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001-es BibID:	BIBFORM018107
Első szerző:	Portnoy, Thomas
Cím:	The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilation / Thomas Portnoy, Antoine Margeot, Rita Linke, Lea Atanasova, Erzsébet Fekete, Erzsébet Sándor, Lukas Hartl, Levente Karaffa, Irina S Druzhinina, Bernhard Seiboth, Stéphane Le Crom, Christian P Kubicek
Dátum:	2011
ISSN:	1471-2164
Megjegyzések:	Background The identification and characterization of the transcriptional regulatory networks governing the physiology and adaptation of microbial cells is a key step in understanding their behaviour. One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. However, the complete list of its gene targets is not known. Results Here, we deciphered the CRE1 regulatory range in the model cellulose and hemicellulose-degrading fungus Trichoderma reesei (anamorph of Hypocrea jecorina) by profiling transcription in a wild-type and a delta-cre1 mutant strain on glucose at constant growth rates known to repress and de-repress CCR-affected genes. Analysis of genome-wide microarrays reveals 2.8% of transcripts whose expression was regulated in at least one of the four experimental conditions: 47.3% of which were repressed by CRE1, whereas 29.0% were actually induced by CRE1, and 17.2% only affected by the growth rate but CRE1 independent. Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. In addition, we found CRE1-repression of nitrogenous substances uptake, components of chromatin remodeling and the transcriptional mediator complex, as well as developmental processes. Conclusions Our study provides the first global insight into the molecular physiological response of a multicellular fungus to carbon catabolite regulation and identifies several not yet known targets in a growth-controlled environment.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	BMC Genomics. - 12 : 269 (2011), p. 1-12. -
További szerzők:	Margeot, Antoine Linke, Rita Atanasova, Lea Fekete Erzsébet (1975-) (biotechnológus, egyetemi tanár) Karaffa Erzsébet Mónika (1972-) (mikrobiológus, egyetemi tanár) Hartl, Lukas Karaffa Levente (1971-) (biotechnológus, egyetemi tanár) Druzhinina, Irina S. Seiboth, Bernhard Le Crom, Stéphane Kubicek, Christian P.
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