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001-es BibID:	BIBFORM015936
Első szerző:	Fülöp Csaba
Cím:	Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice / Csaba Fülöp, Sándor Szántó, Durba Mukhopadhyay, Tamás Bárdos, Rajesh V. Kamath, Marylin S. Rugg, Anthony J. Day, Antonietta Salustri, Vincent C. Hascall, Tibor T. Glant, Katalin Mikecz
Dátum:	2003
ISSN:	0950-1991 (Print)
Megjegyzések:	Mucification of the cumulus layer around the oocyte is an obligatory process for female fertility. Tumor necrosis factor-induced protein-6 (TNFIP6 or TSG6) has been shown to be specifically expressed during this process. We have generated TNFIP6-deficient mice and tested the ability of their cumulus cells to undergo mucification. Cumulus cell-oocyte complexes fail to expand in TNFIP6-deficient female mice because of the inability of the cumulus cells to assemble their hyaluronan-rich extracellular matrix. The impaired cumulus matrix formation is due to the lack of covalent complexes between hyaluronan and the heavy chains of the inter-alpha-trypsin inhibitor family. As a consequence, TNFIP6-deficient females are sterile. Cultured TNFIP6-deficient cumulus cell-oocyte complexes also fail to expand when stimulated with dibutyryl cyclic AMP or epidermal growth factor. Recombinant TNFIP6 is able to catalyze the covalent transfer of heavy chains to hyaluronan in a cell-free system, restore the expansion of Tnfip6-null cumulus cell-oocyte complexes in vitro, and rescue the fertility in Tnfip6-null females. These results provide clear evidence that TNFIP6 is a key catalyst in the formation of the cumulus extracellular matrix and indispensable for female fertility.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban Animals Cell Adhesion Molecules/genetics/metabolism Extracellular Matrix/metabolism Female Genotype Humans Hyaluronic Acid/chemistry/metabolism Infertility, Female Male Mice Mice, Knockout Oocytes/cytology/*physiology Ovary/anatomy & histology/pathology Recombinant Proteins/genetics/metabolism
Megjelenés:	Development. - 130 : 10 (2003), p. 2253-2261. -
További szerzők:	Szántó Sándor (1968-) (belgyógyász, reumatológus) Mukhopadhyay, Durba Bárdos Tamás Kamath, Rajesh V. Rugg, Marylin S. Day, Anthony J. Salustri, Antonietta Hascall, Vincent C. Glant Tibor T. Mikecz Katalin
Internet cím:	elektronikus változat DOI Intézményi repozitóriumban (DEA) tárolt változat
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