahan, David Lunardi, Joel Csernoch László (1961-) (élettanász) Marty, Isabelle
Internet cím:	DOI elektronikus változat
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