Találati lista | Tudóstér

001-es BibID:	BIBFORM020318
Első szerző:	Jakab Balázs (Pécs)
Cím:	Distribution of PACAP-38 in the central nervous system of various species determined by a novel radioimmunoassay / Balázs Jakab, Dóra Reglődi, Rita Józsa, Tibor Hollósy, Andrea Tamás, Andrea Lubics, István Lengvári, Gábor Oroszi, Zoltán Szilvássy, János Szolcsányi, József Németh
Dátum:	2004
Megjegyzések:	AbstractPituitary adenylate cyclase activating polypeptide (PACAP) occurs in two molecular forms: PACAP-38 and PACAP-27. Soon after the isolation and chemical characterization of PACAP, the first radioimmunoassay (RIA) methods have been developed, but it is a still rarely used laboratory technique in the field of PACAP research. The aim of the present study was to develop a novel, highly specific PACAP-38 assay to investigate the quantitative distribution of PACAP-38 in the central nervous system of various vertebrate species under the same technical and experimental conditions. Different areas of the brain and the spinal cord were removed from rats, chickens and fishes and the tissue samples were processed for PACAP-38 RIA. Our results indicate that the antiserum used in the RIA is C-terminal specific, without affinity for other members of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon peptide family. The average ID50 value was 48.6+/-3.4 fmol/ml determined in 10 consecutive assays. Detection limit for PACAP-38 proved to be 2 fmol/ml. PACAP-38 immunoreactivity was present in the examined brain areas of each species studied, with highest concentration in the rat diencephalons. High levels of PACAP-38 were also detected in the rat telencephalon, followed by spinal cord and brainstem. The central nervous system of the fish also contained considerable concentrations of PACAP-38, whereas lowest concentrations were measured in the central nervous system of the chicken.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban PACAP-38 central nervous system radioimmunoassay
Megjelenés:	Journal of Biochemical and Biophysical Methods. - 61 : 1-2 (2004), p. 189-198. -
További szerzők:	Reglődi Dóra (Idegtudományok) Józsa Rita (Pécs) Hollósy Tibor (Pécs) Tamás Andrea (Idegtudomány) (Pécs) Lubics Andrea (Pécs) Lengvári István Oroszi Gábor (Pécs) Szolcsányi János (Pécs) Németh József (Pécs) Szilvássy Zoltán (1957-) (belgyógyász, farmakológus, klinikai farmakológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon: