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001-es BibID:	BIBFORM027871
Első szerző:	Csordás Tóth Éva
Cím:	Protein phosphatase 2A holoenzyme and its subunits from Medicago sativa / Éva Csordás Tóth, Emese Vissi, Izabella Kovács, Attila Szőke, Joaquín Ariño, Pál Gergely, Dénes Dudits, Viktor Dombrádi
Dátum:	2000
ISSN:	0167-4412
Megjegyzések:	We detected an about 200 kDa holoenzyme of protein phosphatase 2A (PP2A) in the crude extract of Medicago sativa microcallus cells by gel permeation chromatography. By polymerase chain reaction (PCR) we isolated two M. sativa cDNA fragments corresponding to the catalytic (C) subunit, and one each coding for the A and the B regulatory subunits of PP2A. The C subunit sequences were different from that published previously, indicating the existence of at least three different isoforms in M. sativa. Using the PCR fragments as probes, we obtained two distinct full-length clones for both the A and B subunits from an alfalfa cDNA library. Our results demonstrate that the components of the PP2A holoenzyme, namely the catalytic and regulatory subunits, are present in alfalfa in several isoforms and that their sequences are highly similar to their plant, yeast and animal counterparts. The distinct regulatory subunit genes are constitutively expressed during the cell cycle. Interestingly, two A-B subunit pairs had parallel mRNA steady-state levels in different plant tissues suggesting that not all of the possible isoform combinations are present in all tissues. The expression of the MsPP2A Bbeta subunit form was induced by abscisic acid indicating a specific function for this protein in the stress response.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban cDNA cloning quaternary structure Ser/Thr protein phosphatase stress response tissue-specific expression
Megjelenés:	Plant Molecular Biology. - 43 : 4 (2000), p. 527-536. -
További szerzők:	Kovács Izabella (Szeged) Szőke Attila Ariño, Joaquín Dudits Dénes Vissi Emese (1968-) (biokémikus, biológus) Gergely Pál (1947-) (biokémikus) Dombrádi Viktor (1953-) (biokémikus)
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat DOI
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