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001-es BibID:	BIBFORM010362
Első szerző:	Székvölgyi Lóránt (biofizikus, biokémikus, sejtbiológus)
Cím:	Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research / Lorant Szekvolgyi, Laszlo Imre, Doan Xuan Quang Minh, Eva Hegedus, Zsolt Bacso, Gabor Szabo
Dátum:	2009
ISSN:	9781603274135
Megjegyzések:	Our understanding of epigenetics has been transformed in recent years by the advance of technological possibilities based primarily on a powerful tool, chromatin immunoprecipitation (ChIP). However, in many cases, the detection of epigenetic changes requires methods providing a high-throughput (HTP) platform. Cytometry has opened a novel approach for the quantitative measurement of molecules, including PCR products, anchored to appropriately addressed microbeads (Pataki et al. 2005. Cytometry 68, 45-52). Here we show selected examples for the utility of two different cytometry-based platforms of epigenetic analysis: ChIP-on-beads, a flow-cytometric test of local histone modifications (Balint et al. 2005. Mol. Cell Biol. 25, 5648-5663), and the laser scanning cytometry-based measurement of global epigenetic modifications that might help predict clinical behavior in different pathological conditions. We anticipate that such alternative tools may shortly become indispensable in clinical practice, translating the systematic screening of epigenetic tags from basic research into routine diagnostics of HTP demand.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Chromatin Chromatin Immunoprecipitation Immunoprecipitation methods
Megjelenés:	Methods in Molecular Biology. - 567 (2009), p. 99-111. -
További szerzők:	Imre László (1979-) (biológus) Doan-Xuan, Quang-Minh (1986-) (biofizikus) Hegedűs Éva (1978-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Szabó Gábor (1953-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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