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001-es BibID:	BIBFORM015664
Első szerző:	Barok Márk (biofizikus)
Cím:	Characterization of a novel, trastuzumab resistant human breast cancer cell line / Barok Mark, Balazs Margit, Lazar Viktoria, Rakosy Zsuzsa, Toth Eniko, Treszl Andrea, Vereb Gyorgy, Colbern G. T., Park J. W., Szollosi Janos
Dátum:	2010
ISSN:	1945-0508
Megjegyzések:	HER2-positive breast cancers represent a distinct phenotype and are intrinsically more aggressive than HER2-negative tumors. Although HER2-targeted therapies have been rationally developed, resistance to these treatments represents a process understood poorly. There are few experimental models that allow studying the molecular mechanism of resistance. Our aim was to characterize a trastuzumab resistant breast cancer cell line (B585) that was established from an invasive ductal carcinoma. B585 grows only in immunodeficient mice as a xenograft. CGH and FISH were used to define cytogenetic alterations, gene-expression analysis and immunohistochemistry were applied to detect RNA and protein expression. By array-CGH focused amplifications were identified for C-MYC, EGFR, ErbB2, CCND1 and TOP2-A oncogenes. ErbB2 was co-amplified with TOP2-A. mRNA overexpression was detected for the amplified genes. ErbB2 protein was overexpressed and showed heterogeneous distribution. In summary, molecular cytogenetic analysis and expression profiling of B585 revealed several new alterations. Based on the experiments performed in SCID mice and the genotypic/phenotypic characteristics, this new in vivo breast cancer xenograft is a valuable model to investigate molecular mechanism of trastuzumab resistance
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk analysis Animals Antibodies Antibodies,Monoclonal Antineoplastic Agents article Biophysics Breast Neoplasms Carcinoma Carcinoma,Ductal,Breast Cell Line Cell Line,Tumor Comparative Genomic Hybridization Disease Models,Animal Drug Resistance,Neoplasm drug therapy EGFR ErbB2 Female Gene Expression Gene Expression Profiling genetics Human Humans Hungary Immunohistochemistry In Situ Hybridization,Fluorescence metabolism Mice Mice,Scid Oncogenes Phenotype Receptor,erbB-2 Research Research Support rna Support therapeutic use therapy Trastuzumab resistance OTKA::1 MAB::3.1
Megjelenés:	Frontiers In Bioscience (elite edition). - 1 : 2 (2010), p. 627-640. -
További szerzők:	Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Lázár Viktória (1981-) (molekuláris biológus) Rákosy Zsuzsa (1978-) (sejtbiológus, molekuláris biológus, genetikus) Tóth Enikő Treszl Andrea (1974-) (molekuláris biológus) Vereb György (1965-) (biofizikus, orvos) Colbern, Gail T. Park, John W. Szöllősi János (1953-) (biofizikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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