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001-es BibID:	BIBFORM015619
Első szerző:	Fábián Ákos István (aneszteziológus)
Cím:	Strength in numbers : effects of Acceptor Abundance on FRET Efficiency / Ákos I. Fábián, Tünde Rente, János Szöllősi, László Mátyus, Attila Jenei
Dátum:	2010
Megjegyzések:	Fluorescence resonance energy transfer (FRET) is a strongly distance-dependent process between a donor and an acceptor molecule, which can be used for sensitive distance measurements and characterization of molecular interactions at the nanometer level. The original mathematical description of this process, however, is only valid for the interaction of one donor with one acceptor. This criterion is not always met, especially in biological systems, where multiple structures can interact simultaneously, often making distance estimations based on transfer efficiency values error-prone. Herein we investigate how the interaction of multiple acceptors and donors influences the transfer efficiency value in an intramolecular cellular FRET system by manipulating the fluorophore/protein ratio of the fluorophore-conjugated antibodies. We show that the labeling ratio of the acceptor has the largest influence on measured transfer efficiency and decreasing or increasing the acceptor labeling ratio can be utilized to manipulate the FRET response of the acceptor-donor pair and therefore is a tool for optimizing sensitivity of FRET measurements
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Antibodies article BINDING-SITE BY-CELL BASIS cell surface receptors chemistry Energy Transfer Molekuláris Medicina FLOW Flow Cytometry Fluorescence FLUORESCENCE RESONANCE Fluorescence Resonance Energy Transfer FRET HIGH-THROUGHPUT FRET Hungary IMAGING MICROSCOPY LIVING CELLS molecular interaction PROTEIN INTERACTIONS resonance energy transfer RESONANCE ENERGY-TRANSFER SPECTROSCOPIC RULER OTKA::1 MAB::3.1 egyetemen (Magyarországon) készült közlemény
Megjelenés:	Chemphyschem. - 11 : 17 (2010), p. 3713-3721. -
További szerzők:	Rente Tünde Szöllősi János (1953-) (biofizikus) Mátyus László (1956-) (biofizikus) Jenei Attila (1966-) (biofizikus)
Pályázati támogatás:	TÁMOP-4.2.1/B-09/1/KONV-2010-0007 TÁMOP Membrán dinamika K 77600 OTKA K 68763 OTKA TÁMOP-4.2.2-08/1-2008-0019 TÁMOP
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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